Gene Expression
Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines
Authors: J. Ogorevc and P. Dov?
Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.12
Effects of the antipsoriatic drug dithranol on E2A and caspase-9 gene expression in vitro
Authors: C. Ronpirin and T. Tencomnao
Although the precise causes of psoriasis remain to be elucidated, psoriasis has been known as a disorder in which factors in the immune system, enzymes and other biochemical substances that regulate skin cell division are functionally imbalanced, thereby resulting in rapid proliferation of keratinocytes and incomplete keratini.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.17.3
- DOI:
- 10.4238/2012.February.17.3
Molecular characterization and expression pattern of an ?±-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii
Authors: Molecular characterization and expression pattern of an ?±-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii
The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.10
- DOI:
- 10.4238/2014.August.28.10
Isolation and partial characterization of a root-specific promoter for stacking multiple traits into cassava (Manihot esculenta CRANTZ)
Authors: M.A. Gbadegesin and J.R. Beeching
Cassava can be cultivated on impoverished soils with minimum inputs, and its storage roots are a staple food for millions in Africa. However, these roots are low in bioavailable nutrients and in protein content, contain cyanogenic glycosides, and suffer from a very short post-harvest shelf-life, and the plant is susceptible to.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr892
- DOI:
- 10.4238/vol10-2gmr892
Gene expression in the lignin biosynthesis pathway during soybean seed development
Authors: A. Baldoni, E.V.R. Von Pinho, J.S. Fernandes, V.M. Abreu and M.L.M. Carvalho
The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are sca.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.February.28.2
- DOI:
- 10.4238/2013.February.28.2
Regulation of WNK4 gene transcription in the kidneys
Authors: Z.J. Sun, Y. Li, Y.H. Li, J.S. Zhang, Q. Ding, J. Li-Ling, Y. Liang and Y.Y. Zhao
With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoaldosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mech.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.9
- DOI:
- 10.4238/2013.January.4.9
Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions
Authors: I. Aquino, M.S.F. Tsuboy, J.C. Marcarini, M.S. Mantovani, F.F. Perazzo and E.L. Maistro
The malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to eval.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.24.6
- DOI:
- 10.4238/2013.July.24.6
Cloning of three genes involved in the flavonoid metabolic pathway and their expression during insect resistance in Pinus massoniana Lamb.
Authors: Z.Q. Yang, H. Chen, J.H. Tan, H.L. Xu, J. Jia and Y.H. Feng
Pinus massoniana Lamb. is an important timber and turpentine-producing tree species in China. Dendrolimus punctatus and Dasychira axutha are leaf-eating pests that have harmful effects on P. massoniana production. Few studies have focused on the molecular mechanisms underlying pest resistance in P. massoniana. Based on sequenc.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049332
- DOI:
- 10.4238/gmr15049332
Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.)
Authors: J. Zhang, X.L. Sun, L.G. Zhang, M.X. Hui and M.K. Zhang
Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.24.5
- DOI:
- 10.4238/2013.July.24.5
Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression
Authors: W. Gao, W. Sun, R. Su, X.Y. Lv, Q.Z. Wang, D. Li, H.H. Musa, L. Chen, H. Zhou, H.S. Xu and W.H. Hua
RT-PCR was used to study the temporal and spatial pattern of Yes-associated protein 1 (YAP1) and myosin heavy chain (MyHC) expression in four different skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and three growth stages (i.e., 2 days old, 2 and 6 months .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027260
- DOI:
- 10.4238/gmr.15027260