Expression
Cloning, molecular characterization, and expression pattern of FGF5 in Cashmere goat (Capra hircus)
Authors: W.L. Bao, R.Y. Yao, Q. He, Z.X. Guo, C. Bao, Y.F. Wang and Z.G. Wang
Fibroblast growth factor 5 (FGF5) is a secreted signaling protein that belongs to the FGF family, and was found to be associated with hair growth in humans and other animals. The Inner Mongolia Cashmere goat (Capra hircus) is a goat breed that provides superior cashmere; this breed was formed by spontaneous mutation in China. Here, we report the cloning, .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.9
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.9
Identification and analysis of the jnk1 gene in polyploid hybrids of red crucian carp (Carassius auratus red var.) and common carp (Cyprinus carpio L.)
Authors: Y.M. Xiao, M.G. Jiang, Z.W. Luo, Y.H. Zhou, S. Wen, M. Wang,C. Zhang and S.J. Liu
c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase superfamily. The allotetraploid crucian carp is a product of distant hybridization of female red crucian carp with male common carp. It is the first natural case of an allotetraploid with stable genetic characters, including fertility .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.1
- DOI:
- 10.4238/2014.February.19.1
Low miR-29c expression is a prognostic marker in hepatocellular carcinoma
Authors: C.W. Dong, Y.X. Wang, F.T. Du, W. Ding and S.Y. Hu
A previous study has revealed that miR-29c functions as a tumor suppressor in hepatocellular carcinoma (HCC), but the clinical significance and prognostic value of miR-29c in HCC have not been investigated. Paired human HCC tissues and adjacent noncancerous tissues were obtained from 91 patients, between 2008 to 2014. Quantitative real-time PCR (qRT-PCR) .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037316
- DOI:
- 10.4238/gmr.15037316
Aluminum triggers broad changes in microRNA expression in rice roots
Authors: J.C. Lima, R.A. Arenhart, M. Margis-Pinheiro and R. Margis
MicroRNAs are small 21-nucleotide RNA molecules with regulatory roles in development and in response to stress. Expression of some plant miRNAs has been specifically associated with responses to abiotic stresses caused by cold, light, iron, and copper ions. In acid soils, aluminum solubility increases, thereby causing severe d.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.10.4
- DOI:
- 10.4238/2011.November.10.4
Identification and expression analysis of the MSP130-related-2 gene from Hyriopsis cumingii
Authors: G.-L. Wang, X.-L. Xia, X.-L. Li, F.-H. He and J.-L. Li
MSP130-related-2 is thought to play a role in biomineralization as revealed in Crassostrea gigas and sea urchins. In this study, an MSP130-related-2 gene was isolated from Hyriopsis cumingii (HcMSP130-related-2) and characterized for the first time. The HcMSP130-related-2 cDNA was 2307 bp in length and consisted of a 572-bp 5'-untranslated region (5'-UTR).. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.11.23
- DOI:
- 10.4238/2015.May.11.23
Establishing references for gene expression analyses by RT-qPCR in Theobroma cacao tissues
Authors: T.T. Pinheiro, C.G. Litholdo Jr., M.L. Sereno, G.A. Leal Jr., P.S.B. Albuquerque and A. Figueira
Lack of continuous progress in Theobroma cacao (Malvaceae) breeding, especially associated with seed quality traits, requires more efficient selection methods based on genomic information. Reverse transcript quantitative PCR (RT-qPCR) has become the method of choice for gene expression analysis, but relative expression analysi.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.17.4
- DOI:
- 10.4238/2011.November.17.4
Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis
Authors: Z.T. Ding, C. Li, H. Shi, H. Wang and Y. Wang
CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied b.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.20
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.20
Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata)
Authors: S.J. Liu, F.F. Chen, Y. Dai, C. Wu, Q.S. Ni and W.Y. Yu
The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of.. Read More»
- Genet. Mol. Res. 10(4):
Semi-quantitative detection of gene expression using bisbenzimide dye
Authors: P. Kittimongkolsuk, T. Tencomnao, R. Santiyanont
An electrochemical biosensor, using a disposable electrochemical printed chip aggregation by the bisbenzimide dye (Hoechst 33258), was used for detecting the expression of b-actin and RAGE genes. Using linear sweep voltammetry, the expression of these two genes in HeLa and HepG2 cell lines was determined based on anodic peak c.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.22.8
- DOI:
- 10.4238/2011.November.22.8
Cloning and expression pattern analysis of BkGRAS2 from Betula kirghisorum
Authors: G.Y. Li, C.J. Yang and G.J. Liu
GRAS proteins are plant-specific transcription factors that are involved in the regulation of root and shoot growth. Here, we cloned BkGRAS2 from Betula kirghisorum (abbreviated to Bk) and analyzed the physicochemical properties and expression pattern of the encoded protein. BkGRAS2 had an open reading frame of 1614 bp encoding 537 amino acid residues. Th.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.27
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.27