Expression
Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique
Authors: D.F. Barione, F.S.N. Lizarte, P.C. Novais, C.A.M. de Carvalho, F.C.B. Valeri, F.M. Peria, H.F. de Oliveira, D.L. Zanette, W.A. Silva Jr., A.J. Cologna, R.B. Reis, S. Tucci Jr., A.C.P. Martins, D.P.C. Tirapelli and L.F. Tirapelli
We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples f.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.4
- DOI:
- 10.4238/2013.April.2.4
Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line
Authors: S.Y. Wang, J.L. Huo, Y.W. Miao, W.M. Cheng and Y.Z. Zeng
U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.9
- DOI:
- 10.4238/2013.April.2.9
cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)
Authors: Q. Zou, P. Wei, Q. Xu, H.Z. Zheng, B. Tang and S.G. Wang
The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and funct.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.7
- DOI:
- 10.4238/2013.April.2.7
Protein expression levels in the medullary visceral zone of rats with subarachnoid hemorrhage
Authors: L.H. Sun, L.F. Xing, G.H. Zhang and S.Y. Pan
We investigated protein expression in the medullary visceral zone (MVZ) of rats with multiple-organ dysfunction syndrome (MODS) caused by subarachnoid hemorrhage (SAH) to discuss the possible regulatory mechanism of the MVZ in the course of SAH-induced MODS. A SAH-induced MODS model was established in rats by injecting arterial blood into the Willis&rsquo.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.3.7
- DOI:
- 10.4238/2015.August.3.7
Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells
Authors: X.Y. Wang, J.H. Zhang, Q.L. Sun, Z.Y. Yao, B.G. Deng, W.Y. Guo,L. Wang, W.H. Dong, F. Wang, C.P. Zhao and T.Y. Wang
Preliminary studies have suggested that a characteristic element of the matrix attachment region (MAR) in human interferon-β mediates the adhesion of vectors to Chinese hamster ovary (CHO) cells. In this study, we investigated if vector adhesion increased nerve growth factor (NGF) expression in CHO cells. The MAR characteristic element sequence of hu.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.29
- DOI:
- 10.4238/2015.August.7.29
Effects of conjugated linoleic acid on the expression levels of miR-27 and miR-143 inpig adipose tissue
Authors: R.L. Qi, Y. Chen, J.X. Huang andF.Y. Yang
In this study, we evaluated the effect and possible mechanism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were collected, and real-time polymer.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.7
- DOI:
- 10.4238/2015.June.26.7
Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides
Authors: Y.B. Bao, L. Li, M.X. Ye, Y.H. Dong, W.X. Jin and Z.H. Lin
The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa (Tg-GS) from hemocytes by 3'- and 5'-rapid amplification .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.9
- DOI:
- 10.4238/2013.April.10.9
S-adenosylmethionine, a methyl donor, up regulates tissue inhibitor of metalloproteinase-2 in colorectal cancer
Authors: Z. Hussain, M.I. Khan, M. Shahid and F.N. Almajhdi
DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloprotein.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.6
- DOI:
- 10.4238/2013.April.10.6
Expression and diagnostic value of proteins in Mycobacterium tuberculosis
Authors: Z.Y. Zhu, Y. Liu, H.B. Wang, J.Z. Xiao, Y.F. Qiu, L. Yan, D. Chen, A.G. Liu and X. Yang
We constructed a prokaryotic expression vector expressing the Mycobacterium tuberculosis protein TB16.3, as well as 3 other proteins, including TB15.3, CFP-10, and Rv2626C, which were purified and analyzed for their effectiveness as detection antibodies. The TB16.3 genes of M. tuberculosis H37Rv geno.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.16
- DOI:
- 10.4238/2014.September.26.16
Modification of vectors for functional genomic analysis in plants
Authors: J.T. Li, G. Yu, X.H. Sun, C.G. Jia, Q. Du, Q.Y. Li and H.Y. Pan
Simple, efficient, and economical recombinant plant binary expression vectors for deciphering large-scale functional genomic research in plants and promoting crop improvement by genetically engineering and biotechnology is in great demand. In this research, using the pCHF3, pCAMBIA1301, pCAMBIA3300, pCAMBIA3301 vectors, we suc.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.20
- DOI:
- 10.4238/2014.September.26.20