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Expression

Animal Genetics   Research Article

Differential expression of the lethal gene Luteus-Pa in cacao of the Parinari series

Authors: B.C. Rehem1, A.-A.F. Almeida1, G.S.F. Figueiredo1, A.S. Gesteira2, S.C. Santos1, R.X. Corr?ªa1, M.M. Yamada3 and R.R. Valle3

The recessive lethal character Luteus-Pa is found in cacao (Theobroma cacao) genotypes of the Parinari series (Pa) and is characterized by expression of leaf chlorosis and seedling death. Several genotypes of the Pa series are bearers of the gene responsible for the expression of the Luteus-Pa character, which can be used as a tool for determining relatio.. Read More»

Genet. Mol. Res. 15(1):
gmr.15014925
DOI:
10.4238/gmr.15014925
Plant Genetics   Research Article

Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR

Authors: H.-J. Xiao, J.-H. Jin, W.-G. Chai and Z.-H. Gong

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L... Read More»

Genet. Mol. Res. 14(1):
2015.January.23.10
DOI:
10.4238/2015.January.23.10
Bioinformatics   Research Article

Optimal clone identifier for genomic shotgun libraries: â??OC Identifier toolâ?

Authors: Cantao ME, Ferreira JE and Lemos EGM

In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of puttin.. Read More»

Genet. Mol. Res. 6(4):
Microbial Genetics   Research Article

Genome-wide partial correlation analysis of Escherichia coli microarray data

Authors: Veiga DFT, Vicente FFR, Grivet M, de la Fuente A and Vasconcelos ATR

Transcriptional control is an essential regulatory mechanism employed by bacteria. Much about transcriptional regulation remains to be discovered, even for the most widely studied bacterium, Escherichia coli. In the present study, we made a genome-wide loworder partial correlation analysis of E. coli microarray data with the p.. Read More»

Genet. Mol. Res. 6(4):
Animal Genetics   Research Article

Expression of the ROR?± gene in Inner Mongolian cashmere goat hair follicles

Authors: Y.H. Zhao, Z.H. Liu, L. Wang, H.M. Xiao, C.G. Du, Y.J. Zhang,R. Su and J.Q. Li

The expression of retinoid-acid-related orphan receptor ?± (ROR?±) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that ROR?±was expressed.. Read More»

Genet. Mol. Res. 14(1):
2015.January.23.11
DOI:
10.4238/2015.January.23.11
Human Genetics   Research Article

Molecular characterization, tissue expression, and polymorphism analysis of liver-type fatty acid binding protein in Landes geese

Authors: Z. Song, D. Shao, X.X. Sun, J.W. Niu and D.Q. Gong

Liver weight is an important economic trait in the fatty goose liver industry. Liver-type fatty acid binding protein (L-FABP) is involved in the formation and metabolism of fatty acids. Thus, we hypothesized that sequence polymorphisms in L-FABP were associated with fatty liver weight in goose. We first isolated, sequenced, and characterized the goose L-F.. Read More»

Genet. Mol. Res. 14(1):
2015.January.23.12
DOI:
10.4238/2015.January.23.12
Animal Genetics   Research Article

Differential expression of peroxisome proliferator-activated receptor ?³, fatty acid synthase, and hormone-sensitive lipase in fat-tailed and thin-tailed sheep breeds

Authors: X.C. Xu, B.B. Li, X. Wei, Y.X. Yang, X.L. Wang, Y.L. Chen

Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan shee.. Read More»

Genet. Mol. Res. 14(4):
Human Genetics   Research Article

DAP1 high expression increases risk of lymph node metastases in squamous cell carcinoma of the oral cavity

Authors: M. Santos, L.L. Maia, C.V.M. Silva, G.T. Peterle, A.M.C. Mercante, F.D. Nunes, M.B. Carvalho, E.H. Tajara, I.D. Louro and A.M.A. Silva-Conforti

Death-associated protein 1 (DAP1) is a member of the DAP family. Its expression is associated with cell growth and normal death of the neoplastic cells, regulated by the mammalian target of the rapamycin protein. Activated DAP1 negatively regulates autophagy, which has been associated with the development and progression of several diseases, such as cance.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.8.13
DOI:
http://dx.doi.org/10.4238/2015.September.8.13
Animal Genetics   Research Article

mRNA and protein expression levels of four candidate genes for ear size in Erhualian and Large White pigs

Authors: L.C. Zhang, J. Liang, L. Pu, Y.B. Zhang, L.G. Wang, X. Liu, H. Yan, L.X. Wang

Porcine ear size is an important characteristic for distinguishing among pig breeds. In a previous genome-wide association study of porcine ear size, LEM domain-containing 3 (LEMD3), methionine sulfoxide reductase B3 (MSRB3), high mobility group AT-hook 2 (HMGA2), and Wnt inhibitory factor 1 (WIF1) were implicated as important.. Read More»

Genet. Mol. Res. 16(2):
gmr16029252
DOI:
10.4238/gmr16029252
Animal Genetics   Research Article

Molecular cloning, expression, and regulation of the ovalbumin gene in pigeon oviduct epithelial cells

Authors: H. Zhang, L.Z. Lu, L. Chen, Z.R. Tao, F. Chen, S.L. Zhong,Y.L. Liu, Y. Tian and P.S. Yan

The full-length pigeon ovalbumin (OVA) gene cDNA was cloned and sequenced by reverse transcription-polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends. A 386-amino acid protein was predicted for the obtained sequence, which had 67% identity with the chicken protein. Similar to chicken OVA, the pigeon OVA ge.. Read More»

Genet. Mol. Res. 13(1):
2014.January.10.2
DOI:
10.4238/2014.January.10.2