Gene Expression

Molecular characterization and expression analysis of the �²-actin gene from the ridgetail white prawn Exopalaemon carinicauda

Authors: J.P. Liang, Y. Wang, Q.Q. Ge, J.T. Li, P. Liu, J. Li and G.X. Nie

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was .. Read More»

Genet. Mol. Res. 15(2):

gmr.15026872

DOI:

10.4238/gmr.15026872

Relationship between TLR4 and MCP2 expression levels and habitual abortion

Authors: X.P. Li, L.N. Song, L.P. Tian and Y.S. Zhang

Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression .. Read More»

Genet. Mol. Res. 15(2):

gmr.15027378

DOI:

10.4238/gmr.15027378

Chicken skeletal muscle-associated macroarray for gene discovery

Authors: E.C. Jorge, C.M.R. Melo, M.F. Ros�¡rio, J.R.S. Rossi, M.C. Ledur, A.S.A.M.T. Moura and L.L. Coutinho

Macro- and microarrays are well-established technologies to determine gene functions through repeated measurements of transcript abundance. We constructed a chicken skeletal muscle-associated array based on a muscle-specific EST database, which was used to generate a tissue expression dataset of ~4500 chicken genes across 5 ad.. Read More»

Genet. Mol. Res. 9(1):

vol9-1gmr712

DOI:

10.4238/vol9-1gmr712

Microarray analysis of differentially expressed genes in the liver between Bai�er layers and broilers

Authors: Q.-G. Wang , H.-F. Zhang, S.-Z. Wang, G.L. Gao, L. Leng, W. Na and H. Li

In this study, we profiled gene expression in chicken liver and screened differentially expressed genes in the Bai’er layers and fat line broilers. Birds were derived from the 14th generation of Northeast Agricultural University fat broiler lines and Bai’er layers. Chicken genome arrays were used to screen differentially expressed genes in liv.. Read More»

Genet. Mol. Res. 14(1):

2015.March.31.19

DOI:

10.4238/2015.March.31.19

Biochemical and molecular studies of copper metabolism during the infection process of the human pathogenic fungus Paracoccidioides brasiliensis

Authors: R.S. Santos

Genet. Mol. Res. 9(1):

vol9-1TA028

DOI:

10.4238/vol9-1TA028

Detection and expression of bovine papillomavirus in blood of healthy and papillomatosis-affected cattle

Authors: M.A.R. Silva, B.M.F. De Albuquerque, N.E. Pontes, L.C.A. Coutinho, M.C.G. Leit�£o, M.C. Reis, R.S. Castro and A.C. Freitas

Papillomaviruses (PV) are double-stranded DNA viruses that can cause benignant and malignant tumors in amniotes. There are 13 types of bovine papillomavirus (BPV-1 to -13); they have been found in reproductive tissues and body fluids. Normally these viruses are detected in epithelial tissue. We looked for BPV in the blood of healthy cattle and cattle with.. Read More»

Genet. Mol. Res. 12(3):

2013.February.28.31

DOI:

10.4238/2013.February.28.31

Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits

Authors: T.S. Xu, L.H. Gu, X.H. Zhang, B.G. Ye, X.L. Liu and S.S. Hou

Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation. To identify the characteristics of the Pekin duck MSTN gene and the relationship between its polymorphism and breast muscle traits in Pekin duck, cDNA cloning an.. Read More»

Genet. Mol. Res. 12(3):

2013.February.28.18

DOI:

10.4238/2013.February.28.18

Effects of tail docking on the expression of genes related to lipid metabolism in Lanzhou fat-tailed sheep

Authors: Y. Yue, X. Cheng, S.G. Zhao, Z. Liu, L.S. Liu, R. Zhou, J.P. Wu and M.A. Brown

To evaluate stearoyl-CoA desaturase (SCD), hormone-sensitive lipase (HSL), lipoprotein lipase (LPL), and peroxisome proliferator-activated receptor (PPARγ) expression in Lanzhou fat-tailed sheep (with and without docked tails), 18 rams were randomly divided into two equal groups (docked group, LT; control group, LC). The.. Read More»

Genet. Mol. Res. 15(1):

gmr.15017323

DOI:

10.4238/gmr.15017323

Roles of functional NFKB1 and �²-TrCP insertion/deletion polymorphisms in mRNA expression and epithelial ovarian cancer susceptibility

Authors: Z.H. Huo, H.J. Zhong, Y.S. Zhu, B. Xing and H. Tang

Epithelial ovarian cancer (EOC) is the leading cause of death among all gynecological cancers. Nuclear factor-kappa B (NF-κB) is involved in carcinogenesis and in the development of EOC. The β-transducin repeat-containing protein (β-TrCP) is a positive regulator of the NF-κB signaling pathway. Recent studies have indicated that the -.. Read More»

Genet. Mol. Res. 12(3):

2013.March.11.6

DOI:

10.4238/2013.March.11.6

Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)

Authors: Y.-L. Zhang, D.-W. Li, Z.-H. Gong, J.-E. Wang, Y.-X. Yin and J.-J. Ji

Based on culture isolation and morphological observation blight-infected pepper plants in Shaanxi Province, China, we identified the pathogen causing pepper phytophthora blight as Phytophthora capsici. Varieties that differed in resistance (CM334, PBC602, and B27) were inoculated with this pathogen. The root activity of resistant CM334 variety was the hig.. Read More»

Genet. Mol. Res. 12(3):

2013.September.13.5

DOI:

10.4238/2013.September.13.5