Authors: Gislaine S.P. Pereira, Rodrigo M. Brand�£o, Silvana Giuliatti, Marco A. Zago and Wilson A. Silva Jr.
Serial analysis of gene expression (SAGE) technology produces large sets of interesting genes that are difficult to analyze directly. Bioinformatics tools are needed to interpret the functional information in these gene sets. We present an interactive web-based tool, called Gene Class, which allows functional annotation of SAG.. Read More»
Authors: L.Q. Li, J. Li, Y. Chen, Y.F. Lu and L.M. Lu
Tobacco is an economically important crop, and its potassium content can greatly affect the quality of tobacco leaves. However, the molecular mechanism involved in potassium starvation in tobacco has not been elucidated to date. In this study, Illumina (Solexa) sequencing technology was used to analyze the transcriptome of tobacco seedlings under low-pota.. Read More»
Authors: X.Q. Wang, J. Han, Y. Wen, W.B. Jiang, J.G. Fang, B.B. Zhang and R.J. Ma
In higher plants, the transcription factor MYB10 is an important regulator of anthocyanin biosynthesis. In order to study its role in the development of red coloration in peach leaves, the full-length MYB10 complementary DNA sequence of the red-leaf peach cultivar ‘Tsukuba No. 5’ (Prunus persica f. atropurpurea) wa.. Read More»
Authors: W. Song, M.D. Zhao, K.J. Jiang, F.Y. Zhang, M. Zhao, Y.Y. Meng and L.B. Ma
The matrix Gla (gamma-carboxyglutamic acid-rich) protein (MGP), a vitamin K-dependent and Gla-containing protein, is a calcification inhibitor that mainly functions in tissue calcification and mineralization. In this study, we obtained the complete cDNA sequence of MGP from the spinyhead croaker (Collichthys lucidus), which we.. Read More»
Authors: I.L. Vighi, L.C. Benitez, M.N. do Amaral, P.A. Auler, G.P. Moraes, G.S. Rodrigues, L.C. da Maia, L.S. Pinto and E.J.B. Braga
Different rice (Oryza sativa L.) genotypes were subjected to high salinity and low temperature (150 mM NaCl and 13°C, respectively) for 0, 6, 24, 48, or 72 h. We evaluated the simultaneous expression of the genes OsCATA, OsCATB, and OsCATC, correlated gene expression with enzyme activity, and verified the regulation of the.. Read More»
Authors: Y.F. Xu, X. Liang, Y.R. Chen, Y.F. Li and J.L. Yang
To understand the potential functions of Wnt7b in different developmental stages and adult tissues of the mussel Mytilus coruscus, the Wnt7b gene was cloned using the rapid amplification of cDNA ends technique. The full-length Wnt7b gene was 1910 bp long, with a 1059-bp open reading frame encoding 352 amino acids. The amino ac.. Read More»
Authors: J.J. Wang, X.K. Lu, Z.J. Yin, M. Mu, X.J. Zhao, D.L. Wang, S. Wang, W.L. Fan, L.X. Guo, W.W. Ye and S.X. Yu
Calcineurin B-like protein-interacting protein kinase (CIPK) plays a key regulatory role in the growth, development, and stress resistance of plants by combining with phosphatase B subunit-like protein. In the present study, CIPK genes were identified in the whole genomes of diploid cottons and their sequences were subjected t.. Read More»
Authors: D.M. Li J.H. Wang S.L. Peng G.F. Zhu F.B. LÃ?Â?
NAC proteins, which are plant-specific transcription factors, have been identified to play important roles in plant response to stresses and in plant development. The full-length cDNAs that encode 2 putative NAC proteins, designated as MmATAF1 and MmNAP, respectively, were cloned from Mikania micrantha by rapid amplification of cDNA ends. The full-length cDN.. Read More»
Authors: S.A.P. Pinto and J.M. Ortega
Following sequence alignment, clustering algorithms are among the most utilized techniques in gene expression data analysis. Clustering gene expression patterns allows researchers to determine which gene expression patterns are alike and most likely to participate in the same biological process being investigated. Gene express.. Read More»
Authors: M.S. Lauretto, C.A.B. Pereira and J.M. Stern
Gene clustering is a useful exploratory technique to group together genes with similar expression levels under distinct cell cycle phases or distinct conditions. It helps the biologist to identify potentially meaningful relationships between genes. In this study, we propose a clustering method based on multivariate normal mixt.. Read More»