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Gene Expression

Plant Genetics   Research Article

Digital expression analysis of the genes associated with salinity resistance after overexpression of a stress-responsive small GTP-binding RabG protein in peanut

Authors: J.M. Sui, G. Li, G.X. Chen, M.Y. Yu, S.T. Ding, J.S. Wang and L.X. Qiao

The Rab protein family is the largest family of the small GTP-binding proteins. Among them, the RabG genes are known to be responsive to abiotic stresses, but the molecular mechanisms of the stress responses mediated by RabG genes in plants is poorly understood. To investigate the molecular mechanism of AhRabG gene in peanut, .. Read More»

Genet. Mol. Res. 16(1):
gmr16019432
DOI:
10.4238/gmr16019432
Plant Genetics   Research Article

Expression of metallothionein type 2 and 3 genes in Prosopis glandulosa leaves treated with copper

Authors: C. Michel-Lopez, O. Zapata-P�©rez, D. Gonz�¡lez-Mendoza,O. Grimaldo-Juarez, C. Cece�±a-Duran, O. Tzintzun-Camacho

For a better understanding of the strategies that are used by Prosopis glandulosa in heavy metal tolerance, the present study evaluated the gene expression of three metallothioneins (MTs; PgMt2-1, PgMt2, and PgMt3) in plants exposed to sub-lethal concentrations of copper. The PgMt2-1, PgMt2, and PgMt3 sequences were homologous.. Read More»

Genet. Mol. Res. 16(1):
gmr16019490
DOI:
10.4238/gmr16019490
Human Genetics   Research Article

Gene expressions levels of 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPase�² in gill tissue of Mugil cephalus acclimated to low salinity

Authors: L. Li, M. Jiang and X.Q. Shen

Fishes adapt to salinity changes primarily through osmotic pressure regulation, a process often associated with several genes, including 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ. The present study investigated the differential expression of genes 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in the gill tissue of Mu.. Read More»

Genet. Mol. Res. 16(1):
gmr16019444
DOI:
10.4238/gmr16019444
Plant Genetics   Research Article

Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.

Authors: S.M.B. Ca�§�£o T.F. Leite I.G.F. Budzinski T.B. dos Santos M.B.S. Scholz V. Carpentieri-Pipolo D.S. Domingues L.G.E. Vieira L.F.P. Pereira

Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to c.. Read More»

Genet. Mol. Res. 11(3):
2012.September.3.7
DOI:
10.4238/2012.September.3.7
Human Genetics   Research Article

Dithranol downregulates expression of Id1 mRNA in human keratinocytes in vitro

Authors: C. Ronpirin T. Tencomnao

The precise causes of psoriasis, a chronic skin disorder characterized by hyperproliferation of keratinocytes and incomplete keratinization, are unclear. It is known that expression of helix-loop-helix transcription factor Id1, which functions as an inhibitor of differentiation, is upregulated in psoriatic skin. We investigated the effect of the antipsori.. Read More»

Genet. Mol. Res. 11(3):
2012.September.12.12
DOI:
10.4238/2012.September.12.12
Medical Genetics   Research Article

Expression of PCA3 and PSA genes as a biomarker for differential diagnosis of nodular hyperplasia and prostate cancer

Authors: F. Fonseca Coelho, F. Loli Guimar�£es, W.L. Ribeiro Cabral, P.G. Oliveira Salles, E. Cueva Mateo, L. Mendes Nogueira e Nogueira, C.E. Corradi Fonseca and K. Braga Gomes

We evaluated the expression of the PCA3 gene in urine from patients with nodular hyperplasia/benign prostatic hyperplasia (PNH) or adenocarcinoma type prostate cancer (PCa).The study included 59 men: 22 with PCa, 26 with PNH, and 11 with no alterations (controls). Patients’ urine was collected following prostatic massage and quantified by quantitati.. Read More»

Genet. Mol. Res. 14(4):
2015.October.28.13
DOI:
10.4238/2015.October.28.13
Plant Genetics   Research Article

Effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus

Authors: Q.D. Jiang1,2 and X.P. Li1

The hypothalamus is an important component of the nervous system, and neuropeptide Y (NPY), proopiomelanocortin (POMC), and neuromedin U (NMU) are key players in physiological regulation. Puerarin is important for nerve regulation. We investigated the effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus. The results showed .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017362
DOI:
10.4238/gmr.15017362
Plant Genetics   Research Article

Efficient isolation of high-quality RNA from lotus Nelumbo nucifera ssp nucifera tissues

Authors: Y.J. Zhang X.Y. Hao Z.S. Liang W.D. Ke H.B. Guo

Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-qua.. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.14
DOI:
10.4238/2013.January.24.14
Human Genetics   Research Article

Comparative study of 13 candidate genes applying multi-reference normalization to detect the expression of different fineness in skin tissues of wool sheep

Authors: Y.Z. Tian, T. Usman, K.C. Tian, J. Di, X.X. Huang, X.M. Xu, H. Tulafu, W.W. Wu, X.F. Fu, Y. Bai, M. Tuerxun and A. Maimaiti

Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provid.. Read More»

Genet. Mol. Res. 16(1):
gmr16018905
DOI:
10.4238/gmr16018905
Human Genetics   Research Article

CsSAD: a fatty acid desaturase gene involved in abiotic resistance in Camellia sinensis (L.)

Authors: Z.T. Ding1,2*, J.Z. Shen1,2*, L.L. Pan1,2, Y.U. Wang1,2, Y.S. Li3, Y. Wang4 andH.W. Sun5

Tea (Camellia sinensis L.) is a thermophilic evergreen woody plant that has poor cold tolerance. The SAD gene plays a key role in regulating fatty acid synthesis and membrane lipid fluidity in response to temperature change. In this study, full-length SAD cDNA was cloned from tea leaves using rapid amplification of cDNA ends and polymerase chain reaction .. Read More»

Genet. Mol. Res. 15(1):
gmr.15017512
DOI:
10.4238/gmr.15017512