Expression
Study of Hgp44 from Porphyromonas gingivalis on inducing HUVECs to secrete IL-6 and IL-8
Authors: J. Zhang, H.H. Cui1, Y. Wang, Q.X. Zhang, S.L. Deng, Y.D. Chen and H. Chen
The aim of this study was to clone, express the gene of Hgp44 in adhesin domains of gingipains from Porphyromonas gingivalis and purify the protein. Furthermore, the effect of Hgp44 from P. gingivalis on inducing HUVECs to secrete IL-6 and IL-8 was evaluated. The Hgp44 gene fragment was amplified by polymerase chain reaction, .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.1
- DOI:
- 10.4238/2014.March.31.1
Molecular characterization and expression pattern of an �±-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii
Authors: Molecular characterization and expression pattern of an �±-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii
The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.10
- DOI:
- 10.4238/2014.August.28.10
Isolation and partial characterization of a root-specific promoter for stacking multiple traits into cassava (Manihot esculenta CRANTZ)
Authors: M.A. Gbadegesin and J.R. Beeching
Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr892
- DOI:
- 10.4238/vol10-2gmr892
Acidic and basic fibroblast growth factor expression levels in cervical cancer and their effects on tumor cell proliferation
Authors: Q.H. Zhang, P. Xu, Y.X. Lu and H.T. Dou
Fibroblast growth factors (FGFs) play important roles in angiogenesis, wound healing, embryonic development, and endocrine signaling pathways. Increasingly, recent studies have reported aberrant FGF expression in various malignancies. However, the involvement of FGFs in cervical carcinoma pathogenesis remains unclear. We aimed.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049043
- DOI:
- 10.4238/gmr15049043
Gene expression in the lignin biosynthesis pathway during soybean seed development
Authors: A. Baldoni, E.V.R. Von Pinho, J.S. Fernandes, V.M. Abreu and M.L.M. Carvalho
The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are sca.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.February.28.2
- DOI:
- 10.4238/2013.February.28.2
Identification and characterization of SREBF2 expression and its association with chicken carcass traits
Authors: F. Ye, M.H. Qiu, H.Y. Xu, X. Lan, Q. Zhu, X.L. Zhao, H.D. Yin, Y.P. Liu and Y. Wang
The sterol regulatory element-binding transcription factor 2 gene (SREBF2) plays an important role in regulating lipid homeostasis. To reveal the genetic factors that underlie carcass fat deposition in chickens, we cloned the coding DNA sequence of chicken SREBF2, investigated SREBF2 mRNA expression levels in various tissues, .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038514
- DOI:
- 10.4238/gmr.15038514
Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li*, J.T. Lin, H.G. Feng and Z.Q. Yuan
We used a simple and efficient method to construct a bicistronic eukaryotic expression vector pIRES2-LIF-NT-3. The leukemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The LIF cDNA fragment was inserted into.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.June.18.12
- DOI:
- 10.4238/2014.June.18.12
Construction and identification of pIRES2-VEGF165-NT-3 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li, H.G. Feng, J.T. Lin and Z.Q. Yuan
We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-VEGF165-NT-3. The neurotrophin-3 (NT-3) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NT-3 cDNA fragment was cloned into the pIRES2-VEGF165-EGFP vector in.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.June.18.13
- DOI:
- 10.4238/2014.June.18.13
Regulation of WNK4 gene transcription in the kidneys
Authors: Z.J. Sun, Y. Li, Y.H. Li, J.S. Zhang, Q. Ding, J. Li-Ling, Y. Liang and Y.Y. Zhao
With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoaldosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mech.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.9
- DOI:
- 10.4238/2013.January.4.9
Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions
Authors: I. Aquino, M.S.F. Tsuboy, J.C. Marcarini, M.S. Mantovani, F.F. Perazzo and E.L. Maistro
The malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to eval.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.24.6
- DOI:
- 10.4238/2013.July.24.6