Expression
Molecular cloning and expression analysis of the ethylene insensitive3 (EIN3) gene in cucumber (Cucumis sativus)
Authors: B.B. Bie, J.S. Pan, H.L. He, X.Q. Yang, J.L. Zhao and R. Cai
The plant gaseous hormone ethylene regulates many aspects of plant growth, development, and responses to the environment. Ethylene insensitive3 (EIN3) is a key transcription factor involved in the ethylene signal transduction pathway. To gain a better understanding of this particular pathway in cucumber, the full-length cDNA encoding EIN3 (designated as C.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.4
- DOI:
- 10.4238/2013.October.7.4
Differential expression and effect of the porcine ANGPTL4 gene on intramuscular fat
Authors: Z.Q. Ren, W.J. Wu, W.H. Liu, R. Zheng, J.L. Li, B. Zuo, D.Q. Xu, F.E. Li, M.G. Lei, D.B. Ni and Y.Z. Xiong
In a previous study, we investigated differences in gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan, and Meishan x Large White pigs. One potential differentially expressed sequence tag from the mRNA differential display was a homolog of the human angiopoietin-like 4 (.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.3
- DOI:
- 10.4238/2014.April.16.3
Screening of the residual normal ovarian tissue adjacent to orthotopic epithelial ovarian carcinomas in nude mice
Authors: G.H. Zhu, S.T. Wang, M.Z. Yao, J.H. Cai, C.Y. Chen, Z.X. Yang, L. Hong and S.Y. Yang
The objective of this study was to explore the feasibility and methods of screening the residual normal ovarian tissue adjacent to orthotopic ovarian carcinomas in nude mice. Human epithelial ovarian cancer cells (OVCAR3) were subcutaneously implanted for a tumor source and ovarian orthotopic transplantation. The cancer tissue.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.6
- DOI:
- 10.4238/2014.April.16.6
Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori
Authors: Q.M. Chen, D.J. Cheng, S.P. Liu, Z.G. Ma, X. Tan and P. Zhao
Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven gene.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.13.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.13.2
Keloid microRNA expression analysis and the influence of miR-199a-5p on the proliferation of keloid fibroblasts
Authors: Z.-Y. Wu, L. Lu, J. Liang, X.-R. Guo, P.H. Zhang and S.-J. Luo
The purpose of this study was to identify microRNAs (miRNAs) involved in keloid formation and determine their influence on the proliferation of keloid fibroblasts (KFs). Eight specimens each of resected keloid tissue and normal skin tissue were collected. miRNAs that are differentially expressed in keloid tissue and normal ski.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.2
- DOI:
- 10.4238/2014.April.14.2
Molecular cloning and expression of the porcine S14R gene in Escherichia coli
Authors: Y.J. Guo1*, G.Z. Liu1*, C.M. Wang1, Y.Y. Wang2, H.J. Li2, K. Zhong2, W.F. Lu2, Y.L. Wang2, G.Y. Yang2
We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragm.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.6
- DOI:
- 10.4238/2013.October.10.6
Developmental changes in IGF-I and MyoG gene expression and their association with meat traits in sheep
Authors: W. Sun, R. Su1, D. Li, H.H. Musa, Y. Kong, J.T. Ding, Y.H. Ma, L. Chen, Y.F. Zhang and W.Z. Wu
In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.6
- DOI:
- 10.4238/2014.April.14.6
Expression and enzymatic activity of phenylalanine ammonia-lyase and p-coumarate 3-hydroxylase in mango (Mangifera indica �Ataulfo�) during ripening
Authors: H. Palafox-Carlos, C.A. Contreras-Vergara, A. Muhlia-Almaz�¡n, M.A. Islas-Osuna and G.A. Gonz�¡lez-Aguilar
Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar ‘Ataulfo’ in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.10
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.10
Critical evaluation of transcription factor Atf2 as a candidate modulator of alcohol preference in mouse and human populations
Authors: L.S. Wang, Y. Jiao, Y. Huang, X.Y. Liu, G. Gibson, B. Bennett,K.M. Hamre, D.W. Li, H.Y. Zhao, J. Gelernter, H.R. Kranzler,L.A. Farrer, L. Lu, Y.J. Wang, W.K. Gu
In prior work, congenic strains carrying the DBA/2Igb (D2) region of chromosome 2 (Chr2) for alcohol preference were bred onto a C57BL/6Ibg (B6) background and as predicted were found to reduce voluntary consumption. Subsequently, interval-specific congenic recombinant strains (ISCRS) were generated and also tested. These ISCR.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.9
- DOI:
- 10.4238/2013.November.26.9
Changes of gene expression profiles across different phases of vascular calcification in rats
Authors: Z.M. Jiang, X.J. Wu, Y. Liu, X.H. Du, S.J. Shen, L.Y. Xu,W.X. Sun
This study investigated the alteration of gene expression profiles in order to gain a deeper understanding into the molecular mechanism involved in different processes of vascular calcification (VC). Sprague Dawley (SD) rats were injected with 300,000 μg/kg vitamin D3 and gavaged with 25 mg/kg nicotine for 8 or 16 weeks to .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.4
- DOI:
- 10.4238/2013.November.26.4