Expression
Regulation of human alpha-globin gene expression and alpha-thalassemia
Authors: D.M. Ribeiro and M.F. Sonati
Hemoglobin and globin genes are important models for studying protein and gene structure, function and regulation. We reviewed the main aspects of regulation of human α-globin synthesis, encoded by two adjacent genes (α2 and α1) clustered on chromosome 16. Their expression is controlled mainly by a regulatory element located 40 kb upstre.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr472
- DOI:
- 10.4238/vol7-4gmr472
Molecular characterization and tissue expression profile analysis of the porcine JAZF1 gene
Authors: H. Yang, J. He, X.L. Xu, J. Jiang, C.Q. He and H.M. Ma
A recent study indicated that the JAZF1 gene was related to lipid metabolism by regulating the level of gene expression in humans and mice. In order to investigate whether JAZF1 gene expression was associated with fat deposition in pig, we cloned the full-coding region of the JAZF1 gene (GenBank accession No. KF307636) from porcine longissimus dorsi. Resu.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.26.9
- DOI:
- 10.4238/2015.January.26.9
Conjugated linoleic acid-induced milk fat reduction associated with depressed expression of lipogenic genes in lactating Holstein mammary glands
Authors: L.Q. Han K. Pang H.J. Li S.B. Zhu L.F. Wang Y.B. Wang G.Q. Yang G.Y. Yang
The efficacy of conjugated linoleic acid (CLA) in diet supplements for milk fat reduction is well documented in several species. However, the mechanisms by which fatty acids regulate mammary lipogenesis remain largely unknown, especially with regard to gene expression of enzyme and regulators. In this study, 8 Holstein dairy cows in their mid-lactation pe.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.17.2
- DOI:
- 10.4238/2012.September.17.2
Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene
Authors: J.X. Juan, X.H. Yu, X.M. Jiang, Z. Gao, Y. Zhang, W. Li,Y.D. Duan and G. Yang
ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitati.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.1
- DOI:
- 10.4238/2015.January.30.1
Molecular characterization, tissue expression profile, and SNP analysis of porcine SLC13A5
Authors: L.Y. Wang, J. Jiang, H.M. Ma
Solute carrier family 13 (sodium-dependent citrate transporter member 5, SLC13A5) gene has been recently found to play an important role in intramuscular fat content in pigs. In this study, the full-length cDNA of porcine SLC13A5 was obtained from the longissimus dorsi muscle of Shaziling pigs, using the rapid amplification of.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.7.21
- DOI:
- 10.4238/2015.December.7.21
Characterization, molecular cloning, and expression analysis of Ecsit in the spinyhead croaker, Collichthys lucidus
Authors: W. Song, K.J. Jiang, F.Y. Zhang, J. Wang and L.B. Ma
Evolutionarily conserved signaling intermediate in Toll pathways (Ecsit) is reported to play an essential role in innate immunity, embryogenesis, and assembly or stability of the mitochondrial complex I. In this study, the full-length cDNA of Ecsit was cloned from the spinyhead croaker Collichthys lucidus based on the expressed sequence tags from our cDNA.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017193
- DOI:
- 10.4238/gmr.15017193
Identification of hub genes and pathways associated with retinoblastoma based on co-expression network analysis
Authors: Q.L. Wang, X. Chen, M.H. Zhang, Q.H. Shen, Z.M. Qin
The objective of this paper was to identify hub genes and pathways associated with retinoblastoma using centrality analysis of the co-expression network and pathway-enrichment analysis. The co-expression network of retinoblastoma was constructed by weighted gene co-expression network analysis (WGCNA) based on differentially ex.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.8.4
- DOI:
- 10.4238/2015.December.8.4
Genome-wide analysis of immunophilin FKBP genes and expression patterns in Zea mays
Authors: W.-W. Wang, Q. Ma, Y. Xiang, S.-W. Zhu and B.-J. Cheng
The receptors for the immunosuppression drugs FK506 and rapamycin are called FKBPs (FK506-binding proteins). FKBPs comprise a large family; they are found in many species, including bacteria, fungi, animals, and plants. As a class of peptidyl-prolyl cis-trans isomerase enzymes, the FKBP genes have been the focus of recent stud.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.25.2
- DOI:
- 10.4238/2012.June.25.2
Screening and identification of peritoneal metastasis-related genes of gastric adenocarcinoma using a cDNA microarray
Authors: F.H. Bai, N.J. Wang, J. Wang, L. Yang, F.M. Zhang, F. Yin, J. Liang, K.C. Wu and D.M. Fan
With the aim of identifying peritoneal metastasis-related genes in gastric cancer, we performed a broad analysis of differential gene expression between the parental cell line GC9811 and its highly metastatic peritoneal counterpart, cell line GC9811-P. Two fluorescent cDNA probes, labeled with Cy3 and Cy5 dyes, were prepared f.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.25.1
- DOI:
- 10.4238/2012.June.25.1
Molecular cloning and expression analysis of TRAF3 in chicken
Authors: H.L. Yang, Z.Q. Feng, S.Q. Zeng, S.M. Li, Q. Zhu and Y.P. Liu
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.April.30.14
- DOI:
- 10.4238/2015.April.30.14