Gene Expression
Cloning of an anthocyanidin synthase gene homolog from blackcurrant (Ribes nigrum L.) and its expression at different fruit stages
Authors: X.-G. Li, J. Wang and Z.-Y. Yu
Anthocyanidin synthase (ANS), a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase, catalyzes the penultimate step in anthocyanin biosynthesis, from leucoanthocyanidins to anthocyanidins, the first colored compound in the anthocyanin pathway. In this study, a full-length, 1427-bp long cDNA named RnANS1, which is homologous to the anthocyanidin synthase g.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.2
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.2
Cloning and expression analysis of heat-shock transcription factor gene CaHsfA2 from pepper (Capsicum annuum L.)
Authors: M. Guo, Y.-X. Yin, J.-J. Ji, B.-P. Ma, M.-H. Lu, and Z.-H. Gong,
The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends. The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.14
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.14
Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology
Authors: S. Honsawek, P. Bumrungpanichthaworn, T. Thitiset and L. Wolfinbarger Jr.
Demineralized bone matrix (DBM) has been widely investigated as a biomaterial to promote new bone formation and is utilized clinically for bone repair and regeneration. We investigated gene expression patterns of osteogenic differentiation in human periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA arra.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1329
- DOI:
- 10.4238/vol10-3gmr1329
Circulatory microRNA-145 expression is increased in cerebral ischemia
Authors: C.S. Gan, C.W. Wang and K.S. Tan
Cerebral ischemia or ischemic stroke is mainly attributed to vascular and circulation disorders. Among protein biomarkers, RNA profiles have also been identified as markers of ischemic stroke. MicroRNA-145 expression is ostensibly recognized as marker and modulator of vascular smooth muscle cell phenotype; however, expression .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.27.1
- DOI:
- http://dx.doi.org/10.4238/2012.January.27.1
Support vector machines for novel class detection in Bioinformatics
Authors: Eduardo J. Spinosa and Andr�© C.P.L.F. de Carvalho
Novelty detection techniques might be a promising way of dealing with high-dimensional classification problems in Bioinformatics. We present preliminary results of the use of a one-class support vector machine approach to detect novel classes in two Bioinformatics databases. The results are compatible with theory and inspire f.. Read More»
- Genet. Mol. Res. 4(3):
Gene selection based on multi-class support vector machines and genetic algorithms
Authors: Bruno Feres de Souza and Andr�© Ponce de Leon F. de Carvalho
Microarrays are a new technology that allows biologists to better understand the interactions between diverse pathologic state at the gene level. However, the amount of data generated by these tools becomes problematic, even though data are supposed to be automatically analyzed (e.g., for diagnostic purposes). The issue become.. Read More»
- Genet. Mol. Res. 4(3):
Position effect variegation and epigenetic modification of a transgene in a pig model
Authors: Z. Yin, Q.R. Kong, Z.P. Zhao, M.L. Wu, Y.S. Mu, K. Hu1 and Z.H. Liu
Sequences proximal to transgene integration sites are able to regulate transgene expression, resulting in complex position effect variegation. Position effect variegation can cause differences in epigenetic modifications, such as DNA methylation and histone acetylation. However, it is not known which factor, position effect or.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.16.1
- DOI:
- 10.4238/2012.February.16.1
Differential gene expression and functional analysis of pit cells from regenerating rat liver
Authors: C.S. Xu, X.G. Chen, C.F. Chang, G.P. Wang, W.B. Wang, L.X. Zhang, Q.S. Zhu and L. Wang
Hepatic pit cells are a population of large granular lymphocytes that substantially contribute to hepatic immunity. Studies have proven that pit cells have a role in liver regeneration, but the details of the relationship between pit cells and liver regeneration is not clear at present. We subjected rats to a two-third hepatectomy; pit cells with high pur.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1052
- DOI:
- 10.4238/vol10-2gmr1052
Detection of differentially expressed genes in the longissimus dorsi of Northeastern Indigenous and Large White pigs
Authors: Y. Gao, Y.H. Zhang, H. Jiang, S.Q. Xiao, S. Wang, Q. Ma, G.J. Sun, F.J. Li, Q. Deng, L.S. Dai, Z.H. Zhao, X.S. Cui, S.M. Zhang, D.F. Liu and J.B. Zhang
Recent attention in pig breeding programs has focused on the improvement of pork quality in response to increasing consumer demands. Compared to the fatty-type Northeastern Indigenous (Chinese) breed of pigs, the lean-type Large White has lower intramuscular fat and inferior eating quality from the perspective of the Chinese c.. Read More»
- Genet. Mol. Res. 10(2):
Devolopmental and growth temperature regulation of omega-3 fatty acid desaturase genes in safflower (Carthamus tinctorius L.)
Authors: L.-L. Guan, W. Wu, B. Hu, D. Li, J.-W. Chen, K. Hou and L. Wang
Three ω-3 fatty acid desaturase genes (CtFAD3, CtFAD7, and CtFAD8) were isolated from safflower (Carthamus tinctorius L.). Transcript analysis showed that the highest transcript levels were detected for CtFAD3 and the low transcript levels were detected for CtFAD7 and.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.7
- DOI:
- 10.4238/2014.August.28.7