Expression
Characterization of MUSTN1 gene and its relationship with skeletal muscle development at postnatal stages in Pekin ducks
Authors: T.S. Xu, L.H. Gu, Y. Sun, X.H. Zhang, B.G. Ye, X.L. Liu and S.S. Hou
Musculoskeletal embryonic nuclear protein 1 (MUSTN1) gene is involved in myogenic fusion and differentiation in rats. We previously showed the differential expression of MUSTN1 in week (W) 2 and W6 breast muscles of Pekin ducks. In this study, we further investigated its molecular characteristics and expression profiles in different tissues at W7 and in b.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.2
- DOI:
- 10.4238/2015.May.4.2
Cloning, expression analysis and sequence prediction of the CCAAT/enhancer-binding protein alpha gene of Qinchuan cattle
Authors: H. Wang, L.S. Zan, H.B. Wang, C. Gong and C.Z. Fu
CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor, regulating the differentiation of adipocytes. We cloned the complete open reading frame of C/EBPα gene of Qinchuan cattle and analyzed its protein structures and expression profile in 15 tissues via DNA cloning, sequencing and RT-PC.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.15.14
- DOI:
- 10.4238/2012.June.15.14
Cloning and expression analysis of cysteine protease gene (MwCP) in Agropyron mongolicum Keng
Authors: T.G.B.Y. Ao1,3, M.L. Lang2, Y.Q. Li3, Y. Zhao1, L.C. Wang1 and X.J. Yang1,2,4
In this study, a cysteine protease gene (MwCP) from Agropyron mongolicum Keng was isolated using RACE. Sequence analysis indicated that MwCP was 1473 bp, and it contained a 1134-bp open reading frame, which encoded 377 amino acids with a 24-amino acid N-terminal signal peptide. The results indicated that the MwCP protein was a new member of the papain C1A.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017424
- DOI:
- 10.4238/gmr.15017424
Molecular cloning and expression analysis of GhLOF genes in upland cotton (Gossypium hirsutum L.)
Authors: T.C. Dai and Z.M. Wang
Shoot branching, i.e., the timing and position of shoot growth, determines to a large extend the pattern of plant architecture, and is the result of the integration of a plant’s genetic background and environmental cues. Many genes that are involved in the formation and outgrowth of axillary buds have been cloned, but the exact mechanism is still un.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.16
- DOI:
- 10.4238/2015.May.4.16
Expression divergence of FRUITFULL homeologs enhanced pod shatter resistance in Brassica napus
Authors: P.F. Peng, Y.C. Li, D.S. Mei, J. Colasanti, L. Fu, J. Liu, Y.F. Chen and Q. Hu
To improve pod shatter resistance in the important oilseed crop Brassica napus, the phenotypic diversity of B. napus was tested using 80 B. napus varieties for pod shatter resistance by a random impact test. Among these varieties, R1-1 was identified as resistant, while R2, 8908B was susceptible to shatter. To understand the molecular basis for this pheno.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.2.11
- DOI:
- 10.4238/2015.February.2.11
Construction and expression of prokaryotic expression vectors fused with genes of Magnaporthe oryzae effector proteins and mCherry
Authors: Y.Q. Yang, H. Wang, M.L. Liang, J.L. Yan, L. Liu, C.Y. Li and J. Yang
The aim of the current study was to investigate the prokaryotic expression of the Magnaporthe oryzae effector genes BAS1 and BAS4 fused to the fluorescent protein mCherry. Based on previous polymorphic analysis of BAS1 and BAS4 in rice blast strains using PCR, blast strains containing the PCR products of BAS1 and BAS4 were selected for liquid culture for .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.21
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.21
Application of MUTIC to the exploration of gene expression data in prostate cancer
Authors: Goertzel B, Pennachin C, Coelho LS and Mudado MA
We show here an example of the application of a novel method, MUTIC (model utilization-based clustering), used for identifying complex interactions between genes or gene categories based on gene expression data. The method deals with binary categorical data which consist of a set of gene expression profiles divided into two bi.. Read More»
- Genet. Mol. Res. 6(4):
Molecular characterization, tissue expression profile, and single nucleotide polymorphism analysis of the periostin gene in swine
Authors: D. Xu, H.M. Ma and D.F. Xiao
Periostin, also called osteoblast-specific factor 2, is an important regulator of bone, cardiac development, and wound healing. A recent study revealed that periostin plays an important role in tumor development and is upregulated in a wide variety of cancers. However, little is known about periostin in swine. Therefore, the cDNA sequence of the porcine p.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017187
- DOI:
- 10.4238/gmr.15017187
Decreased MEFV gene expression in rheumatoid arthritis patients
Authors: E.O. Etem, S.S. Koca, D. Erol, S. Yolbas, E. Oz, H. Elyas and A. Is�±k
Rheumatoid arthritis (RA) is a major cause of adult chronic inflammatory arthritis and an autoimmune disease of unknown etiology in which the inflammatory pathology involves T cell activation. Genetic mutations in the Mediterranean fever (MEFV) gene, encoding pyrin, influence the severity of RA, but the underlying mechanisms are not completely understood... Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.3
- DOI:
- 10.4238/2015.February.6.3
Molecular characterization and expression patterns of the big defensin gene in freshwater mussel (Hyriopsis cumingii)
Authors: G.-L. Wang, X.-L. Xia, X.-L. Li, S.-J. Dong and J.-L. Li
Antimicrobial peptides (AMPs), of which big defensins are examples, are an important component of the natural defenses of most living organisms, and possess remarkable microbicidal activities. In the present study, using expressed-sequence tag sequences from a cDNA library and RACE, the full-length cDNA sequence of the big def.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.29.1
- DOI:
- 10.4238/2014.January.29.1