Expression
cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)
Authors: Y. Song, Y.-L. Hou, W.-R. Hou, G.-F. Wu and T. Zhang
The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherich.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1000
- DOI:
- 10.4238/vol10-1gmr1000
Akirin2 expression in response to vaccine-induced immunity in chicken
Authors: C.L. Man, W.T. Mu, Y. Chang and D.X. Zhao
Akirin2 is a nuclear factor that plays an important role in the development and regulation of innate immune response. In this study, akirin2 gene expression in several primary immune organs (liver, thymus, and bursa) of Hi-Line Brown chicken administered with the LoSota vaccine was analyzed during the various stages of increase in Newcastle disease virus .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.20
- DOI:
- 10.4238/2015.December.21.20
C/EBP�± gene as a genetic marker for beef quality improvement
Authors: C. Adoligbe, Y.F. Huangfu, L.S. Zan and H. Wang
Intramuscular fat (IMF) or intramuscular triglycerides are interspersed throughout the skeletal muscles. The IMF, also called marbling, imparts meat with flavor and juiciness and is one of the core criteria for judging carcass value. The quantity of IMF is influenced entirely by genetics. Recently, understanding the underlying genetic bases of IMF has bee.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.1
- DOI:
- 10.4238/2015.August.14.1
Cancer classification based on gene expression using neural networks
Authors: H.P. Hu, Z.J. Niu, Y.P. Bai and X.H. Tan
Based on gene expression, we have classified 53 colon cancer patients with UICC II into two groups: relapse and no relapse. Samples were taken from each patient, and gene information was extracted. Of the 53 samples examined, 500 genes were considered proper through analyses by S-Kohonen, BP, and SVM neural networks. Classification accuracy obtained by S-.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.33
- DOI:
- 10.4238/2015.December.21.33
Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle
Authors: Z.D. Zhao, A.N. Li, S.J. Wei, M.M. Wang, S.J. Li and L.S. Zan
Fatty acid transport protein 1 (FATP1), an integral membrane protein that facilitates long-chain fatty acid influx, is involved in the genetic network for oleic acid synthesis. The aim of this study was to examine the association of FATP1 polymorphisms with live animal meat quality traits in Chinese Qinchuan cattle. Quantitative real-time PCR analysis dem.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.14
- DOI:
- 10.4238/2015.December.21.14
Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids
Authors: M. Cervantes, N. Arce, H. GarcÃ?Âa, M. Cota, J.K. Htoo and A. Morales
Excess Leu reduces the availability of Ile and Val in pigs likely by reducing absorption of the latter amino acids (AA). Twenty-four crossbred pigs were used to evaluate the effect of excess Leu alone or with surplus Ile and Val on the expression of cationic (b0,+AT and CAT1) and neutral (B0AT1) AA transporters in the small intestine, liver, and skeletal .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.11
- DOI:
- 10.4238/2015.August.19.11
Selection of reference genes in canine uterine tissues
Authors: M. Du, X. Wang, Y.W. Yue, P.Y. Zhou, W. Yao, X. Li, X.B. Ding,X.F. Liu, H. Guo and W.Z. Ma
Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relati.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028138
- DOI:
- 10.4238/gmr.15028138
Bayesian forecasting of temporal gene expression by using an auto regressive panel data approach
Authors: M. Nascimento, F.F. e Silva, T. S�¡fadi, A.C.C. Nascimento, L.M.A. Barroso, L.S. Gl�³ria and B. de S. Carvalho
We propose and evaluate a novel approach for forecasting gene expression over non-observed times in longitudinal trials under a Bayesian viewpoint. One of the aims is to cluster genes that share similar expression patterns over time and then use this similarity to predict relative expression at time points of interest. Express.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027299
- DOI:
- 10.4238/gmr.15027299
Expression of recombinant human anti-TNF-�± scFv-Fc in Arabidopsis thaliana seeds
Authors: N. Yao, L. Ai, Y.Y. Dong, X.M. Liu, D.Z. Wang, N. Wang, X.W. Li,F.W. Wang, XK. Li, H.Y. Li and C. Jiang
Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest si.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027726
- DOI:
- 10.4238/gmr.15027726
Differential gene expression patterns in the autogamous plant Hordeum euclaston (Poaceae)
Authors: J.E. Georg-Kraemer, C.A.S. Ferreira and S.S. Cavalli
Sib-seedlings of 95 strains of the strictly autogamous grass Hordeum euclaston were analyzed by horizontal polyacrylamide gel electrophoresis for four isoenzyme systems at a specific ontogenetic stage. We found differences in the activity of some genes among individuals of this species. Hence, an ontogenetic analysis was carri.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1017
- DOI:
- 10.4238/vol10-1gmr1017