Expression
Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility
Authors: Z.C. Wu, Y. Liu, W.H. Dong, S.Y. Sun, G.Q. Zhu, S.L. Wu and W.B. Bao
The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expres.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.15
- DOI:
- 10.4238/2015.February.6.15
Tetrandrine induces microRNA differential expression in human hypertrophic scar fibroblasts in vitro
Authors: Tetrandrine induces microRNA differential expression in human hypertrophic scar fibroblasts in vitro
MicroRNAs (miRNAs) have recently been shown to play a role in normal wound healing process. miRNAs may be linked to pathologic wound healing and closely related to the formation of hypertrophic scars. This study aimed to explore the effects of tetrandrine on the miRNA expression profile in human hypertrophic scar fibroblasts (HSFs) in vitro. HSFs were ran.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017288
- DOI:
- 10.4238/gmr.15017288
Selection and validation of reference houseÃ?Âkeeping genes in the J774A1 macrophage cell line for quantitative real-time PCR
Authors: F.B. Ferraz and J.H. Fernandez
Macrophages are essential components of the innate and adaptive immune responses, playing a decisive role in atherosclerosis, asthma, obesity, and cancer. The differential gene expression resulting from adhesion of macrophages to the extra-cellular matrix (ECM) has been studied in the J774A1 murine macrophage cell line using quantitative polymerase chain .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017720
- DOI:
- 10.4238/gmr.15017720
Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi
Authors: N. Ally X.L. Zou B.C. Jiang L. Qin L. Zhai P. Xiao H.L. Liu
Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonuc.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.28.1
- DOI:
- 10.4238/2012.November.28.1
Recombinant expression and characterization of a cysteine peptidase from Xanthomonas citri subsp citri
Authors: A. Soares-Costa R.S. Silveira M.T.M. Novo M.F.M. Alves A.K. Carmona J. Belasque Jr. F. Henrique-Silva
Xanthomonas citri subsp citri (Xac) is the bacterium responsible for citrus canker disease in citrus plants. The aim of this study was to describe the recombinant expression, purification, and characterization of a cysteine peptidase from Xac strain 306, which is a candidate for involvement in the pathogenicity of this bacterium. The gene was cloned and expr.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.28.3
- DOI:
- 10.4238/2012.November.28.3
Identification of transgenic cloned dairy goats harboring human lactoferrin and methylation status of the imprinted gene IGF2R in their lungs
Authors: Y.L. Zhang, G.M. Zhang, Y.J. Wan, R.X. Jia, P.Z. Li, L. Han, F. Wang and M.R. Huang
Dairy goat is a good model for production of transgenic proteins in milk using somatic cell nuclear transfer (SCNT). However, animals produced from SCNT are often associated with lung deficiencies. We recently produced six transgenic cloned dairy goats harboring the human lactoferrin gene, including three live transgenic clones and three deceased transgen.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.3
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.3
Transcriptome analysis of Arabidopsis seedlings responses to high concentrations of glucose
Authors: L. Han, J.L. Li, M. Jin and Y.H. Su
Sugars acting as fuel energy or as signaling molecules play important roles in plant growth and development. Although sugars associated with early seedling development have been analyzed in detail, few studies have examined the effect of sugar on genome-wide gene transcription. To analyze the role of glucose on the genomic level, we examined the response .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.11
- DOI:
- 10.4238/2015.May.11.11
Cloning and expression of ethylene receptor ERS1 at various developmental and ripening stages of mango fruit
Authors: C.A. Contreras-Vergara N.A. Stephens-Camacho G. Yepiz-Plascencia G.A. Gonz�¡lez-Aguilar A.A. Arvizu-Flores E. Sanchez-Sanchez M.A. Islas-Osuna
Ethylene induces characteristic ripening reactions in climacteric fruits through its binding to histidine-kinase (HK) receptors, activating the expression of ripening genes. Ethylene receptors have been found in Arabidopsis thaliana (Brassicaceae) and some fruits; number and expression patterns differ among species. In mango, only ethylene receptor ETR1 was .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.10.6
- DOI:
- 10.4238/2012.September.10.6
FAS ligand expression in inflammatory infiltrate lymphoid cells as a prognostic marker in oral squamous cell carcinoma
Authors: G.T. Peterle, M. Santos, S.O. Mendes, P.B. Carvalho-Neto, L.L. Maia, E. Stur, L.P. Agostini, C.V.M. Silva, L.O. Trivilin, F.D. Nunes, M.B. Carvalho, E.H. Tajara, I.D. Louro and A.M.A. Silva-Conforti
Currently, the most important prognostic factor in oral squamous cell carcinoma (OSCC) is the presence of regional lymph node metastases, which correlates with a 50% reduction in life expectancy. We have previously observed that expression of hypoxia genes in the tumor inflammatory infiltrate is statistically related to prognosis in OSCC. FAS and FASL exp.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.8
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.8
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions
Authors: T.J. Nakayama, F.A. Rodrigues, N. Neumaier, F.C. Marcelino-Guimar�£es, J.R.B. Farias, M.C.N. de Oliveira, A. Bor�©m, A.C.B. de Oliveira, B.M. Emygdio and A.L. Nepomuceno
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantifi cation is an effective strategy .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.13.4
- DOI:
- 10.4238/2014.February.13.4