Expression
Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures
Authors: P.A. Auler, L.C. Benitez, M.N. do Amaral, I.L. Vighi, G.S. Rodrigues, L.C. da Maia and E.J.B. Braga
Rice is a cereal that presents a great ability to adapt to different soil and climate conditions. However, as it is a tropical crop with C3 metabolism, it performs better in warm temperatures with high solar radiation. Tolerance to stress caused by low temperatures is a highly complex process that involves various metabolic pa.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029695
- DOI:
- 10.4238/gmr16029695
Differential gene expression in the peripheral blood of Chinese Sanhe cattle exposed to severe cold stress
Authors: Q. Xu, Y.C. Wang, R. Liu, L.F. Brito, L. Kang, Y. Yu, D.S. Wang, H.J. Wu and A. Liu
Livestock is an important food resource for the inhabitants of cold regions, such as northern Asia and alpine regions, where agriculture is limited. In these regions, cold stress largely affects livestock production, thereby reducing the productivity and survival of animals. Despite the importance of breeding cold-tolerant ani.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029593
- DOI:
- 10.4238/gmr16029593
De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress
Authors: A.A. Belesini, F.M.S. Carvalho, B.R. Telles, G.M. de Castro, P.F. Giachetto, J.S. Vantini, S.D. Carlin, J.O. Cazetta, D.G. Pinheiro and M.I.T. Ferro
Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16028845
- DOI:
- 10.4238/gmr16028845
Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements
Authors: N. Marini, C.B. Bevilacqua, M.V. B�¼ttow, M.C.B. Raseira and S. Bonow
Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Pru.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029666
- DOI:
- 10.4238/gmr16029666
Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain
Authors: J.H. Hu1,2*, F.Y. Zhang1*, K.J. Jiang1, Y.B. Fang1,2, J. Wang1,2, M. Zhao1,2, Z.G. Qiao1 and L.B. Ma1
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.19
- DOI:
- 10.4238/2014.December.4.19
Influence of aberrant myeloid expression on acute lymphoblastic leukemia in children and adolescents from Maranh�£o, Brazil
Authors: T.C. Lopes1, K.N.S. Andrade1, N.L. Camelo1, V.P. Rodrigues2 and R.A.G. Oliveira3
The aim of this study was to evaluate myeloid expression in acute lymphoblastic leukemia (ALL) in children and adolescents who had been referred to the Oncology Department in a hospital in the State of Maranhão based on demographic, laboratory, and clinical data. Myeloid expression was evaluated in 65 patients under 18 years of age who were diagnos.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.25
- DOI:
- 10.4238/2014.December.4.25
Association between DLK1 and IGF-I gene expression and meat quality in sheep
Authors: R. Su1, W. Sun1, D. Li1, Q.Z. Wang1, X.Y. Lv1, H.H. Musa2, L. Chen3, Y.F. Zhang3 and W.Z. Wu3
The aim of the present study was to detect delta-like 1 homolog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality... Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.26
- DOI:
- 10.4238/2014.December.4.26
Expression of calpastatin and myostatin genes associated with lamb meat quality
Authors: A. Bagatoli1, E. Gasparino1, M.A.M. Soares2, R.M. Amaral1,F.A.F. Macedo1, D.M. Voltolini1 and A.P. Del Vesco1
Calpastatin (CAST) is an endogenous calpain inhibitor and its main function is to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration. The myostatin gene (GDF-8) acts as a negative regulator of skeletal muscle growth. The expression of these two genes, as well as their interaction, affects the quality of the meat.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.3
- DOI:
- 10.4238/2013.December.4.3
Functions of microRNA in response to cocaine stimulation
Authors: L.-F. Xu, J. Wang, F.B. Lv and Q. Song
MicroRNAs (miRNAs) are a type of non-protein-coding single-stranded RNA, which are typically 20-25 nt in length. miRNAs play important roles in various biological processes, including development, cell proliferation, differentiation, and apoptosis. We aimed to detect the miRNA response to cocaine stimulations and their target .. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.2
- DOI:
- 10.4238/2013.December.4.2
Cloning and expression analysis of the 37-kDa laminin receptor precursor gene from Hyriopsis cumingii
Authors: X.Z. Chang1, J.L. Li1,2, Z.Y. Bai1 and X.L. Li1
Hyriopsis cumingii is an economically important freshwater pearl mussel with high pearl quality that is endemic in China. Investigation of genes relevant to shell formation is important for increased pearl output. The substances that form mollusk shells are secreted by epithelial cells in the mantle, the proliferation of which influences secretion ability.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.2.10
- DOI:
- 10.4238/2013.December.2.10