Expression
Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs
Authors: Y. Gao, Y.H. Zhang, H. Jiang, S.Q. Xiao, S. Wang, Q. Ma, G.J. Sun, F.J. Li, Q. Deng, L.S. Dai, Z.H. Zhao, X.S. Cui, S.M. Zhang, D.F. Liu and J.B. Zhang
Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese c.. Read More»
- Genet. Mol. Res. 10(2):
Parathyroid hormone-related peptide (PTHrP): prokaryotic expression, purification, and preparation of a polyclonal antibody
Authors: H.L. Zheng, H. Li, Y.S. Sun, Z.Y. Yang and Q. Yu
Parathyroid hormone-related peptide (PTHrP) plays important roles in promoting cancer occurrence and in the development of bone metastases. To increase our knowledge of the biological functions of PTHrP, the prokaryotic expression vector pET-PTHrP was successfully constructed and the His-PTHrP fusion protein was expressed in .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.25.8
- DOI:
- 10.4238/2014.August.25.8
Cloning and expression analysis of the Lonicera japonica Thunb. chlorogenic acid synthetase gene (LjCCoAOMT1) in rice
Authors: X.-H Jiang , C.-W She, Y.-H Zhu and X.-M Liu
Complete coding DNA sequences of a closely related chlorogenic acid synthetase gene (LjCCoAOMT1) were isolated from Lonicera japonica Thunb. by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). LjCCoAOMT1 was subsequently overexpressed in Escherichia coli and a 25-kD protein .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.26.5
- DOI:
- 10.4238/2014.March.26.5
Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.)
Authors: L.-L. Guan, W. Wu, B. Hu, D. Li, J.-W. Chen, K. Hou and L. Wang
Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.7
- DOI:
- 10.4238/2014.August.28.7
Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide
Authors: L. Fu, Y.L. Hou, X. Ding, Y.J. Du, H.Q. Zhu, N. Zhang and W.R. Hou
The complementary DNA (cDNA) of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide (FTL) gene was successfully cloned using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing FTL cDNA and overexpressed it in Escherichia coli using pET28a plasmi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038593
- DOI:
- 10.4238/gmr.15038593
Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines
Authors: J. Ogorevc and P. Dov?
Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.12
Effects of the antipsoriatic drug dithranol on E2A and caspase-9 gene expression in vitro
Authors: C. Ronpirin and T. Tencomnao
Although the precise causes of psoriasis remain to be elucidated, psoriasis has been known as a disorder in which factors in the immune system, enzymes and other biochemical substances that regulate skin cell division are functionally imbalanced, thereby resulting in rapid proliferation of keratinocytes and incomplete keratini.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.17.3
- DOI:
- 10.4238/2012.February.17.3
Expression and antibody generation of the cancer-testis antigen, BIOT2-S
Authors: J.-Y. Wang, M. Cao, M.-R. Guo, S. Li, X.-F. Yang, M. Wang, J. Fang and J. Zhao
Biot2-S is a mouse cancer-testis antigen gene that was identified using the cross-reactive serological analysis of recombinant cDNA expression libraries (SEREX) technique in the State Key Laboratory of Biotherapy, West China Hospital, Sichuan University. To express BIOT2-S and generate its antibody for further investigation, the Biot2-S prokaryotic recomb.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.17
- DOI:
- 10.4238/2015.July.31.17
mRNA expression of prolactin receptor in sheep pituitary and its effect on maternal behavior
Authors: L.P. Wang
Prolactin receptor (PRLR) is the specific receptor for prolactin. Brain-specific control of PRLR gene expression is correlated with the induction of maternal behavior. The aim of this study was to investigate the dynamic changes in the PRLR mRNA level in the sheep pituitary gland and its effect on ewe maternal behavior. The mRNA expression of PRLR during .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.13
- DOI:
- 10.4238/2015.July.31.13
Identification and characterization of the 14-3-3 gene family in switchgrass
Authors: S. Wu, H.D. Yan, A.L. Zhang, L.K. Huang, G.H. Yin and S. Lee
Members of the 14-3-3 family of proteins are conserved regulatory proteins that are widely found in eukaryotes and play crucial roles in diverse physiological processes, including responses to different stresses. Although genome-wide analysis of 14-3-3 proteins has been performed in a few plant species, it has not been perform.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048688
- DOI:
- 10.4238/gmr15048688