Expression
Molecular cloning, expression and variation analyses of the dopamine D2 receptor gene in pig breeds in China
Authors: H.P. Xu, X.M. He, M.X. Fang, Y.S. Hu, X.Z. Jia, Q.H. Nie and X.Q. Zhang
The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou,.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.5.6
- DOI:
- 10.4238/2011.December.5.6
Gene expression, serum amino acid levels, and growth performance of pigs fed dietary leucine and lysine at different ratios
Authors: H. GarcÃ?Âa, A. Morales, A. Araiza, J.K. Htoo and M. Cervantes
We examined 96 pigs (28.1 ± 0.83 kg) to analyze the effect of Leu:Lys ratios on expression of the cationic amino acid transporters b0,+ and CAT-1 in the jejunum and liver as well as myosin expression in 2 muscles to estimate the optimum standardized ileal digestible (SID) Leu:Lys ratio for growth rate and efficiency. A wheat-and wheat bran-based di.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.6
- DOI:
- 10.4238/2015.March.6.6
Correlation between liver cancer occurrence and gene expression profiles in rat liver tissue
Authors: C.S. Xu, G.P. Wang, L.X. Zhang, C.F. Chang, J. Zhi and Y.P. Hao
Liver cancer (LC) is generally characterized by malignant cell proliferation and growth; it normally develops in stages that progress from non-specific injury of the liver to liver fibrosis, liver cirrhosis, dysplasia nodules, and liver carcinoma. We used a rat model of diethylnitrosamine (DENA)-induced LC; a Rat Genome 230 2... Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.14.3
- DOI:
- 10.4238/2011.December.14.3
Gene expression profile of the plant pathogen Fusarium graminearum under the antagonistic effect of Pantoea agglomerans
Authors: V. Pandolfi, E.C. Jorge, C.M.R. Melo, A.C.S. Albuquerque and H. Carrer
The pathogenic fungus Fusarium graminearum is an ongoing threat to agriculture, causing losses in grain yield and quality in diverse crops. Substantial progress has been made in the identification of genes involved in the suppression of phytopathogens by antagonistic microorganisms; however, limited information regarding respo.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr828
- DOI:
- 10.4238/vol9-3gmr828
Molecular, anatomical and physiological properties of a genetically modified soybean line transformed with rd29A:AtDREB1A for the improvement of drought tolerance
Authors: A.M. Polizel, M.E. Medri, K. Nakashima, N. Yamanaka, J.R.B. Farias, M.C.N. de Oliveira, S.R.R. Marin, R.V. Abdelnoor, F.C. Marcelino-Guimar�£es, R. Fuganti, F.A. Rodrigues, R. Stolf-Moreira, M.A. Beneventi, A.A.P. Rolla, N. Neumaier, K. Yamaguchi-Shinozaki, J.F.C. Carvalho and A.L. Nepomuceno
We evaluated the molecular, anatomical and physiological properties of a soybean line transformed to improve drought tolerance with an rd29A:AtDREB1A construct. This construct expressed dehydration- responsive element binding protein DREB1A from the stress-inducible rd29A promoter. The greenhouse growth test included four rand.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.21.4
- DOI:
- 10.4238/2011.October.21.4
Transcription factors expressed in soybean roots under drought stress
Authors: S.S. Pereira, F.C.M. Guimar�£es, J.F.C. Carvalho, R. Stolf-Moreira, M.C.N. Oliveira, A.A.P. Rolla, J.R.B. Farias, N. Neumaier and A.L. Nepomuceno
To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcript.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.21.5
- DOI:
- 10.4238/2011.October.21.5
Combining P values to improve classification of differential gene expression in the HTself software
Authors: D.A. Cortez, A.P. Tonon, P. Colepicolo and R.Z.N. V�ªncio
HTself is a web-based bioinformatics tool designed to deal with the classification of differential gene expression in low replication microarray studies. It is based on a statistical test that uses self-self experiments to derive intensity-dependent cutoffs. We developed an extension of HTself, originally released in 2005, by .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.5.5
- DOI:
- 10.4238/2011.December.5.5
Genomic structure, polymorphism and expression analysis of growth hormone-releasing hormone and pituitary adenylate cyclase activating polypeptide genes in the half-smooth tongue sole (Cynoglossus semilaevis)
Authors: Q. Ma, S.F. Liu, Z.M. Zhuang, L. Lin, Z.Z. Sun, C.L. Liu, Y.Q. Su and Q.S. Tang
Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase activating polypeptide (PACAP) regulate development and somatic growth in teleosts; they may be associated with sexual growth dimorphism in the half-smooth tongue sole (Cynoglossus semilaevis). We found that the full-length GHRH and PACAP gene sequences ob.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.12.1
- DOI:
- 10.4238/2011.December.12.1
Identification of critical TF-miRNA-mRNA regulation loops for colorectal cancer metastasis.
Authors: C. Wang, D.Z. Hu and J.Z. Liu
To explore the potential cause of colorectal cancer metastasis, gene expression profiles, GSE21510, and miRNA expression profiles, GSE48074, were downloaded from the Gene Expression Omnibus database. Differentially expressed genes in metastatic colorectal and non metastatic colorectal cancer compared with the normal samples were identified via the limma p.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.19
- DOI:
- 10.4238/2015.May.22.19
Characterization and expression of the calpastatin gene in Cyprinus carpio
Authors: W.X. Chen and Y. Ma
Calpastatin, an important protein used to regulate meat quality traits in animals, is encoded by the CAST gene. The aim of the present study was to clone the cDNA sequence of the CAST gene and detect the expression of CAST in the tissues of Cyprinus carpio. The cDNA of the C. carpio CAST gene, amplified using rapid amplification of cDNA ends PCR, is 2834 .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.16
- DOI:
- 10.4238/2015.July.3.16