Gene Expression
De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress
Authors: A.A. Belesini, F.M.S. Carvalho, B.R. Telles, G.M. de Castro, P.F. Giachetto, J.S. Vantini, S.D. Carlin, J.O. Cazetta, D.G. Pinheiro and M.I.T. Ferro
Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16028845
- DOI:
- 10.4238/gmr16028845
Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements
Authors: N. Marini, C.B. Bevilacqua, M.V. B�¼ttow, M.C.B. Raseira and S. Bonow
Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Pru.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029666
- DOI:
- 10.4238/gmr16029666
Association between DLK1 and IGF-I gene expression and meat quality in sheep
Authors: R. Su1, W. Sun1, D. Li1, Q.Z. Wang1, X.Y. Lv1, H.H. Musa2, L. Chen3, Y.F. Zhang3 and W.Z. Wu3
The aim of the present study was to detect delta-like 1 homolog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality... Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.26
- DOI:
- 10.4238/2014.December.4.26
Expression of calpastatin and myostatin genes associated with lamb meat quality
Authors: A. Bagatoli1, E. Gasparino1, M.A.M. Soares2, R.M. Amaral1,F.A.F. Macedo1, D.M. Voltolini1 and A.P. Del Vesco1
Calpastatin (CAST) is an endogenous calpain inhibitor and its main function is to modulate the proteolytic action of enzymes responsible for post-mortem myofibril deterioration. The myostatin gene (GDF-8) acts as a negative regulator of skeletal muscle growth. The expression of these two genes, as well as their interaction, affects the quality of the meat.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.December.4.3
- DOI:
- 10.4238/2013.December.4.3
Screening of the residual normal ovarian tissue adjacent to orthotopic epithelial ovarian carcinomas in nude mice
Authors: G.H. Zhu, S.T. Wang, M.Z. Yao, J.H. Cai, C.Y. Chen, Z.X. Yang, L. Hong and S.Y. Yang
The objective of this study was to explore the feasibility and methods of screening the residual normal ovarian tissue adjacent to orthotopic ovarian carcinomas in nude mice. Human epithelial ovarian cancer cells (OVCAR3) were subcutaneously implanted for a tumor source and ovarian orthotopic transplantation. The cancer tissue.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.6
- DOI:
- 10.4238/2014.April.16.6
Developmental changes in IGF-I and MyoG gene expression and their association with meat traits in sheep
Authors: W. Sun, R. Su1, D. Li, H.H. Musa, Y. Kong, J.T. Ding, Y.H. Ma, L. Chen, Y.F. Zhang and W.Z. Wu
In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.6
- DOI:
- 10.4238/2014.April.14.6
Expression and enzymatic activity of phenylalanine ammonia-lyase and p-coumarate 3-hydroxylase in mango (Mangifera indica �Ataulfo�) during ripening
Authors: H. Palafox-Carlos, C.A. Contreras-Vergara, A. Muhlia-Almaz�¡n, M.A. Islas-Osuna and G.A. Gonz�¡lez-Aguilar
Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar ‘Ataulfo’ in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.10
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.10
Critical evaluation of transcription factor Atf2 as a candidate modulator of alcohol preference in mouse and human populations
Authors: L.S. Wang, Y. Jiao, Y. Huang, X.Y. Liu, G. Gibson, B. Bennett,K.M. Hamre, D.W. Li, H.Y. Zhao, J. Gelernter, H.R. Kranzler,L.A. Farrer, L. Lu, Y.J. Wang, W.K. Gu
In prior work, congenic strains carrying the DBA/2Igb (D2) region of chromosome 2 (Chr2) for alcohol preference were bred onto a C57BL/6Ibg (B6) background and as predicted were found to reduce voluntary consumption. Subsequently, interval-specific congenic recombinant strains (ISCRS) were generated and also tested. These ISCR.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.9
- DOI:
- 10.4238/2013.November.26.9
Changes of gene expression profiles across different phases of vascular calcification in rats
Authors: Z.M. Jiang, X.J. Wu, Y. Liu, X.H. Du, S.J. Shen, L.Y. Xu,W.X. Sun
This study investigated the alteration of gene expression profiles in order to gain a deeper understanding into the molecular mechanism involved in different processes of vascular calcification (VC). Sprague Dawley (SD) rats were injected with 300,000 μg/kg vitamin D3 and gavaged with 25 mg/kg nicotine for 8 or 16 weeks to .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.4
- DOI:
- 10.4238/2013.November.26.4
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
Authors: I.B. Cordeiro1, D.P. Castro7, P.P.O. Nogueira7, P.C.S. Angelo6, P.A. Nogueira7, J.F.C. Gon�§alves8, A.M.R.F. Pereira9, J.S. Garcia2,3, G.H.M.F. Souza2,4, M.A.Z. Arruda5, M.N. Eberlin2, S. Astolfi-Filho1, E.V. Andrade1 and J.L. L�³pez-Lozano10
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.1
- DOI:
- 10.4238/2013.October.29.1