Expression
Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots
Authors: C.R. de Souza F.J. Arag�£o E.C.O. Moreira C.N.M. Costa S.B. Nascimento L.J. Carvalho
Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be neces.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr560
- DOI:
- 10.4238/vol8-1gmr560
Codon optimization enhances the expression of porcine �²-defensin-2 in Escherichia coli
Authors: C.Y. Gao, T.T. Xu, Q.J. Zhao and C.L. Li
Porcine β-defensin-2 (pBD2) is a cationic antimicrobial peptide that has therapeutic potential. The amount of pBD2 in nature is limited, and the expression of pBD2 in Escherichia coli is low, probably because a different gene codon is used by prokaryotic organisms to that used by eukaryotes. Codon preference optimization is one of the ways to increas.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.12.1
- DOI:
- 10.4238/2015.May.12.1
Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca)
Authors: B. Song, Y.L. Hou, X. Ding, T. Wang, F. Wang, J.C. Zhong, T. Xu, J. Zhong, W.R. Hou and S.R. Shuai
Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymer.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.20.1
- DOI:
- 10.4238/2014.February.20.1
Long-term effects of evodiamine on expressions of lipogenesis and lipolysis genes in mouse adipose and liver tissues
Authors: D.F. Jiang, W.T. Li2, H.L. Yang, Z.Z. Zhang, D. Chen and C. Sun
Evodiamine, the major alkaloid component isolated from the fruit of dried, unripened Evodia rutaecarpa Bentham, affects the plasma levels of cholecystokinin and various biological events such as gastric emptying and gastrointestinal transit; these effects of evodiamine were previously investigated in male rats. In this study, .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.20.5
- DOI:
- 10.4238/2014.February.20.5
IFGFA: Identification of featured genes from genomic data using factor analysis
Authors: C.H. Fu, S. Deng, J.H. Wu, X.Q. Wu, Z.H. Fu and Z.G. Yu
In this study, a software tool (IFGFA) for identification of featured genes from gene expression data based on latent factor analysis was developed. Despite the availability of computational methods and statistical models appropriate for analyzing special genomic data, IFGFA provides a platform for predicting colon cancer-related genes and can be applied .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038803
- DOI:
- 10.4238/gmr.15038803
Meta-analysis of differentially expressed genes in ankylosing spondylitis
Authors: Y.H. Lee and G.G. Song
The purpose of this study was to identify differentially expressed (DE) genes and biological processes associated with changes in gene expression in ankylosing spondylitis (AS). We performed a metaanalysis using the integrative meta-analysis of expression data program on publicly available microarray AS Gene Expression Omnibus (GEO) datasets. We performed.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.6
- DOI:
- 10.4238/2015.May.18.6
Genome-wide analysis of the maize (Zea may L.) CPP-like gene family and expression profiling under abiotic stress
Authors: X.Y. Song, Y.Y. Zhang, F.C. Wu and L. Zhang
Cysteine-rich polycomb-like (CPP) proteins are members of a small family of transcription factors, which have been identified and characterized in Arabidopsis, rice, and soybean. In this study, we investigated CPP-like genes in the maize genome. The results revealed 13 putative CPP-like genes, which were found to encode 17 distinct transcripts and were di.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038023
- DOI:
- 10.4238/gmr.15038023
Expression profiles of genes associated with mitochondria-mediated apoptosis and their roles in liver regeneration
Authors: X.K. Xing, M.H. Li, Z.W. Guo and C.S. Xu
Mitochondria are closely associated with cell survival, and it is of interest to determine whether apoptosis pathways, which are mediated by mitochondria, are involved in liver regeneration (LR). To identify the mechanisms underlying mitochondria-mediated apoptosis during rat LR, we used the Rat Genome 230 2.0 Array to investigate changes in gene expressi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038101
- DOI:
- 10.4238/gmr.15038101
Isolation and characterization of the papaya MADS-box E-class genes, CpMADS1 and CpMADS3, and a TM6 lineage gene CpMADS2
Authors: M.-J. Lee, W.-J. Yang, C.-T. Chiu, J.-J. Chen, F.-C. Chen and L.-S. Chang
Papaya (Carica papaya L.) plants are polygamous, with female, male, and hermaphroditic flowers. To understand the roles of MADS-box genes in flower development and sex determination, we cloned cDNAs of E-class genes CpMADS1 and CpMADS3 and a TM6 lineage of the B-class gene CpMADS2 from young.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.9
- DOI:
- 10.4238/2014.July.24.9
Expression of the porcine lipoic acid synthase (LIAS) gene in Escherichia coli
Authors: W.F. Lu, J.J. Cao, Y.J. Guo, K. Zhong, G.M. Zha, L.F. Wang and G.Y. Yang
Lipoic acid synthase, which exists primarily in mitochondria, participates in the biosynthesis of intrinsic lipoic acid. The lipoic acid synthase gene in pig is known as LIAS. To further investigate the biological functions of the protein that is encoded by LIAS, we cloned the open read frame of porcine LIAS (GenBank No. JN797612.1) into.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.24.16
- DOI:
- 10.4238/2014.July.24.16