Expression
Stable transfection and identification of a hair follicle-specific expression vector of IGFBP-5 in goat fetal fibroblasts
Authors: X.J. Wang, H.M. Su, Y. Liang, Y.F. Wang, X.D. Guo, Z.G. Wang and D.J. Liu
The insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the 6 members of the IGFBP family and is involved in the regulation of cell growth, apoptosis, and other IGF-stimulated signaling pathways. To determine the significance of IGFBP-5 in the Inner Mongolia Cashmere goat (Capra hircus), a hair follicle-specific expression vector of IGFBP-5, .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.16
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.16
Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology
Authors: S. Honsawek, P. Bumrungpanichthaworn, T. Thitiset and L. Wolfinbarger Jr.
Demineralized bone matrix (DBM) has been widely investigated as a biomaterial to promote new bone formation and is utilized clinically for bone repair and regeneration. We investigated gene expression patterns of osteogenic differentiation in human periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA arra.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1329
- DOI:
- 10.4238/vol10-3gmr1329
Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells
Authors: H. Zhang, F. Chen, G.L. Li, Y.Y. Ding, Z.R. Tao, J.J. Li,S.L. Zhong and L.Z. Lu
Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.20
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.20
Circulatory microRNA-145 expression is increased in cerebral ischemia
Authors: C.S. Gan, C.W. Wang and K.S. Tan
Cerebral ischemia or ischemic stroke is mainly attributed to vascular and circulation disorders. Among protein biomarkers, RNA profiles have also been identified as markers of ischemic stroke. MicroRNA-145 expression is ostensibly recognized as marker and modulator of vascular smooth muscle cell phenotype; however, expression .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.27.1
- DOI:
- http://dx.doi.org/10.4238/2012.January.27.1
HIV TAT variants differentially influence the production of glucocerebrosidase in Sf9 cells
Authors: Andrea K. Vaags, Tessa N. Campbell and Francis Y.M. Choy
Gaucher disease, the most common lysosomal storage disorder, is currently treated with enzyme replacement therapy. This approach, however, is ineffective in altering the progression of neurodegeneration in type 2 and type 3 patients due to the difficulty of transferring the recombinant enzyme across the blood-brain barrier. Hu.. Read More»
- Genet. Mol. Res. 4(3):
Support vector machines for novel class detection in Bioinformatics
Authors: Eduardo J. Spinosa and Andr�© C.P.L.F. de Carvalho
Novelty detection techniques might be a promising way of dealing with high-dimensional classification problems in Bioinformatics. We present preliminary results of the use of a one-class support vector machine approach to detect novel classes in two Bioinformatics databases. The results are compatible with theory and inspire f.. Read More»
- Genet. Mol. Res. 4(3):
Gene selection based on multi-class support vector machines and genetic algorithms
Authors: Bruno Feres de Souza and Andr�© Ponce de Leon F. de Carvalho
Microarrays are a new technology that allows biologists to better understand the interactions between diverse pathologic state at the gene level. However, the amount of data generated by these tools becomes problematic, even though data are supposed to be automatically analyzed (e.g., for diagnostic purposes). The issue become.. Read More»
- Genet. Mol. Res. 4(3):
Position effect variegation and epigenetic modification of a transgene in a pig model
Authors: Z. Yin, Q.R. Kong, Z.P. Zhao, M.L. Wu, Y.S. Mu, K. Hu1 and Z.H. Liu
Sequences proximal to transgene integration sites are able to regulate transgene expression, resulting in complex position effect variegation. Position effect variegation can cause differences in epigenetic modifications, such as DNA methylation and histone acetylation. However, it is not known which factor, position effect or.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.16.1
- DOI:
- 10.4238/2012.February.16.1
Differential gene expression and functional analysis of pit cells from regenerating rat liver
Authors: C.S. Xu, X.G. Chen, C.F. Chang, G.P. Wang, W.B. Wang, L.X. Zhang, Q.S. Zhu and L. Wang
Hepatic pit cells are a population of large granular lymphocytes that substantially contribute to hepatic immunity. Studies have proven that pit cells have a role in liver regeneration, but the details of the relationship between pit cells and liver regeneration is not clear at present. We subjected rats to a two-third hepatectomy; pit cells with high pur.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1052
- DOI:
- 10.4238/vol10-2gmr1052
Molecular cloning, characterization and expression analysis of a Doublesex gene from Daphnia carinata (Crustacea: Cladocera) during different reproductive stages
Authors: M.Q. Zhang, C.A. Ma, W.W. Lv, Y.H. Huang, D.L. Wang and Y.L. Zhao
To better understand the reproductive transformation mechanism of Daphnia carinata, a Doublesex (Dsx) gene was cloned based on rapid amplification of cDNA ends (RACE), and was designated DapcaDsx2. Next, we compared similarities and assumed homology based on deduced amino acid sequences. It showed 97.52, 87.94, and 85.11% identity to orthologous genes in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.10
- DOI:
- 10.4238/2015.June.1.10