Expression
Genome-wide identification and phylogenetic analysis of the AP2/ERF gene superfamily in sweet orange (Citrus sinensis)
Authors: T.M. Ito, P.B. Polido, M.C. Rampim, G. Kaschuk and S.G.H. Souza
Sweet orange (Citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. The stress tolerance could be addressed by manipulating the ethylene response factor (ERF) transcription factors because they orchestrate .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.22
- DOI:
- 10.4238/2014.September.26.22
Construction of overexpression vectors of Magnaporthe oryzae genes BAS1 and BAS4 fusion to mCherry and screening of overexpression strains
Authors: M.L. Liang, J.L. Yan, Y.Q. Yang, L. Liu, C.Y. Li and J. Yang
The aim of this study was to construct overexpression vectors and selecting strains of the Magnaporthe oryzae effectors BAS1 and BAS4. Primer pairs of BAS1, BAS4, and mCherry were designed based on their known nucleotide sequences. The coding sequences of BAS1 and BAS4 were amplified, and the pXY201 plasmid was selected as a template to amplify the mCherr.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.17
- DOI:
- 10.4238/2015.June.26.17
Identification and expression analysis of YABBY family genes associated with fruit shape in tomato (Solanum lycopersicum L.)
Authors: H.Q. Han, Y. Liu, M.M. Jiang, H.Y. Ge and H.Y. Chen
YABBY family genes play important roles in the development of leaf, flower, and fruit. The purpose of this research was to integrate all the YABBY genes and analyze the correlation between gene expression and fruit shape in tomato. Scanning of 24 genomes of sequenced species demonstrated that YABBY genes were very normal and stable in flowering plants exc.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.1
- DOI:
- 10.4238/2015.June.29.1
Molecular cloning and tissue expression analyses of two novel pepper genes: heterotrimeric G protein beta 2 subunit and ArcA1
Authors: M.H. Deng, J.L. Huo, H.S. Zhu, H. Zhou and J.F. Wen
We isolated two transcription factor genes, heterotrimeric G protein beta 2 subunit (Gβ2) and ArcA1, from pepper (Capsicum annuum). The complete coding sequences were amplified using reversed transcriptase PCR based on conserved sequence information of Solanum lycopersicum and several other plant species. Nucleotide sequence analysis of these two gen.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.1
- DOI:
- 10.4238/2013.April.17.1
Construction of suppressor of cytokine signaling 2 (SOCS2) adenoviral overexpression vector and its impact on growth hormone- induced lipolysis in swine primary adipocytes
Authors: H.L. Yang, J. Yan, M. Feng, X. Tan, G.Y. Yan, L. Gan and C. Sun
We investigated the effect of overexpression suppressor of cytokine signaling 2 (SOCS2) on lipolysis in swine primary adipocytes (pAd) induced by growth hormone (GH). We constructed pAd-SOCS2 adenoviral overexpression vectors to infect HEK293 cells for virus packaging and propagation. Cultured swine primary adipocytes were infected with virus particles; a.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.January.9.2
- DOI:
- 10.4238/2013.January.9.2
Genome-wide analysis of TCP family in tobacco
Authors: L. Chen, Y.Q. Chen, A.M. Ding, H. Chen, F. Xia, W.F. Wang and Y.H. Sun
The TCP family is a transcription factor family, members of which are extensively involved in plant growth and development as well as in signal transduction in the response against many physiological and biochemical stimuli. In the present study, 61 TCP genes were identified in tobacco (Nicotiana tabacum) genome. Bioinformatic.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027728
- DOI:
- 10.4238/gmr.15027728
Screening and analyzing genes associated with Amur tiger placental development
Authors: Q. Li, T.F. Lu, D. Liu, P.F. Hu, B. Sun, J.Z. Ma, W.J. Wang, K.F. Wang, W.X. Zhang, J. Chen, W.J. Guan, Y.H. Ma and M.H. Zhang
The Amur tiger is a unique endangered species in the world, and thus, protection of its genetic resources is extremely important. In this study, an Amur tiger placenta cDNA library was constructed using the SMART cDNA Library Construction kit. A total of 508 colonies were sequenced, in which 205 (76%) genes were annotated and .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.25
- DOI:
- 10.4238/2014.September.26.25
Clinical significance of CTHRC1 protein expression in human cancers: a meta-analysis
Authors: S.F. Wang, Z. Yin, J.J. Yin, W. Zhang and C.G. Dong
The aim of this meta-analysis was to investigate the overall diagnostic and prognostic values of CTHRC1 expression in human cancer development. Based on the inclusion and exclusion criteria, 8 cohort studies were included in the meta-analysis. The data were extracted, and analyses were performed using a random-effects model. S.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027855
- DOI:
- 10.4238/gmr.15027855
Influence of gender on ABCC2 expression in peripheral blood mononuclear cells
Authors: P. Sudchada, W. Chareanchim, A. Assawamakin, P. Thaipiya, W. Choochaimongkhol, N. Thiplui and P. Sukmangsa
It is known that several factors, including gender, may influence the expression of multidrug resistance associated proteins 2 (MRP2/ABCC2) in the peripheral blood mononuclear cells (PBMCs). This study aims to compare ABCC2 gene expression in PBMCs of healthy males and females. PBMCs were extracted from 48 females and 44 males, and gene expression was mea.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.18
- DOI:
- 10.4238/2015.December.11.18
Screening for key genes associated with invasive ductal carcinoma of the breast via microarray data analysis
Authors: J.L. Huan, X. Gao, L. Xing, X.J. Qin, H.X. Qian, Q. Zhou and L. Zhu
The aim of this study was to identify key genes related to invasive ductal carcinoma (IDC) of the breast by analyzing gene expression data with bioinformatic tools. Microarray data set GSE31138 was downloaded from Gene Expression Omnibus, including 3 breast cancer tissue samples and 3 normal controls. Differentially expressed .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.5
- DOI:
- 10.4238/2014.September.29.5