Expression
Cloning of three genes involved in the flavonoid metabolic pathway and their expression during insect resistance in Pinus massoniana Lamb.
Authors: Z.Q. Yang, H. Chen, J.H. Tan, H.L. Xu, J. Jia and Y.H. Feng
Pinus massoniana Lamb. is an important timber and turpentine-producing tree species in China. Dendrolimus punctatus and Dasychira axutha are leaf-eating pests that have harmful effects on P. massoniana production. Few studies have focused on the molecular mechanisms underlying pest resistance in P. massoniana. Based on sequenc.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049332
- DOI:
- 10.4238/gmr15049332
Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.)
Authors: J. Zhang, X.L. Sun, L.G. Zhang, M.X. Hui and M.K. Zhang
Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.24.5
- DOI:
- 10.4238/2013.July.24.5
ABCB1 gene expression in peripheral blood mononuclear cells in healthy Thai males and females
Authors: P. Sudchada, S. Oo-puthinan, O. Kerdpin and N. Saelim
We compared the expression of the ABCB1 gene in healthy male and female Thai subjects; this gene encodes the P-glycoprotein transporter in peripheral blood mononuclear cells (PBMCs). We also identified the most suitable housekeeping genes for normalization of ABCB1 expression levels in PBMCs. PBMCs from 30 females and 26 males.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr813
- DOI:
- 10.4238/vol9-2gmr813
DKK1 eukaryotic expression plasmid and expression product identification
Authors: G.Y. Bao, K.Y. Lu, S.F. Cui and L. Xu
We constructed the human dickkopf 1 (DKK1) eukaryotic expression plasmid and expressed, purified, and identified its expression product. We extracted cancer cells from cervical cancer tissue, followed by extraction of mRNA. Reverse transcription-polymerase chain reaction was conducted to obtain DKK1 gene fragments. Using these fragments, we prepared the r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.5
- DOI:
- 10.4238/2015.June.11.5
Molecular characterization of the gene checkpoint homolog 1 in Daphnia carinata during different reproductive phases
Authors: L. Kong, H.X. Li, D.L. Wu, G.R. Xu, D.L. Wang and Y.L. Zhao
Full-length cDNA of the gene checkpoint homolog 1 (Chk1) was cloned from Daphnia carinata and designated DcarChk1. DcarChk1 cDNA was 1817 bp in length and encoded a 497-amino acid polypeptide. Phylogenetic analyses revealed that DcarChk1 was most closely related to Chk1 of Daphnia pulex, followed by homologous genes of insects.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026132
- DOI:
- 10.4238/gmr.15026132
Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression
Authors: W. Gao, W. Sun, R. Su, X.Y. Lv, Q.Z. Wang, D. Li, H.H. Musa, L. Chen, H. Zhou, H.S. Xu and W.H. Hua
RT-PCR was used to study the temporal and spatial pattern of Yes-associated protein 1 (YAP1) and myosin heavy chain (MyHC) expression in four different skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and three growth stages (i.e., 2 days old, 2 and 6 months .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027260
- DOI:
- 10.4238/gmr.15027260
Molecular characterization and expression analysis of the �²-actin gene from the ridgetail white prawn Exopalaemon carinicauda
Authors: J.P. Liang, Y. Wang, Q.Q. Ge, J.T. Li, P. Liu, J. Li and G.X. Nie
Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026872
- DOI:
- 10.4238/gmr.15026872
Relationship between TLR4 and MCP2 expression levels and habitual abortion
Authors: X.P. Li, L.N. Song, L.P. Tian and Y.S. Zhang
Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027378
- DOI:
- 10.4238/gmr.15027378
Chicken skeletal muscle-associated macroarray for gene discovery
Authors: E.C. Jorge, C.M.R. Melo, M.F. Ros�¡rio, J.R.S. Rossi, M.C. Ledur, A.S.A.M.T. Moura and L.L. Coutinho
Macro- and microarrays are well-established technologies to determine gene functions through repeated measurements of transcript abundance. We constructed a chicken skeletal muscle-associated array based on a muscle-specific EST database, which was used to generate a tissue expression dataset of ~4500 chicken genes across 5 ad.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr712
- DOI:
- 10.4238/vol9-1gmr712
Effect of cyclosporin A particles of varying diameters on gastric cancer cell apoptosis
Authors: X.L. Xing, Y. Lu and H.L. Qiu
Human health is significantly threatened by gastric cancer, which is the most common malignant tumor; although drastic, surgery is currently the only way to cure it. However, high recurrence rates and low survival rates are associated with the disease. Therefore, to improve the effectiveness of gastric cancer treatment and to .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028085
- DOI:
- 10.4238/gmr.15028085