Expression

Agrobacterium-mediated transformation of the �²-subunit gene in 7S globulin protein in soybean using RNAi technology

Authors: J. Qu, S.Y. Liu, P.W. Wang, S.Y. Guan, Y.G. Fan, D. Yao, L. Zhang and J.L. Dai

The objective of this study was to use RNA interference (RNAi) to improve protein quality and decrease anti-nutritional effects in soybean. Agrobacterium tumefaciens-mediated transformation was conducted using RNAi and an expression vector containing the 7S globulin β-subunit gene. The BAR gene was used as the selective m.. Read More»

Genet. Mol. Res. 15(2):

gmr.15027561

DOI:

10.4238/gmr.15027561

Methylation differences and expression profiles of the caprine DIO3 gene

Authors: H. Zhang, P.F. Jin, L.L. Niu, L. Li, L.J. Wang, Y. Chen, G.J. Zhang, H.P. Zhang and T. Zhong

DIO3 gene encoding type 3 iodothyronine deiodinase is an imprinted gene, located in the DLK1-DIO3 (delta-like 1 homolog-type 3 iodothyronine deiodinase) imprinted domain, and is potentially involved in degrading excessive amounts of thyroid hormone to protect embryogenesis. However, the underlying regulatory mechanism of the imprinted DIO3 gene expression.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038678

DOI:

10.4238/gmr.15038678

Microarray analysis of differentially expressed genes in the liver between Bai�er layers and broilers

Authors: Q.-G. Wang , H.-F. Zhang, S.-Z. Wang, G.L. Gao, L. Leng, W. Na and H. Li

In this study, we profiled gene expression in chicken liver and screened differentially expressed genes in the Bai’er layers and fat line broilers. Birds were derived from the 14th generation of Northeast Agricultural University fat broiler lines and Bai’er layers. Chicken genome arrays were used to screen differentially expressed genes in liv.. Read More»

Genet. Mol. Res. 14(1):

2015.March.31.19

DOI:

10.4238/2015.March.31.19

Biochemical and molecular studies of copper metabolism during the infection process of the human pathogenic fungus Paracoccidioides brasiliensis

Authors: R.S. Santos

Genet. Mol. Res. 9(1):

vol9-1TA028

DOI:

10.4238/vol9-1TA028

Detection and expression of bovine papillomavirus in blood of healthy and papillomatosis-affected cattle

Authors: M.A.R. Silva, B.M.F. De Albuquerque, N.E. Pontes, L.C.A. Coutinho, M.C.G. Leit�£o, M.C. Reis, R.S. Castro and A.C. Freitas

Papillomaviruses (PV) are double-stranded DNA viruses that can cause benignant and malignant tumors in amniotes. There are 13 types of bovine papillomavirus (BPV-1 to -13); they have been found in reproductive tissues and body fluids. Normally these viruses are detected in epithelial tissue. We looked for BPV in the blood of healthy cattle and cattle with.. Read More»

Genet. Mol. Res. 12(3):

2013.February.28.31

DOI:

10.4238/2013.February.28.31

Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits

Authors: T.S. Xu, L.H. Gu, X.H. Zhang, B.G. Ye, X.L. Liu and S.S. Hou

Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation. To identify the characteristics of the Pekin duck MSTN gene and the relationship between its polymorphism and breast muscle traits in Pekin duck, cDNA cloning an.. Read More»

Genet. Mol. Res. 12(3):

2013.February.28.18

DOI:

10.4238/2013.February.28.18

Molecular cloning and expression analyses of RPS3a gene from mulberry under abiotic stresses and among different mulberry varieties

Authors: J. Qian, H. Zhou, M.D. Zhao, H. Wang, F. Li, Y.H. Wang, R.J. Fang, W.G. Zhao and H.J. Kim

A full-length cDNA sequence coding ribosomal protein S3a of mulberry tree, which we designated MmRPS3a (GenBank accession No. KR610331), was cloned based on mulberry expressed sequence tags. Sequence analysis showed that the MmRPS3a is 1089 bp long and contains a 80-bp 5'-UTR (untranslated region) and a 220-bp 3'-UTR. Its open.. Read More»

Genet. Mol. Res. 15(2):

gmr.15028129

DOI:

10.4238/gmr.15028129

Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep

Authors: Y. Yue, X. Cheng, S.G. Zhao, Z. Liu, L.S. Liu, R. Zhou, J.P. Wu and M.A. Brown

To evaluate stearoyl-CoA desaturase (SCD), hormone-sensitive lipase (HSL), lipoprotein lipase (LPL), and peroxisome proliferator-activated receptor (PPARγ) expression in Lanzhou fat-tailed sheep (with and without docked tails), 18 rams were randomly divided into two equal groups (docked group, LT; control group, LC). The.. Read More»

Genet. Mol. Res. 15(1):

gmr.15017323

DOI:

10.4238/gmr.15017323

Molecular cloning and functional analysis of MRLC2 in Tianfu, Boer, and Chengdu Ma goats

Authors: H.G. Xu, G.Y. Xu, L. Wan and J. Ma

To determine the molecular basis of heterosis in goats, fluorescence quantitative polymerase chain reaction (PCR) was performed to investigate myosin-regulatory light chain 2 (MRLC2) gene expression in the longissimus dorsi muscle tissues of the Tianfu goat and its parents, the Boer and Chengdu Ma goats. The goat MRLC2 gene was differentially expressed in.. Read More»

Genet. Mol. Res. 12(3):

2013.March.15.7

DOI:

10.4238/2013.March.15.7

Roles of functional NFKB1 and �²-TrCP insertion/deletion polymorphisms in mRNA expression and epithelial ovarian cancer susceptibility

Authors: Z.H. Huo, H.J. Zhong, Y.S. Zhu, B. Xing and H. Tang

Epithelial ovarian cancer (EOC) is the leading cause of death among all gynecological cancers. Nuclear factor-kappa B (NF-κB) is involved in carcinogenesis and in the development of EOC. The β-transducin repeat-containing protein (β-TrCP) is a positive regulator of the NF-κB signaling pathway. Recent studies have indicated that the -.. Read More»

Genet. Mol. Res. 12(3):

2013.March.11.6

DOI:

10.4238/2013.March.11.6