Gene Expression
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions
Authors: T.J. Nakayama, F.A. Rodrigues, N. Neumaier, F.C. Marcelino-Guimar�£es, J.R.B. Farias, M.C.N. de Oliveira, A. Bor�©m, A.C.B. de Oliveira, B.M. Emygdio and A.L. Nepomuceno
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantifi cation is an effective strategy .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.13.4
- DOI:
- 10.4238/2014.February.13.4
Identification and analysis of the jnk1 gene in polyploid hybrids of red crucian carp (Carassius auratus red var.) and common carp (Cyprinus carpio L.)
Authors: Y.M. Xiao, M.G. Jiang, Z.W. Luo, Y.H. Zhou, S. Wen, M. Wang,C. Zhang and S.J. Liu
c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase superfamily. The allotetraploid crucian carp is a product of distant hybridization of female red crucian carp with male common carp. It is the first natural case of an allotetraploid with stable genetic characters, including fertility .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.1
- DOI:
- 10.4238/2014.February.19.1
Aluminum triggers broad changes in microRNA expression in rice roots
Authors: J.C. Lima, R.A. Arenhart, M. Margis-Pinheiro and R. Margis
MicroRNAs are small 21-nucleotide RNA molecules with regulatory roles in development and in response to stress. Expression of some plant miRNAs has been specifically associated with responses to abiotic stresses caused by cold, light, iron, and copper ions. In acid soils, aluminum solubility increases, thereby causing severe d.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.10.4
- DOI:
- 10.4238/2011.November.10.4
Establishing references for gene expression analyses by RT-qPCR in Theobroma cacao tissues
Authors: T.T. Pinheiro, C.G. Litholdo Jr., M.L. Sereno, G.A. Leal Jr., P.S.B. Albuquerque and A. Figueira
Lack of continuous progress in Theobroma cacao (Malvaceae) breeding, especially associated with seed quality traits, requires more efficient selection methods based on genomic information. Reverse transcript quantitative PCR (RT-qPCR) has become the method of choice for gene expression analysis, but relative expression analysi.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.17.4
- DOI:
- 10.4238/2011.November.17.4
Semi-quantitative detection of gene expression using bisbenzimide dye
Authors: P. Kittimongkolsuk, T. Tencomnao, R. Santiyanont
An electrochemical biosensor, using a disposable electrochemical printed chip aggregation by the bisbenzimide dye (Hoechst 33258), was used for detecting the expression of b-actin and RAGE genes. Using linear sweep voltammetry, the expression of these two genes in HeLa and HepG2 cell lines was determined based on anodic peak c.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.22.8
- DOI:
- 10.4238/2011.November.22.8
Long-term effects of evodiamine on expressions of lipogenesis and lipolysis genes in mouse adipose and liver tissues
Authors: D.F. Jiang, W.T. Li2, H.L. Yang, Z.Z. Zhang, D. Chen and C. Sun
Evodiamine, the major alkaloid component isolated from the fruit of dried, unripened Evodia rutaecarpa Bentham, affects the plasma levels of cholecystokinin and various biological events such as gastric emptying and gastrointestinal transit; these effects of evodiamine were previously investigated in male rats. In this study, .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.20.5
- DOI:
- 10.4238/2014.February.20.5
IFGFA: Identification of featured genes from genomic data using factor analysis
Authors: C.H. Fu, S. Deng, J.H. Wu, X.Q. Wu, Z.H. Fu and Z.G. Yu
In this study, a software tool (IFGFA) for identification of featured genes from gene expression data based on latent factor analysis was developed. Despite the availability of computational methods and statistical models appropriate for analyzing special genomic data, IFGFA provides a platform for predicting colon cancer-related genes and can be applied .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038803
- DOI:
- 10.4238/gmr.15038803
Meta-analysis of differentially expressed genes in ankylosing spondylitis
Authors: Y.H. Lee and G.G. Song
The purpose of this study was to identify differentially expressed (DE) genes and biological processes associated with changes in gene expression in ankylosing spondylitis (AS). We performed a metaanalysis using the integrative meta-analysis of expression data program on publicly available microarray AS Gene Expression Omnibus (GEO) datasets. We performed.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.6
- DOI:
- 10.4238/2015.May.18.6
Expression profiles of genes associated with mitochondria-mediated apoptosis and their roles in liver regeneration
Authors: X.K. Xing, M.H. Li, Z.W. Guo and C.S. Xu
Mitochondria are closely associated with cell survival, and it is of interest to determine whether apoptosis pathways, which are mediated by mitochondria, are involved in liver regeneration (LR). To identify the mechanisms underlying mitochondria-mediated apoptosis during rat LR, we used the Rat Genome 230 2.0 Array to investigate changes in gene expressi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038101
- DOI:
- 10.4238/gmr.15038101
Confined housing system increased abdominal and subcutaneous fat deposition and gene expressions of carbohydrate response element-binding protein and sterol regulatory element-binding protein 1 in chicken
Authors: Q. Li, X.L. Zhao, E.R. Gilbert, Y.P. Liu, Y. Wang, M.H. Qiu and Q. Zhu
Free-range production system is increasingly being used in poultry breeding and feed production in many countries. The objective of the current experiment was to evaluate the effects of different raising systems on fat-related traits and mRNA levels of liver lipogenesis genes in Erlang Mountainous chicken. Each of 10 birds (91 day old) from caged, indoor-.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.24
- DOI:
- 10.4238/2015.February.6.24