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Expression

Plant Genetics   Research Article

Selection of reference genes for expression analyses of red-fleshed sweet orange (Citrus sinensis)

Authors: T.T. Pinheiro, D.S. Nishimura, F.B. De Nadai, A. Figueira and R.R. Latado

Red-fleshed oranges (Citrus sinensis) contain high levels of carotenoids and lycopene. The growing consumer demand for products with health benefits has increased interest in these types of Citrus cultivars as a potential source of nutraceuticals. However, little is known about the physiology of these cultivars under Brazilian conditions. Transcriptome an.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.32
DOI:
10.4238/2015.December.23.32
Microbial Genetics   Research Article

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Authors: W.Y. Xuan, Y. Zhang, Z.Q. Liu, D. Feng and M.Y. Luo

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. m.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.27
DOI:
10.4238/2015.August.19.27
Animal Genetics   Research Article

Expression pattern of JMJD1C in oocytes and its impact on early embryonic development

Authors: C.H. Li, Y. Gao, S. Wang, F.F. Xu, L.S. Dai, H. Jiang, X.F. Yu, C.Z. Chen, B. Yuan and J.B. Zhang

Cell reprogramming mediated by histone methylation and demethylation is crucial for the activation of the embryonic genome in early embryonic development. In this study, we employed quantitative real-time polymerase chain reaction (qRT-PCR) to detect mRNA levels and expression patterns of all known histone demethylases in early germinal vesicle stage and .. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.12
DOI:
10.4238/2015.December.23.12
Human Genetics   Research Article

Sex- and age-dependent expression of Pax7, Myf 5, MyoG, and Myostatin in yak skeletal muscles

Authors: G. Wu, J. Zhang, L. Wang, S. Xu, J. Zhou, A. Xiang and C. Yang

The aim of this study was to investigate the myogenic factor mRNA expression pattern of Pax7, Myf5, MyoG, and Myostatin mRNA at different ages, sexes, and muscle tissues of Datong yaks. The expression patterns in semimembranosus (SM), quadriceps femoris (QF), and triceps muscle of arm (TM) were detected by quantitative real-ti.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028020
DOI:
10.4238/gmr.15028020
Microbial Genetics   Research Article

Molecular cloning and expression analysis of a pearl oyster (Pinctada martensii) heat shock protein 90 (HSP90)

Authors: H.Y. Liang, Z.X. Wang, Q.N. Lei, R.L. Huang, Y.W. Deng, Q.H. Wang, Y. Jiao and X.D. Du

Heat shock protein 90 (HSP90) is an important molecular chaperone required for proper folding of cellular proteins, and thus, it plays an essential role in protecting cells from damage during stress. In this study, an HSP90 cDNA designated PmHSP90 was cloned from the mantle tissue of the pearl oyster Pinctada martensii using reverse transcription polymera.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.27
DOI:
10.4238/2015.December.28.27
Human Genetics   Research Article

Correlation of increased MALAT1 expression with pathological features and prognosis in cancer patients: a meta-analysis

Authors: X.S. Shi, J. Li, R.H. Yang, G.R. Zhao, H.P. Zhou, W.X. Zeng and M. Zhou

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been identified as a potential cancer biomarker, yet the mechanism by which it influences the development of cancer remains unknown. In this study, we aimed to correlate MALAT1 expression with pathological features and prognosis in cancer patients. Several databases were searched using co.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.30
DOI:
10.4238/2015.December.28.30
Medical Genetics   Research Article

Expression patterns of melatonin receptors in chicken ovarian follicles affected by monochromatic light

Authors: D.Y. Li, N. Wu, J.B. Tu, Y.D. Hu, M.Y. Yang, H.D. Yin, B.L. Chen, H.L. Xu, Y.F. Yao and Q. Zhu

Artificial illumination is an important exogenous factor in the control of many physiological and behavioral processes as well as an important environmental factor in the management of laying hens. Melatonin receptors are members of the G protein-coupled receptor family. The hormone melatonin is secreted primarily by the pineal gland, with highest levels .. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.14
DOI:
10.4238/2015.August.21.14
Bioinformatics   Research Article

Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana)

Authors: T. Ji, F. Shen, Z. Liu, L. Yin, J. Shen, Q. Liang and Y.X. Luo

The mite (Varroa destructor) has become the greatest threat to apiculture worldwide. As the original host of the mite, Apis cerana can effectively resist the mite. An increased understanding of the resistance mechanisms of Eastern honeybees against V. destructor may help researchers to protect other species against these parasites. In this study, the prot.. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.18
DOI:
10.4238/2015.August.21.18
Plant Genetics   Research Article

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Authors: Q.N. Lei, Y.Y. Wu, H.Y. Liang, Z.X. Wang, Z. Zheng and Y.W. Deng

Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends techno.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028799
DOI:
10.4238/gmr.15028799
Microbial Genetics   Research Article

Differential expression of TLP, ERF1, and R2R3MYB in annual Medicago species under salinity conditions

Authors: F. Gharaghani, F. Rafiei, N. Mirakhorli and E. Ebrahimie

The present study was conducted to evaluate the responses of three annual Medicago species (M. truncatula, M. laciniata, and M. polymorpha) to salinity. We analyzed publicly available microarray data in NCBI pertaining to salinity-response genes in M. truncatula. Our data search identified Tubby C2 (TLP) and ethylene responsive transcription factor 1 (ERF.. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.22
DOI:
10.4238/2015.August.21.22