Gene Expression
Screening for genes that are differentially-expressed between gastric cancer cells and gastric tumor sphere cells using the gene chip technique
Authors: W.H. Shi, Y.B. Yu, Z.-Y. Li, X.-C. Guo, Z.-L. Wei and Z.-H. Zheng
The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both sa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.54
- DOI:
- 10.4238/2015.November.18.54
Alteration of Phascolosoma esculenta heat shock protein 90 expression under heavy metal exposure and thermal stress
Authors: C.H. Li, X.R. Su, M.Q. Wang, Y.Y. Li, Y. Li, J. Zhou and T.W. Li
The full-length complementary DNA (cDNA) of heat shock protein 90 was cloned from Phascolosoma esculenta (PeHSP90) using expressed sequence tag and rapid amplification of cDNA end approaches. The cDNA of PeHSP90 was 2521 bp including a 5'-untranslated region of 110 bp, a 3'-untranslated region of 230 bp, and an open reading frame of 2181 bp. All of the ch.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.14
- DOI:
- 10.4238/2012.July.10.14
Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape
Authors: X.Q. Huang, R.Q. He, X.Y. Liao, B. Zhou, W.S. Peng, J.Z. Lin, D.Y. Tang, Y.H. Zhu, X.Y. Zhao and X.M. Liu
Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed ra.. Read More»
- Genet. Mol. Res. 13(2):
Boric acid increases the expression levels of human anion exchanger genes SLC4A2 and SLC4A3
Authors: F. Akbas and Z. Aydin
Boron is an important micronutrient in plants and animals. The role of boron in living systems includes coordinated regulation of gene expression, growth and proliferation of higher plants and animals. There are several well-defined genes associated with boron transportation and tolerance in plants and these genes show close h.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.3.6
- DOI:
- 10.4238/2012.April.3.6
Expression of CD44 in sheep oocytes and preimplantation embryos
Authors: J.V. Luz, A.S. Alc�¢ntara-Neto, R.I.T.P. Batista, J.M.G. Souza, D.I.A. Teixeira, L.M. Melo and V.J.F. Freitas
The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35).. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.3.2
- DOI:
- 10.4238/2012.April.3.2
Molecular cloning and expression of the male sterility-related CtYABBY1 gene in flowering Chinese cabbage (Brassica campestris L. ssp chinensis var. parachinensis)
Authors: X.L. Zhang and L.G. Zhang
Expression of the YABBY gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. However, the YABBY gene has not been studied in male sterility and fertility restoration... Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.10.1
- DOI:
- http://dx.doi.org/10.4238/2014.June.10.1
Molecular characteristics and cloning of two pepper genes AN2 and UPA20
Authors: H.X. Chen, H. Jiang, J.L. Huo, J.F. Wen, H.S. Zhu, C.H. Ma, H. Zhou, J.H. Lv and M.H. Deng
The complete coding sequences (CDSs) of “Yunnan Purple Pepper No.1” (Capsicum annuum L.) AN2 and UPA20 genes were amplified using the reverse transcriptase polymerase chain reaction on the basis of the conserved sequence information of some Solanaceae plants and known highly homologous pepper expressed sequence tags. The nucleotide sequence an.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.17.6
- DOI:
- 10.4238/2014.January.17.6
Homology-based analysis of the GRAS gene family in tobacco
Authors: Y.Q. Chen, S.S. Tai, D.W. Wang, A.M. Ding, T.T. Sun, W.F. Wang and Y.H. Sun
Members of the GRAS gene family are important transcriptional regulators. In this study, 21 GRAS genes were identified from tobacco, and were classified into eight subgroups according to the classification of Arabidopsis thaliana. Here, we provide a preliminary overview of this gene family in tobacco, describ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.7
- DOI:
- 10.4238/2015.November.25.7
Effects of exogenous 5-aminolevulinic acid on PIP1 and NIP aquaporin gene expression in seedlings of cucumber cultivars subjected to salinity stress
Authors: F. Yan, D. Qu, Y.Y. Zhao, X.H. Hu, Z.Y. Zhao, Y. Zhang and Z.R. Zou
Aquaporins play a direct role in plant water relation under salt stress, but the effects of 5-aminolevulinic acid (ALA) on aquaporin gene expression in salt-treated plants remain unknown. This study investigated the potential effects of exogenous ALA (50 mg/dm3) on aquaporin expression levels under salt stress (75 mM NaCl) in .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.22.12
- DOI:
- 10.4238/2014.January.22.12
Comparative analysis of polygalacturonase in the fruit of strawberry cultivars
Authors: H.C. Zhou, G. Li, X. Zhao and L.J. Li
The role of polygalacturonase (PG) in the development, ripening, and softening of fruit from two strawberry cultivars with different flesh firmness and softening characteristics was compared. Changes in PG activity and gene expression during development, ripening and softening were measured. The PG genes from each cultivar wer.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.21
- DOI:
- 10.4238/2015.October.19.21