Gene Expression
Identification and expression analysis of YABBY family genes associated with fruit shape in tomato (Solanum lycopersicum L.)
Authors: H.Q. Han, Y. Liu, M.M. Jiang, H.Y. Ge and H.Y. Chen
YABBY family genes play important roles in the development of leaf, flower, and fruit. The purpose of this research was to integrate all the YABBY genes and analyze the correlation between gene expression and fruit shape in tomato. Scanning of 24 genomes of sequenced species demonstrated that YABBY genes were very normal and stable in flowering plants exc.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.1
- DOI:
- 10.4238/2015.June.29.1
Molecular cloning and tissue expression analyses of two novel pepper genes: heterotrimeric G protein beta 2 subunit and ArcA1
Authors: M.H. Deng, J.L. Huo, H.S. Zhu, H. Zhou and J.F. Wen
We isolated two transcription factor genes, heterotrimeric G protein beta 2 subunit (Gβ2) and ArcA1, from pepper (Capsicum annuum). The complete coding sequences were amplified using reversed transcriptase PCR based on conserved sequence information of Solanum lycopersicum and several other plant species. Nucleotide sequence analysis of these two gen.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.1
- DOI:
- 10.4238/2013.April.17.1
Genome-wide analysis of TCP family in tobacco
Authors: L. Chen, Y.Q. Chen, A.M. Ding, H. Chen, F. Xia, W.F. Wang and Y.H. Sun
The TCP family is a transcription factor family, members of which are extensively involved in plant growth and development as well as in signal transduction in the response against many physiological and biochemical stimuli. In the present study, 61 TCP genes were identified in tobacco (Nicotiana tabacum) genome. Bioinformatic.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027728
- DOI:
- 10.4238/gmr.15027728
Screening and analyzing genes associated with Amur tiger placental development
Authors: Q. Li, T.F. Lu, D. Liu, P.F. Hu, B. Sun, J.Z. Ma, W.J. Wang, K.F. Wang, W.X. Zhang, J. Chen, W.J. Guan, Y.H. Ma and M.H. Zhang
The Amur tiger is a unique endangered species in the world, and thus, protection of its genetic resources is extremely important. In this study, an Amur tiger placenta cDNA library was constructed using the SMART cDNA Library Construction kit. A total of 508 colonies were sequenced, in which 205 (76%) genes were annotated and .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.25
- DOI:
- 10.4238/2014.September.26.25
Dietary glycerol for quail: association between productive performance and COX III mRNA expression
Authors: S.C.C. Silva, E. Gasparino, E. Batista, F. Tanamati, A.P.D. Vesco, B. Lala and D.P. de Oliveira
This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake wer.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027435
- DOI:
- 10.4238/gmr.15027435
Deep sequencing-based analysis of gene expression in bovine mammary epithelial cells after Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae infection
Authors: L. Xiu, Y.B. Fu, Y. Deng, X.J. Shi, Z.Y. Bian, A. Ruhan and X. Wang
The goal of this study was to characterize the transcriptome of primary bovine mammalian epithelial cells (pBMECs) and to identify candidate genes for response and resistance to Staphylococcus aureus (strain S108), Escherichia coli (strain E23), and Klebsiella pneumoniae (strain K96) infection. Using Solexa sequencing, approximately 4.9 million total sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.1
- DOI:
- 10.4238/2015.December.15.1
Effect of Fimbristylis ovata on receptor for advanced glycation end-products,proinflammatory cytokines, and cell adhesion molecule level and gene expression in U937 and bEnd.3 cell lines
Authors: S. Sukjamnong and R. Santiyanont
Fimbristylis ovata has been long used as a traditional medicine for chronic inflammatory diseases; however, there are no data regarding its anti-inflammatory properties. In this study, we investigated the effects of F. ovata extracts on the secretion of pro-inflammatory cytokines, cell adhesion molecule, and receptor for advanced glycation end-products (R.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.13
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.13
Cancer classification based on gene expression using neural networks
Authors: H.P. Hu, Z.J. Niu, Y.P. Bai and X.H. Tan
Based on gene expression, we have classified 53 colon cancer patients with UICC II into two groups: relapse and no relapse. Samples were taken from each patient, and gene information was extracted. Of the 53 samples examined, 500 genes were considered proper through analyses by S-Kohonen, BP, and SVM neural networks. Classification accuracy obtained by S-.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.33
- DOI:
- 10.4238/2015.December.21.33
Expression of genes coding for selected amino acid transporters in small intestine, liver, and skeletal muscle of pigs fed excess branched-chain amino acids
Authors: M. Cervantes, N. Arce, H. GarcÃ?Âa, M. Cota, J.K. Htoo and A. Morales
Excess Leu reduces the availability of Ile and Val in pigs likely by reducing absorption of the latter amino acids (AA). Twenty-four crossbred pigs were used to evaluate the effect of excess Leu alone or with surplus Ile and Val on the expression of cationic (b0,+AT and CAT1) and neutral (B0AT1) AA transporters in the small intestine, liver, and skeletal .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.11
- DOI:
- 10.4238/2015.August.19.11
Selection of reference genes in canine uterine tissues
Authors: M. Du, X. Wang, Y.W. Yue, P.Y. Zhou, W. Yao, X. Li, X.B. Ding,X.F. Liu, H. Guo and W.Z. Ma
Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relati.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028138
- DOI:
- 10.4238/gmr.15028138