Expression
Association between adipose tissue expression and serum levels of leptin and adiponectin in women with polycystic ovary syndrome
Authors: S.B. Lecke, D.M. Morsch and P.M. Spritzer
We reviewed emerging evidence linking serum levels and adipose tissue expression of leptin and adiponectin in women with polycystic ovary syndrome (PCOS). Previous data obtained by our group from a sample of overweight/obese PCOS women and a control sample of normal weight controls, both stratified by BMI, were reanalyzed. Circulating levels of leptin and.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.16
- DOI:
- 10.4238/2013.February.28.16
Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear
Authors: J. Tang, J. Lin, B.L. Zhang, Z.H. Wang, X.G. Li and Y.H. Chang
A 1794-bp cDNA fragment was amplified from mRNA isolated from pear (Pyrus pyrifolia NaKai. Cuiguan) leaves by using primers based on the sequences generated during the analysis of the pear transcriptome. The 597-amino acid sequence encoded by the cDNA was compared with the sequences in GenBank, and it was found to be similar to that of members of the sucr.. Read More»
- Genet. Mol. Res. 13(4):
Study on the treatment of the p15 gene combined with Bcr-abl-specific siRNA and STI571 for chronic myeloid leukemia
Authors: W. Wang, Y. Du, N.-N. Li, F.-F. Lv and G.-Q. Lin
The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDN.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.4
Expression and purification of GST-FHL2 fusion protein
Authors: H. Yu, Q. Ma, J. Lin, Y.F. Sun and F. Zheng
Escherichia coli is the most widely used host for the production of recombinant proteins. However, most eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. The interactions between human FHL2 protein and many types of proteins, including structural proteins, kinases, and several class.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.6.4
- DOI:
- 10.4238/2013.December.6.4
Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine
Authors: Y.X. Fan, C.H. Gu, Y.L. Zhang, B.S. Zhong, L.Z. Wang, Z.R. Zhou,Z.Y. Wang, R.X. Jia, F. Wang
Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.2.5
- DOI:
- 10.4238/2013.December.2.5
Predicting the target genes of microRNA based on microarray data
Authors: B. Cao, T. Ji, B. Zhou, J. Zou, G.Q. Jiao
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides in length, which play important roles in regulating gene expression post-transcriptionally. Several computational methods and algorithms have been developed to predict miRNA targets. In this study, we described a method that can be used to integrate m.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.2.4
- DOI:
- 10.4238/2013.December.2.4
Analysis of differentially expressed genes in various stages of Duchenne muscular dystrophy by using a network view
Authors: D. Wang, Z.J. Wang, X.X. Song, L.H. Pu, X. Li and Y. Wang
Duchenne muscular dystrophy (DMD), which is caused by mutations in the X-linked dystrophin gene, is a severe and progressive neuromuscular disease with no available cure. By integrating 2 microarray datasets from the Gene Expression Omnibus, we identified differentially expressed genes in 2 stages of DMD and systematically explored their potential disease.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.13
- DOI:
- 10.4238/2013.October.10.13
Cloning and characterization of major histocompatibility complex class II genes in the stone flounder Kareius bicoloratus (Pleuronectidae)
Authors: Cloning and characterization of major histocompatibility complex class II genes in the stone flounder Kareius bicoloratus (Pleuronectidae)
Major histocompatibility complex (MHC) class II genes play important recognition roles in the immune system in vertebrates. We cloned the MHC class II genes A and B in the stone flounder (Kareius bicoloratus). The full-length cDNA and DNA sequences of both genes were obtained, and their characteristic motifs were analyzed. The DNA sequence of stone flound.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.9
- DOI:
- 10.4238/2013.November.22.9
Expression pattern of the zona pellucida 3 (ZP3) gene during ovarian development and the location of ZP3 protein in oocytes in a natural, wild triploid crucian carp mutant, Carassius auratus var. Pingxiangnensis
Authors: J.W. Shi1, J.Q. Sheng1, K. Peng1, J.H. Wang1, W.J. Yi1, H.J. Wu2, Q. Gu1 and Y.J. Hong1,3
Carassius auratus var. Pingxiangnensis (designated CaP), distributed in the Pingxiang region of Jiangxi Province, China, is a natural, wild triploid crucian carp mutant that has two reproductive development modes: gynogenesis and bisexual reproduction. Little information is available about the expression pattern of the zona pellucida 3 (ZP3) gene during o.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.13
- DOI:
- 10.4238/2013.November.18.13
Gene expression during induced differentiation of sheep bone marrow mesenchymal stem cells into osteoblasts
Authors: Y. Hu, X.-X. Tang and H.-Y. He
The aim of this study was to observe the dynamic expression of osteopontin, type I collagen, and osteocalcin genes during the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts and confirm whether BMSCs can differentiate and mature into osteoblasts. A healthy, 2-month-old Altay tailed sheep was obtained, and 10 mL bone marrow was .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.4
- DOI:
- 10.4238/2013.December.11.4