Gene Expression
A new reliable reference gene UBA52 for quantitative real-time polymerase chain reaction studies in pyloric cecal tissues of the starfish Asterias rubens
Authors: A.F. Sadritdinova, A.A. Dmitriev, A.V. Snezhkina, M.S. Belenikin, G.S. Krasnov, O.G. Manylov, A.A. Kudryavtsev, N.V. Melnikova, A.S. Speranskaya, M.V. Darii, V.A. Lakunina, L.A. Uroshlev, A.O. Smurov, O.A. Stepanov and A.V. Kudryavtseva
The starfish Asterias rubens is one of the most abundant echinoderm species in the White, Barents, North, and Baltic Seas.This species is an important component of marine ecosystems and a model object for certain biological studies, in particular those requiring quantitative estimation of gene expression. As a rule, expression.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.8
Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor
Authors: Transcriptional responses in eastern honeybees (Apis cerana) infected with mites, Varroa destructor
The Varroa destructor mite has become the greatest threat to Apis mellifera health worldwide, but rarely causes serious damage to its native host Apis cerana. Understanding the resistance mechanisms of eastern bees against Varroa mites will help researchers determine how to protect other species from this organism. The A. cerana genome has not been previo.. Read More»
- Genet. Mol. Res. 13(4):
Expression of cationic amino acid transporters, carcass traits, and performance of growing pigs fed low-protein amino acid-supplemented versus high protein diets
Authors: A. Morales, F. Grageola, H. GarcÃ?Âa, A. Araiza, R.T. Zijlstra and M. Cervantes
Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.9
- DOI:
- 10.4238/2013.October.18.9
Aberrant gene expression profiles in hepatocellular carcinoma detected by microdissection
Authors: Y.M. Wei1, Y.Y. Li2, Y.C. Zhang2 and Y.Q. Nie2
The aim of this study was to identify genomic aberrations in hepatocellular carcinoma (HCC) by using laser capture microdissection (LCM) combined with microarray analysis. Samples were procured by LCM from HCC and patient-matched normal liver tissue surgically resected from 4 patients. RNA was isolated from the samples and reverse transcribed into cDNA. A.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.3
- DOI:
- 10.4238/2013.November.18.3
Predicting the target genes of microRNA based on microarray data
Authors: B. Cao, T. Ji, B. Zhou, J. Zou, G.Q. Jiao
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides in length, which play important roles in regulating gene expression post-transcriptionally. Several computational methods and algorithms have been developed to predict miRNA targets. In this study, we described a method that can be used to integrate m.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.2.4
- DOI:
- 10.4238/2013.December.2.4
Gene expression during induced differentiation of sheep bone marrow mesenchymal stem cells into osteoblasts
Authors: Y. Hu, X.-X. Tang and H.-Y. He
The aim of this study was to observe the dynamic expression of osteopontin, type I collagen, and osteocalcin genes during the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts and confirm whether BMSCs can differentiate and mature into osteoblasts. A healthy, 2-month-old Altay tailed sheep was obtained, and 10 mL bone marrow was .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.4
- DOI:
- 10.4238/2013.December.11.4
Johanson-Blizzard syndrome: a report of gender-discordant twins with a novel UBR1 mutation
Authors: C.R. Quaio, Y.K. Koda, D.R. Bertola, M. Sukalo, M. Zenker and C.A. Kim
Johanson-Blizzard syndrome (JBS) is a rare autosomal recessive disorder resulting from loss-of-function mutations in the UBR1 gene. JBS can be easily recognized by its unique clinical presentation (including exocrine pancreatic insufficiency, hypoplasia/aplasia of the alae nasi, congenital scalp defects, sensorineural hearing .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.2
Expression of 1alpha-HYD and 24-HYD in bovine kidney mediated by vitamin D3 supplementation
Authors: L.R. Rezende, E.F. Delgado, A.R.L. J�ºnior, G. Gasparin, E.C. Jorge, G.B. Mour�£o and L.L. Coutinho
order to better understand vitamin D3 in cattle metabolism, we quantified 1alpha-HYD and 24-HYD gene expression. In the kidneys of 35 male Nellore cattle, these were divided into a control group and two treatment groups (2 x 106 international units of vitamin D3 administered for 2 or 8 consecutive days pre-slaughter). Vitamin D3 supplementation resulted i.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.12
- DOI:
- 10.4238/2013.December.11.12
Expression analysis of VfDGAT2 in various tissues of the tung tree and in transgenic yeast
Authors: Q.Q. Cui*, Y.C. Chen, X.J. Han, Z.Y. Zhan, L.Y. Lin, L.L. Si and Y.D. Wang
The tung tree (Vernicia fordii Hemsl.; Vf) has great potential as an industrial crop owning to its seed oil that has multiple uses. Diacylglycerol acyltransferases (DGATs) catalyze the last and most committed step of triacylglycerol (TAG) biosynthesis. In order to examine the physiological role of the VfDGAT2 gene in the tung tree, we characterized its ex.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.7
- DOI:
- 10.4238/2013.December.11.7
Gene expression and molecular modeling of the HSP104 chaperone of Trypanosoma cruzi
Authors: R.A. Campos, M.L. da Silva, G.V. da Costa, P.M. Bisch, J.M. Peralta, R. Silva, E. Rondinelli and T.P. ��rm�©nyi
Heat shock protein (HSP) 104 is a highly conserved molecular chaperone that catalyzes protein unfolding, disaggregation and degradation under stress conditions. We characterized HSP104 gene structure and expression in Trypanosoma cruzi, a protozoan parasite that causes Chagas’ disease. The T. cruzi HSP104 is an 869 amino-acid protein encoded by a si.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.15
- DOI:
- 10.4238/2012.August.6.15