Expression
Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)
Authors: R.A. Khan1, M. Rashid1, D. Wang2 and Y.L. Zhang1
Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression ve.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.3
- DOI:
- 10.4238/2013.October.10.3
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
Authors: I.B. Cordeiro1, D.P. Castro7, P.P.O. Nogueira7, P.C.S. Angelo6, P.A. Nogueira7, J.F.C. Gon�§alves8, A.M.R.F. Pereira9, J.S. Garcia2,3, G.H.M.F. Souza2,4, M.A.Z. Arruda5, M.N. Eberlin2, S. Astolfi-Filho1, E.V. Andrade1 and J.L. L�³pez-Lozano10
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.1
- DOI:
- 10.4238/2013.October.29.1
A new reliable reference gene UBA52 for quantitative real-time polymerase chain reaction studies in pyloric cecal tissues of the starfish Asterias rubens
Authors: A.F. Sadritdinova, A.A. Dmitriev, A.V. Snezhkina, M.S. Belenikin, G.S. Krasnov, O.G. Manylov, A.A. Kudryavtsev, N.V. Melnikova, A.S. Speranskaya, M.V. Darii, V.A. Lakunina, L.A. Uroshlev, A.O. Smurov, O.A. Stepanov and A.V. Kudryavtseva
The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas.This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.8
Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor
Authors: Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor
The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage to its native host Apis cerana. Understanding the resistance mechanisms of eastern bees against Varroa mites will help researchers determine how to protect other species from this organism. The A. cerana genome has not been previo.. Read More»
- Genet. Mol. Res. 13(4):
Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)
Authors: Y.L. Hou, X. Ding, W. Hou, B. Song, T. Wang, F. Wang, J. Li, J. Zhong, T. Xu, B.X. Ma, H.Q. Zhu, J.H. Li and J.C. Zhong
The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain rea.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.11
- DOI:
- 10.4238/2013.October.18.11
Expression of cationic amino acid transporters, carcass traits, and performance of growing pigs fed low-protein amino acid-supplemented versus high protein diets
Authors: A. Morales, F. Grageola, H. GarcÃ?Âa, A. Araiza, R.T. Zijlstra and M. Cervantes
Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.9
- DOI:
- 10.4238/2013.October.18.9
Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense
Authors: Y.P. Zhang, H. Qiao, W.Y. Zhang, S.M. Sun, S.F. Jiang, Y.S. Gong, Y.W. Xiong, S.B. Jin and H.T. Fu
In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-ti.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.8
- DOI:
- 10.4238/2013.October.18.8
Molecular cloning, sequence characterization, and gene expression profiling of a novel water buffalo (Bubalus bubalis) gene, AGPAT6
Authors: S. Song, J.L. Huo,D.L. Li, Y.Y. Yuan, F. Yuan and Y.W. Miao
Several 1-acylglycerol-3-phosphate-O-acyltransferases (AGPATs) can acylate lysophosphatidic acid to produce phosphatidic acid. Of the eight AGPAT isoforms, AGPAT6 is a crucial enzyme for glycerolipids and triacylglycerol biosynthesis in some mammalian tissues. We amplified and identified the complete coding sequence (CDS) of the water buffalo AGPAT6 gene .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.1.2
- DOI:
- 10.4238/2013.October.1.2
Expression profile of insulin-like growth factor system genes in muscle tissues during the postnatal development growth stage in ducks
Authors: C.-L. Song, H.-H. Liu, J. Kou, L. Lv, L. Li, W.-X. Wang and J.-W. Wang
Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (pos.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.May.6.3
- DOI:
- 10.4238/2013.May.6.3
Aberrant gene expression profiles in hepatocellular carcinoma detected by microdissection
Authors: Y.M. Wei1, Y.Y. Li2, Y.C. Zhang2 and Y.Q. Nie2
The aim of this study was to identify genomic aberrations in hepatocellular carcinoma (HCC) by using laser capture microdissection (LCM) combined with microarray analysis. Samples were procured by LCM from HCC and patient-matched normal liver tissue surgically resected from 4 patients. RNA was isolated from the samples and reverse transcribed into cDNA. A.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.3
- DOI:
- 10.4238/2013.November.18.3