Expression
Large-scale tissue-specific and temporal gene expression profiles in Pengze crucian carp
Authors: Y. Zheng, J.Z. Chen, H.P. Wang, M. Li, H.W. Liang, X.W. Bing and Z.Z. Wang
In the present study, the tissue-specific and temporal gene expression profiles of four catalogues of gonadal development-related genes (sex differentiation-related, steroid receptor, steroidogenic, and structural genes) were detected in nine tissues and during 11 successive developmental stages in the Pengze crucian carp (Pcc.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017642
- DOI:
- 10.4238/gmr.15017642
Characterization of porcine GAS6 cDNA gene and its expression analysis in weaned piglets
Authors: D. Xu, J. Jiang, J. He, C.Q. He and H.M. Ma
The growth arrest-specific 6 gene (GAS6) is a member of the family of plasma vitamin K-dependent proteins, which are able to bind to phospholipids using an N-terminal gamma-carboxyglutamic acid domain. A recent report has demonstrated that the GAS6 gene can promote fat deposition and is associated with an increased number of fat cells in mice. In order to.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.39
- DOI:
- 10.4238/2015.December.21.39
Increased survivin expression and its association with clinical parameters of congenital choledochal cysts
Authors: Z.G. Wu, G.X. Li, B. Wang, Z.M. Chen and Q. Feng
The aim of the current study was to investigate survivin expression in congenital choledochal cysts (CCCs), and its associations with clinical parameters of CCCs. In total, 121 children with CCCs were included in this study as the case group, and their cysts were staged according to the Todani classification system. Additional.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028032
- DOI:
- 10.4238/gmr.15028032
Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein
Authors: Y. Yi, Y.-Y. Nian, H.-W. Ji, H. Zhang, L. Zhu and Z.-W. Xu
To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriaceti.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.14.1
- DOI:
- 10.4238/2013.June.14.1
Selecting key genes associated with osteosarcoma based on a differential expression network
Authors: Y.B. Wang, N. Jia, C.M. Xu, L. Zhao, Y. Zhao, X. Wang and T.H. Jia
Despite recent advances in osteosarcoma diagnosis and therapy, much remains unclear about the molecular mechanisms involved in the disorder, and the discovery of novel drug-targeted genes is essential. We explored the potential molecular mechanisms and target genes involved in the development and progression of osteosarcoma. First, we identified the diffe.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.44
- DOI:
- 10.4238/2015.December.21.44
Elevated expression of CXCR4 and correlation with clinicopathological features and prognosis of non-small cell lung cancer patients: a meta-analysis
Authors: H. Wang, F. Xie, Z. Hu and L. Chen
The specific correlation between CXCR4 expression and survival in non-small cell lung cancer (NSCLC) has been investigated independently; however, these have yielded inconsistent results. Therefore, we examined the exact relationship between CXCR4 expression and NSCLC in this meta-analysis. The bibliographic databases in English and Chinese were carefully.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.14
- DOI:
- 10.4238/2015.December.22.14
Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica)
Authors: Z.Q. Yuan, B.S. Zhao and J.Y. Zhang
We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputa.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1042
- DOI:
- 10.4238/vol10-1gmr1042
Hair follicle transcriptome profiles during the transition from anagen to catagen in Cashmere goat (Capra hircus)
Authors: Y.X. Fan, R.B. Wu, X. Qiao, Y.J. Zhang, R.J. Wang, R. Su, J.H. Wu, Y. Dong and J.Q. Li
Previous molecular genetic studies of the goat hair life cycle have focused primarily on a limited number of genes and proteins. To identify additional genes that may play important roles in hair follicle cycle regulation, Illumina sequencing technology was used to catalog differential gene expression profiles in the hair growth cycle (anagen to catagen) .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.15
- DOI:
- 10.4238/2015.December.22.15
Age-dependent expression of the BPI gene in Sutai piglets
Authors: J. Zhu, C. Zi, Z.C. Wu, L. Liu, X.R. Zheng, X.M. Su, G.Q. Zhu, X.G. Huang, S.L. Wu and W.B. Bao
We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18- and 35-day expression in the jejunum. There were also signific.. Read More»
- Genet. Mol. Res. 12(2):
- 2012.April.12.1
- DOI:
- 10.4238/2012.April.12.1
De novo assembly, functional annotation, and marker development of Asian pear (Pyrus pyrifolia) fruit transcriptome through massively parallel sequencing
Authors: J.F. Li, Z. Gao, Y.S. Lou, M. Luo, S.R. Song, W.P. Xu, S.P. Wang and C.X. Zhang
This study investigated the Asian pear transcriptome using the RNA-Seq normalized fruit cDNA library to create a transcriptomic resource for unigene and marker discovery. Following the removal of lowquality reads, 127,085,054 trimmed reads were assembled de novo to yield 37,649 non-redundant unigenes with an average length of 599 bp. Alternative splicing .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.22
- DOI:
- 10.4238/2015.December.23.22