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Expression

Animal Genetics   Research Article

Morphological analysis and muscle-associated gene expression during different muscle growth phases of Megalobrama amblycephala

Authors: K.C. Zhu, D.H. Yu, J.K. Zhao, W.M. Wang and H.L. Wang

Skeletal muscle growth is regulated by both positive and negative factors, such as myogenic regulatory factors (MRFs) and myostatin (MSTN), and involves both hyperplasia and hypertrophy. In the present study, morphological changes during muscle development in Megalobrama amblycephala were characterized and gene expression levels were measured by quantitat.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.28.16
DOI:
http://dx.doi.org/10.4238/2015.September.28.16
Microbial Genetics   Research Article

Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers

Authors: Z. Jastrz��bski and M. �»ychowska

The aim of this study was to demonstrate the effects of 6-week low-intensity training on changes in indicators of aerobic capacity and on HSPA1A, HSPB1, and LDHb expression in white blood cells in high level rowers. We hypothesized that the type of training would have an impact not only on the adaptation of athletes to the aerobic nature of the exercises,.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.29
DOI:
10.4238/2015.July.3.29
Animal Genetics   Research Article

Cloning and expression analysis of a heat shock protein 90 �² isoform gene from the gills of Wuchang bream (Megalobrama amblycephala Yih) subjected to nitrite stress

Authors: S.M. Sun, J. Zhu, X.P. Ge, C.F. Zhang, L.H. Miao and X.J. Jiang

Heat shock protein 90 (HSP90), a highly conserved and multi-functional molecular chaperone, plays an essential role in cellular metabolism and stress response. In this study, HSP90 cDNA named MaHSP90 was cloned from Wuchang bream (Megalobrama amblycephala) gills by using rapid amplification of cDNA ends. The full-length MaHSP90 cDNA is 2674 bp and consist.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.10.14
DOI:
http://dx.doi.org/10.4238/2015.April.10.14
Animal Genetics   Research Article

Expression patterns and promoter activity analysis of UGP2 in pigs.

Authors: J.Y. Yu, S.M. Shao, K. Chen, M.G. Lei, and Y.Z. Xiong,

The function of the UDP-glucose pyrophosphorylase 2 gene (UGP2) in pig is not clear. In the present study, we used RNA isolated from Large White pigs and Chinese indigenous MeiShan pigs to examine the temporal coordination of changes in gene expression within muscle tissues. We cloned both the complete genomic DNA sequence and.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.February.28.8
DOI:
http://dx.doi.org/10.4238/2014.February.28.8
Plant Genetics   Research Article

Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

Authors: D.M. Li, F.B. LÃ?Â?, G.F. Zhu, Y.B. Sun, H.L. Liu, J.W. Liu and Z. Wang

Warm day and cool night conditions significantly induce reproductive spike formation in Phalaenopsis plants; hence, determining the flowering mechanism regulating the reproductive transition is important. Flowering locus T (FT) plays important roles in flowering induction in several plants. To explore spike i.. Read More»

Genet. Mol. Res. 13(3):
2014.August.7.14
DOI:
10.4238/2014.August.7.14
Animal Genetics   Research Article

Antibody study in canine distemper virus nucleocapsid protein gene-immunized mice

Authors: B. Yuan, X.Y. Li, T. Zhu, L. Yuan, J.P. Hu, J. Chen, W. Gao and W.Z. Ren

The gene for the nucleocapsid (N) protein of canine distemper virus was cloned into the pMD-18T vector, and positive recombinant plasmids were obtained by enzyme digestion and sequencing. After digestion by both EcoRI and KpnI, the plasmid was directionally cloned into the eukaryotic expression vector pcDNA; the positive clone pcDNA-N was screened by elec.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.10.20
DOI:
http://dx.doi.org/10.4238/2015.April.10.20
Microbial Genetics   Research Article

Branches of NF-�ºb signaling pathway regulate hepatocyte proliferation in rat liver regeneration

Authors: C.F. Chang, W.M. Zhao, J.X. Mei, Y. Zhou, C.Y. Pan, T.T. Xu and C.S. Xu

Previous studies have demonstrated that the nuclear factor κB (NF-κB) pathway is involved in promoting cell proliferation. To further explore the regulatory branches and their sequence in the NF-κB pathway in the promotion of hepatocyte proliferation at the transcriptional level during rat liver regeneration, Rat Genome 230 2.0 array was.. Read More»

Genet. Mol. Res. 14(3):
2015.July.13.9
DOI:
10.4238/2015.July.13.9
Plant Genetics   Research Article

Expression profile analysis of ascorbic acid-related genes in response to temperature stress in the tea plant, Camellia sinensis (L.) O. Kuntze

Authors: H. Li, W. Huang, G.L. Wang, Z.J. Wu and J. Zhuang, Tea Scienc

Ascorbic acid (AsA), also known as ascorbate or vitamin C, is a natural organic compound in green plants that has antioxidant properties, and is an essential nutrient for humans. The tea plant, Camellia sinensis (L.) O. Kuntze, is an important global economic crop. Here, the expression profiles of genes related to AsA biosynth.. Read More»

Genet. Mol. Res. 15(4):
gmr.15048756
DOI:
10.4238/gmr.15048756
Plant Genetics   Research Article

Genetic mapping of Theobroma cacao (Malvaceae) seedlings of the Parinari series, carriers of the lethal gene Luteus-Pa

Authors: B.C. Rehem, A.-A.F. Almeida, R.X. Corr�ªa, A.S. Gesteira, M.M. Yamada and R.R. Valle

The lethal gene ‘Luteus-Pa’ is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of ‘Luteus-Pa&rsquo.. Read More»

Genet. Mol. Res. 9(3):
vol9-3gmr889
DOI:
10.4238/vol9-3gmr889
Animal Genetics   Research Article

Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense

Authors: Y.P. Zhang, S.M. Sun, H.T. Fu, X.P. Ge, H. Qiao, W.Y. Zhang, Y.W. Xiong, S.F. Jiang, Y.S. Gong and S.B. Jin

In this study, male-specific lethal 3 homolog (Mnmsl3) was cloned and characterized from the freshwater prawn Macrobrachium nipponense (Crustacea: Decapoda: Palaemonidae) by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnmsl3 showed high-sequence homology to the insect Msl3 and contained a conserved chromatin organization modifier.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.10.21
DOI:
http://dx.doi.org/10.4238/2015.April.10.21