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Expression

Plant Genetics   Research Article

Cloning and quantitative expression analysis of drought-induced genes in soybean

Authors: R. Stolf-Moreira, M.E. Medri, N. Neumaier, N.G. Lemos, R.L. Brogin, F.C. Marcelino, M.C.N. de Oliveira, J.R.B. Farias, R.V. Abdelnoor and A.L. Nepomuceno

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought .. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr701
DOI:
10.4238/vol9-2gmr701
Plant Genetics   Research Article

Genome-wide identification, classification, and analysis of heat shock transcription factor family in Chinese cabbage (Brassica rapa pekinensis)

Authors: X.Y. Huang, P. Tao, B.Y. Li, W.H. Wang, Z.C. Yue, J.L. Lei andX.M. Zhong

Chinese cabbage (Brassica rapa ssp. pekinensis) is one of the most important vegetable crops grown worldwide, and various methods exist for selection, propagation, and cultivation. The entire Chinese cabbage genome has been sequenced, and the heat shock transcription factor family (Hsfs) has been found to play a central role in plant growth and developmen.. Read More»

Genet. Mol. Res. 14(1):
2015.March.27.5
DOI:
10.4238/2015.March.27.5
Human Genetics   Research Article

Abnormal gene expression profile reveals the common key signatures associated with clear cell renal cell carcinoma: a meta-analysis

Authors: H.J. Zhang, Z.Q. Sun, W.Q. Qian and L. Sheng

The aims of this study were to identify the common gene signatures of clear cell renal cell carcinoma (CCRCC), and to expand the respective protein-protein interaction networks associated with CCRCC regulation. For the latter, we utilized multiple gene expression data sets from the National Center for Biotechnology Information (NCBI) Gene Expression Omnib.. Read More»

Genet. Mol. Res. 14(1):
2015.March.27.7
DOI:
10.4238/2015.March.27.7
Human Genetics   Research Article

A C-type lectin fold gene from Japanese scallop Mizuhopecten yessoensis, involved with immunity and metamorphosis

Authors: X.B. Bao, C.B. He, C.D. Fu, B. Wang, X.M. Zhao, X.G. Gao andW.D. Liu

C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 16.. Read More»

Genet. Mol. Res. 14(1):
2015.March.27.11
DOI:
10.4238/2015.March.27.11
Microbial Genetics   Research Article

Molecular cloning, characterization, and expression of vacuolar-type-H+-ATPase B1 (VHAB1) gene in the gill of Anguilla marmorata

Authors: L. Li, Y.H. Jia, P. Li, S.W. Yin, G.S. Zhang, X.L. Wang, Y.Y. Wang and X.J. Wang

We explored the molecular mechanism of the regulation of vacuolar-type-H+-ATPase B1 (VHAB1) in elvers in the response to salinity. The full-length cDNA of VHAB1 in Anguilla marmorata (designated as AmVHAB1), which was 1741 base pairs (bp) in length, was found to encompass a 1512-bp open reading frame encoding a polypeptide with 503 amino acids (55.9 kDa),.. Read More»

Genet. Mol. Res. 14(3):
2015.July.17.9
DOI:
10.4238/2015.July.17.9
Animal Genetics   Research Article

Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)

Authors: J. Liu, L.D. Qian, J.L. Huo, B.L. Bi, D.L. Li, S.F. Wang,T. Chen, L.J. Li, H.M. Mao and Y.W. Miao

Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcription-polymerase chain reaction. The protein was then subjected to molecular characterizat.. Read More»

Genet. Mol. Res. 14(1):
2015.March.27.14
DOI:
10.4238/2015.March.27.14
Animal Genetics   Research Article

Screening of differentially-expressed genes in the muscles of rabbit breeds with expression profile chip

Authors: W.Z. Wang, T. Li, L.J. Shi, X.R. Yan, Y.L. Pan and X.S. Wu

The molecular mechanism underlying muscle development in rabbits is not well-understood. In the current study, differentially-expressed genes were scanned using an expression profile chip in New Zealand white rabbits (introduced breed) and Fujian yellow rabbits (local breed), and some of the genes were tested using reverse transcription-polymerase chain r.. Read More»

Genet. Mol. Res. 14(3):
2015.July.17.12
DOI:
10.4238/2015.July.17.12
Plant Genetics   Research Article

Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses

Authors: H.H. Liu, Y.G. Wang, S.P. Wang and H.J. Li

In this study, the full-length cDNA encoding allene oxide cyclase (AhAOC) was isolated from peanut (Arachis hypogaea L.). The deduced amino acid sequence of AhAOC showed high homology with other plant AOCs. The transcript of AhAOC was found to be abundantly expressed in roots. Expression analysis demonstrated that AhAOC was induced by abscisic acid, methy.. Read More»

Genet. Mol. Res. 14(1):
2015.March.27.18
DOI:
10.4238/2015.March.27.18
Plant Genetics   Research Article

Using the attract method to identify core pathways in juvenile idiopathic arthritis

Authors: J.S. Li, X.L. Gao, Y.R. Liu and Y. Dong

The aim of this study was to identify core pathways associated with juvenile idiopathic arthritis (JIA) using the attract method. Kyoto Encyclopedia of Genes and Genomes pathways were determined using the GSEA-ANOVA method, based on the gene expression data of JIA. Syn-expression groups within core attractor pathways were identified by hierarchical cluste.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038331
DOI:
10.4238/gmr.15038331
Bioinformatics   Research Article

BayBoots: a model-free Bayesian tool to identify class markers from gene expression data

Authors: Ricardo Z.N. V�ªncio, Diogo F.C. Patr�£o, Cassio S. Baptista, Carlos A.B. Pereira and Bianca Zingales

One of the goals of gene expression experiments is the identification of differentially expressed genes among populations that could be used as markers. For this purpose, we implemented a modelfree Bayesian approach in a user-friendly and freely available web-based tool called BayBoots. In spite of a common misunderstanding th.. Read More»

Genet. Mol. Res. 5(1):