Expression
Dietary glycerol for quail: association between productive performance and COX III mRNA expression
Authors: S.C.C. Silva, E. Gasparino, E. Batista, F. Tanamati, A.P.D. Vesco, B. Lala and D.P. de Oliveira
This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake wer.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027435
- DOI:
- 10.4238/gmr.15027435
Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense
Authors: N.M.A. Rahman, H. Fu, H. Qiao, S. Jin, H. Bai, W. Zhang, F.W. Jiang, G. Liang, S. Sun, Y. Gong, F.F. Jiang, Y. Xiong and Y. Wu
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different deve.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027950
- DOI:
- 10.4238/gmr.15027950
Isolation and characterization of Calcineurin B-like gene (PbCBL1) and its promoter in birch-leaf pear (Pyrus betulifolia Bunge)
Authors: Y.Y. Xu, H. Li, J. Lin, X.G. Li and Y.H. Chang
Calcium plays a critical role in regulating abiotic stress responses in plants. Calcineurin B-like (CBL) proteins are calcium sensors in calcium signaling pathways. However, the molecular mechanisms underlying calcium signaling remain to be elucidated. In this study, the CBL1 gene, which codes for the CBL protein, was isolated from the birch-leaf pear. On.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.3
- DOI:
- 10.4238/2015.December.14.3
miR-218 tissue expression level is associated with aggressive progression of gastric cancer
Authors: X.X. Wang, S.J. Ge, X.L. Wang, L.X. Jiang, M.F. Sheng and J.J. Ma
The aim of the present study was to investigate the clinical significance of microRNA-218 (miR-218) in gastric cancer. We enrolled 112 patients having undergone surgery for gastric cancer between May 2008 and June 2014. Expression of miR-218 was determined by real-time quantitative reverse transcription-polymerase chain reacti.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027521
- DOI:
- 10.4238/gmr.15027521
A structured sparse regression method for estimating isoform expression level from multi-sample RNA-seq data
Authors: L. Zhang and X.J. Liu
With the rapid development of next-generation high-throughput sequencing technology, RNA-seq has become a standard and important technique for transcriptome analysis. For multi-sample RNA-seq data, the existing expression estimation methods usually deal with each single-RNA-seq sample, and ignore that the read distributions ar.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027670
- DOI:
- 10.4238/gmr.15027670
Deep sequencing-based analysis of gene expression in bovine mammary epithelial cells after Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae infection
Authors: L. Xiu, Y.B. Fu, Y. Deng, X.J. Shi, Z.Y. Bian, A. Ruhan and X. Wang
The goal of this study was to characterize the transcriptome of primary bovine mammalian epithelial cells (pBMECs) and to identify candidate genes for response and resistance to Staphylococcus aureus (strain S108), Escherichia coli (strain E23), and Klebsiella pneumoniae (strain K96) infection. Using Solexa sequencing, approximately 4.9 million total sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.1
- DOI:
- 10.4238/2015.December.15.1
Effect of Fimbristylis ovata on receptor for advanced glycation end-products,proinflammatory cytokines, and cell adhesion molecule level and gene expression in U937 and bEnd.3 cell lines
Authors: S. Sukjamnong and R. Santiyanont
Fimbristylis ovata has been long used as a traditional medicine for chronic inflammatory diseases; however, there are no data regarding its anti-inflammatory properties. In this study, we investigated the effects of F. ovata extracts on the secretion of pro-inflammatory cytokines, cell adhesion molecule, and receptor for advanced glycation end-products (R.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.13
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.13
Overexpression of pucC improves the heterologous protein expression level in a Rhodobacter sphaeroides expression system
Authors: L. Cheng,G. Chen,G. Ding,Z. Zhao,T. Dong and Z. Hu
The Rhodobacter sphaeroides system has been used to express membrane proteins. However, its low yield has substantially limited its application. In order to promote the protein expression capability of this system, the pucC gene, which plays a crucial role in assembling the R. sphaeroides light-harvesting 2 complex (LH2), was overexpressed. To build a puc.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.21
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.21
Dysregulation of TFDP1 and of the cell cycle pathway in high-grade glioblastoma multiforme: a bioinformatic analysis
Authors: X. Lu, X.D. Lv, Y.H. Ren, W.D. Yang, Z.B. Li, L. Zhang and X.F. Bai
Despite extensive research, the prognosis of high-grade glioblastoma multiforme (GBM) has improved only slightly because of the limited response to standard treatments. Recent advances (discoveries of molecular biomarkers) provide new opportunities for the treatment of GBM. The aim of the present study was to identify diagnost.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027646
- DOI:
- 10.4238/gmr.15027646
Prognostic value of miR-141 downregulation in gastric cancer
Authors: Y.B. Lu, J.J. Hu, W.J. Sun, X.H. Duan and X. Chen
Previous research has shown that microRNA-141 (miR-141) expression levels are associated with survival in several types of cancer. In the present study, we investigated the clinical significance and prognostic value of miR-141 in gastric cancer. Paired tissue specimens (tumor and adjacent normal mucosa) from 95 patients with gastric cancer were obtained a.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.31
- DOI:
- 10.4238/2015.December.16.31