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Expression

Plant Genetics   Research Article

Comparative analysis of polygalacturonase in the fruit of strawberry cultivars

Authors: H.C. Zhou, G. Li, X. Zhao and L.J. Li

The role of polygalacturonase (PG) in the development, ripening, and softening of fruit from two strawberry cultivars with different flesh firmness and softening characteristics was compared. Changes in PG activity and gene expression during development, ripening and softening were measured. The PG genes from each cultivar wer.. Read More»

Genet. Mol. Res. 14(4):
2015.October.19.21
DOI:
10.4238/2015.October.19.21
Plant Genetics   Research Article

Cloning and characterization of up-regulated HbSINA4 gene induced by drought stress in Tibetan hulless barley

Authors: H.J. Yuan, X.M. Luo, T.S. Nyima, Y.L. Wang, Q.J. Xu and X.Q. Zeng

Hulless barley is an important crop cereal in Tibetan, China. Drought is a major abiotic stress in barley production. In this study, we cloned the drought-related HbSINA4 gene from the variety ‘Himalaya 10’ and analyzed its expression patterns under different drought and rehydration conditions. The cDNA of.. Read More»

Genet. Mol. Res. 14(4):
2015.November.30.7
DOI:
10.4238/2015.November.30.7
Plant Genetics   Research Article

Isolation and expression analysis of four HD-ZIP III family genes targeted by microRNA166 in peach

Authors: C.H. Zhang, B.B. Zhang, R.J. Ma, M.L. Yu, S.L. Guo and L. Guo

MicroRNA166 (miR166) is known to have highly conserved targets that encode proteins of the class III homeodomain-leucine zipper (HD-ZIP III) family, in a broad range of plant species. To further understand the relationship between HD-ZIP III genes and miR166, four HD-ZIP III family genes (PpHB14, PpHB15, PpHB8, and PpREV) were isolated from peach (Prunus .. Read More»

Genet. Mol. Res. 14(4):
2015.October.29.37
DOI:
10.4238/2015.October.29.37
Animal Genetics   Research Article

Molecular characterization, chromosome mapping, and expression profile of porcine CDC16

Authors: D. Xu, J. Jiang and H. Ma

Cell division cycle16 (CDC16) is a core component among the eight protein subunits of the anaphase-promoting complex (APC). APC is a cyclin degradation system that governs the exit of cells from mitosis. Not much information is available for CDC16 in pig. In this study, a 2284-bp cDNA of porcine CDC16 was obtained by rapid amp.. Read More»

Genet. Mol. Res. 14(4):
2015.October.19.25
DOI:
10.4238/2015.October.19.25
Animal Genetics   Research Article

Molecular characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep

Authors: Y.X. Li, J. Zhang, Y. Qian, C.H. Meng, H.L. Wang, X.J. Tao, S. Zhong, S.X. Cao and Q.F. Li

NR5A2 has been implicated in processes as diverse as steroidogenesis, cellular proliferation, ovarian follicular development, ovulation, and fertility in mammals. However, data about the relationship between NR5A2 and prolificacy in mammals are lacking. In the present study, we identified and characterized NR5A2 of Hu sheep, a.. Read More»

Genet. Mol. Res. 14(4):
2015.October.19.20
DOI:
10.4238/2015.October.19.20
Animal Genetics   Research Article

Effect of luteolin on gene expression in mouse H22 hepatoma cells

Authors: J.X. Niu1, H.P. Guo2, H.M. Gan3, L.D. Bao4, J.J. Ren1

The purpose of our study was to observe the effects of luteolin on the expression of the genes ICAM-1, LFA-3, and PCNA in H22 hepatoma tissue. Sixty ICR (Institute of Cancer Research) mice with H22 hepatoma were randomly divided into five groups: a normal saline control group, low-, medium-, and high-dose luteolin groups, and a cyclophosphamide group. The.. Read More»

Genet. Mol. Res. 14(4):
Animal Genetics   Research Article

PPAR�± signal pathway gene expression is associated with fatty acid content in yak and cattle longissimus dorsi muscle

Authors: W. Qin, C.N. Liang, X. Guo, M. Chu, J. Pei, P.J. Bao, X.Y. Wu, T.K. Li and P. Yan

ntramuscular fatty acid (FA) is related to meat qualities such as juiciness, tenderness, palatability, and shear force. PPARα plays an important role in lipid metabolism in the liver and skeletal muscle. This study investigated FA composition in yaks and cattle, in order to ascertain whether a correlation between PPARα signal pathway genes as .. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.9
DOI:
10.4238/2015.November.18.9
Plant Genetics   Research Article

Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain

Authors: L.J. Zhang1,3, W.X. Dong1, S.M. Guo1, Y.X. Wang2, A.D. Wang1 and X.J. Lu3

This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The full-length sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively. Multiple alignments of amino acid sequences .. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.21
DOI:
10.4238/2015.November.18.21
Plant Genetics   Research Article

Cloning and expression of the sucrose transporter gene PsSUT1 from tree peony leaf

Authors: Y.H. Li, T. Guo, Y. Cui , Y. Li and D. He

This study reports the cloning of a sucrose transporter gene, PsSUT1, from the leaf of tree peony (Paeonia suffruticosa Lind. cv ‘Huhong’). Expression patterns were examined in different organs and at different developmental stages. The full-length cDNA of PsSUT1 consisted of a 2001-bp sequence containing a 1557-bp.. Read More»

Genet. Mol. Res. 14(4):
Y.H. Li*, T. Guo*, Y. Cui , Y. Li and D. He
DOI:
Y.H. Li*, T. Guo*, Y. Cui , Y. Li and D. He
Plant Genetics   Research Article

Molecular cloning, characterization and expression analysis of WAG-1 in the pistillody line of common wheat

Authors: Q.H. Wang, Z.J. Yang, S.H. Wei, Z.Y. Jiang, Y.F. Yang, Z.S. Hu,Q.X. Sun and Z.S. Peng

Wheat WAG-1 is a C-class MADS-box gene, which is orthologous to AGAMOUS in Arabidopsis. In this study, we report the cloning, characterization, and expression patterns of WAG-1 in the pistillody mutant HTS-1 and its sib-line CSTP. The cDNA of WAG-1 was found to be 765 bp in length, which was equal to the length of its open rea.. Read More»

Genet. Mol. Res. 14(4):
2015.October.16.12
DOI:
10.4238/2015.October.16.12