Expression
Cloning and bioinformatic analysis of full-length novel pepper (Capsicum annuum) genes TAF10 and TAF13
Authors: J.F. Wen, J.L. Huo, H.X. Chen, C.H. Ma, H. Jiang, H.S. Zhu, H. Zhou and M.H. Deng
We isolated two TATA-binding protein-associated factor (TAF) genes, TAF10 and TAF13, from pepper (Capsicum annuum). The complete coding sequences were amplified using reverse transcriptase-PCR on the basis of conserved sequence information of eggplant and several other plant species. Nucleotide sequence analysis of these two genes revealed that the pepper.. Read More»
- Genet. Mol. Res. 12(4):
- 2013
- DOI:
- 10.4238/2013
Three branches of phospholipase C signaling pathway promote hepatocyte growth in rat liver regeneration
Authors: G.G. Xu, Z. Geng, X.C. Zhou, Y.G. He, T.T. He, J.X. Mei,Y.J. Yang, Y.Q. Liu and C.S. Xu
In general, the phospholipase C (PLC) signaling pathway is involved in many physiological activities, including cell growth. However, little is known regarding how the PLC signaling pathway participates in regulating hepatocyte (HC) growth during liver regeneration (LR). To further explore the influence of the PLC signaling pathway on HCs at the cellular .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.3
- DOI:
- 10.4238/2015.May.29.3
Molecular cloning and characterization of a novel Y-box gene from Sepiella maindroni
Authors: H.P. Si, C.L.Wang, Y.Y. Zhang, C.K. Mu, P.P. Zhan, R.H. Li and W.W. Song
Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.7
- DOI:
- 10.4238/2015.May.29.7
Predictive value of CK20 in evaluating the efficacy of treatment and prognosis after surgery for colorectal cancer
Authors: W.X. Li, H.W. Xiao, X.Q. Hong and W.X. Niu
specimens and intraperitoneal drainage fluids, CK20 levels were lower in patients with earlier cancer stages than in those at later stages. During postoperative follow-up, serum negative CK20 patients had significantly higher 3-year survival rates than serum positive CK20 patients. All differences were statistically significant (P < 0.05). CK20 levels .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.14
- DOI:
- 10.4238/2015.May.29.14
Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro
Authors: Z.J. Liu, Y.J. Lv, M. Zhang, Z.H. Yue, S. Tang, A. Islam, B. Rehana, E.D. Bao and J. Hartung
We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.29.1
- DOI:
- 10.4238/2012.November.29.1
Inhibitory effect of alternatively spliced RAGEv1 on the expression of NF-kB and TNF-�± in hepatocellular carcinoma cells
Authors: T. Lertwittayapon, T. Tencomnao and R. Santiyanont
Binding of specific ligands to the receptor for advanced glycation end-products (RAGE) can trigger a series of signal transductions, which leads to pathogenesis in many chronic degenerative diseases, including cancer. Alternative splicing of RAGE mRNA has resulted in many variants, including RAGE variant 1 (RAGEv1). This parti.. Read More»
- Genet. Mol. Res. 11(2):
- http://dx.doi.org/2012.June.29.3
- DOI:
- http://dx.doi.org/10.4238/2012.June.29.3
Trans-translation ensures timely initiation of DNA replication and DnaA synthesis in Escherichia coli
Authors: W. Wurihan, W. Wunier, H. Li, L.F. Fan and M. Morigen
The trans-translation pathway, mediated by the transfer messenger RNA (tmRNA; encoded by the ssrA gene) and the SmpB protein (tmRNA-binding protein expressed in Salmonella enterica), which is conserved in bacteria, is required for various cellular processes. A previous study has shown that trans-translation is required to ensu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038407
- DOI:
- 10.4238/gmr.15038407
Toxoplasma gondii: cloning, sequencing, expression, and antigenic characterization of ROP2, GRA5 and GRA7
Authors: M. Igarashi, F. Kano, K. Tamekuni, P.M. Kawasaki, I.T. Navarro,O. Vidotto, M.C. Vidotto, R.Z. Machado and J.L. Garcia
Toxoplasma gondii is an intracellular obligate protozoan,which infects humans and warm-blooded animals. The aim of the present study was to clone the rop2, gra5 and gra7 genes from T. gondii RH strain and to produce recombinant proteins. The rop2, gra5 and gra7 gene fragments produced by polymerase chain reaction were cloned i.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr423
- DOI:
- 10.4238/vol7-2gmr423
Differential expression of E-cadherin gene in human neuroepithelial tumors
Authors: F.J.N. Motta, E.T. Valera, A.K.B. Lucio-Eterovic, R.G.P. Queiroz,L. Neder, C.A. Scrideli, H.R. Machado, C.G. Carlotti-Junior, S.K.N. Marie and L.G. Tone
Cadherins are cell-to-cell adhesion molecules that play an important role in the establishment of adherent-type junctions by mediating calcium-dependent cellular interactions. The CDH1 gene encodes the transmembrane glycoprotein E-cadherin which is important in maintaining homophilic cell-cell adhesion in epithelial tissues. E.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr424
- DOI:
- 10.4238/vol7-2gmr424
Molecular cloning and expression profile of an ATP-binding cassette (ABC) transporter gene from the hemipteran insect Nilaparvata lugens
Authors: W.J. Zha, S.H. Li, L. Zhou, Z.J. Chen, K. Liu, G.C. Yang, G. Hu, G.C. He and A.Q. You
The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. In insects, ABC transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. In this study, the Nilaparvata lugens S.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.30.26
- DOI:
- http://dx.doi.org/10.4238/2015.March.30.26