Expression
Identification of critical TF-miRNA-mRNA regulation loops for colorectal cancer metastasis
Authors: C. Wang D.Z. Hu and J.Z. Liu
To explore the potential cause of colorectal cancer metastasis, gene expression profiles, GSE21510, and miRNA expression profiles, GSE48074, were downloaded from the Gene Expression Omnibus database. Differentially expressed genes in metastatic colorectal and non metastatic colorectal cancer compared with the normal samples were identified via the limma p.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.19
- DOI:
- 10.4238/2015.May.22.19
Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing
Authors: H. Shen, H.Y. Chen, B. Jia, G.H. Han, Y.S. Zhang and X.C. Zeng
The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understandi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.11
- DOI:
- 10.4238/2015.July.3.11
Temporal and tissue expression of genes involved in buds of earliness cotton cultivar
Authors: V.G.L. Batista, M.P.N. Pinheiro, P.A. Melo Filho, R.C. Santos and L.M. Lima
Gene sequences previously identified in Arabidopsis buds were used as references in order to estimate temporal and tissue expression in buds, leaves, stem, and root tissues in cotton plants. Buds were evaluated during 3 phases: 2-8, 10-12, and 14-20 mm. Primers were designed for the ARF6, ATFY, and SEUSS genes for use in semi-quantitative reverse transcri.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.14
- DOI:
- 10.4238/2015.July.3.14
Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li, H.G. Feng, J.T. Lin and Z.Q. Yuan
We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-NGF-VEGF165. The nerve growth factor (NGF) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NGF cDNA fragment was inserted into the multiple cloning sites of the pI.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.23
- DOI:
- 10.4238/2014.July.25.23
Transcriptomic identification of chemoreceptor genes in the red palm weevil Rhynchophorus ferrugineus
Authors: W. Yan, L. Liu, W.Q. Qin, C.X. Li and Z.Q. Peng
Olfaction is crucial for insects’ survival because it enables them to recognize various environmental information. It is primarily mediated by a large family of chemoreceptors, including olfactory receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs). Here, we assembled the transcriptome of the economically important pest of pal.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.23
- DOI:
- 10.4238/2015.July.3.23
Analysis of skin color change and related gene expression after crossing of Dongxiang black chicken and ISA layer
Authors: X.D. Zhang, H.H. Wang, C.X. Zhang, Q.H. Li, X.H. Chen and L.F. Lou
This study evaluated the effects of the autosomal dominant Fm gene in conjunction with the sex-linked Id gene on skin color and related gene expression. Ten Dongxiang black cocks were selected to build ten families by mating 60 individuals of ISA B-line layers. The skin color of the F1 generation was observed at different time points. At 126 days, 36 chic.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.7
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.7
Quantitative changes in mitochondrial DNA copy number in various tissues of pigs during growth
Authors: Y.M. Xie, L. Jin, X.J. Chen, M.N. He, Y. Wang, R. Liu, M.Z. Li and X.W. Li
Mitochondrial DNA (mtDNA) content is dependent on the energy requirements of tissues. To date, no comprehensive study has been conducted to examine mtDNA copy number variations in pigs. In the current study, quantitative polymerase chain reaction was performed to quantify the mtDNA copy number in 15 pig tissue types at 5 growth stages from embryo to adult.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.12
- DOI:
- 10.4238/2015.March.6.12
Influence of sugars and hormones on the genes involved in sucrose metabolism in maize endosperms
Authors: X.D. Ren, H.M. Liu, Y.H. Liu, Y.F. Hu, J.J. Zhang and Y.B. Huang
Starch is the major storage product in the endosperm of cereals. Its synthesis is closely related to sucrose metabolism. In our previous study, we found that the expression of most of the genes involved in starch synthesis might be regulated by sugars and hormones in the maize endosperm. However, little is known regarding the transcriptional regulation of.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.13
- DOI:
- 10.4238/2015.March.6.13
Hypoxia-related gene expression in porcine skeletal muscle tissues at different altitude
Authors: J. Zhang, L. Chen, K.R. Long and Z.P. Mu
Hypoxia influences many physiological processes, such as respiration, cardiovascular, neurophysiology, and digestion. Skeletal muscle is an important motor organ, which relies on oxygen of oxidation; however, the study of hypoxia in skeletal muscle is lacking. In order to understand the effect of hypoxia on skeletal muscle, we determined the expression le.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.10
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.10
Expression and hypoxia adaptation analysis of the EPO gene in different tissues of plateau Tibetan pigs
Authors: B.Z. Deji, P. Shang, W.J. Danzeng, H. Zhang and Y.Z. Qiangba
This study aimed to observe the expression characteristics of the erythropoietin (EPO) gene in different tissues of Tibetan pigs and to explore the adaptation to hypoxic environments. The cDNA in heart, liver, lung, kidney, muscle, brain, and fat of Tibetan pigs was used as the template. Through the number of cycles of the polymerase chain reaction (PCR),.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.6.16
- DOI:
- 10.4238/2015.March.6.16