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Gene Expression

Human Genetics   Research Article

Identification and characterization of TGF?²-dependent and -independent cis-regulatory modules in the C4ST-1/CHST11 locus

Authors: C.M. Willis, J.L. Wrana and M. Kl?¼ppel

Chondroitin-4-sulfotransferase-1(C4ST-1)/carbohydrate sul­fotransferase 11 (CHST11) is a Golgi-bound enzyme involved in the biosyn­thesis of the glycosaminoglycan chondroitin sulfate. The sulfation pattern of chondroitin is tightly regulated during development, injury and disease, with the temporal and spatial expressi.. Read More»

Genet. Mol. Res. 8(4):
vol8-4gmr673
DOI:
10.4238/vol8-4gmr673
Human Genetics   Research Article

Correlation-based linear discriminant classification for gene expression data

Authors: M. Pan and J. Zhang

Microarray gene expression technology provides a systematic approach to patient classification. However, microarray data pose a great computational challenge owing to their large dimensionality, small sample sizes, and potential correlations among genes. A recent study has shown that gene-gene correlations have a positive effe.. Read More»

Genet. Mol. Res. 16(1):
gmr16019357
DOI:
10.4238/gmr16019357
Animal Genetics   Research Article

Involvement of ERK1/2 signaling in proliferation of eight liver cell types during hepatic regeneration in rats

Authors: X.G. Chen C.S. Xu Y.M. Liu

It has been well established that ERK1/2 signaling, often subdivided into nine types of pathways, can regulate the hepatocyte proliferative response during liver regeneration. However, the effect of ERK1/2 signaling on the proliferation of other hepatic cell types remains unclear. We isolated and purified 8 liver cell types at 10 time points after 2/3 hep.. Read More»

Genet. Mol. Res. 12(1):
2013.March.11.14
DOI:
10.4238/2013.March.11.14
Plant Genetics   Research Article

Cloning and characterization of SPL-family genes in the peanut (Arachis hypogaea L.)

Authors: M. Li1*, S.Z. Zhao1*, C.Z. Zhao1, Y. Zhang1, H. Xia1, J. Lopez-Baltazar2, S.B. Wan1 and X.J. Wang1

SQUAMOSA promoter-binding protein-like (SPL) proteins play crucial roles in plant growth, development, and responses to environmental stressors. The peanut (Arachis hypogaea L.) is a globally important oil crop. In this study, we cloned the full-length cDNA of 15 SPLs in the peanut by transcriptome sequencing and rapid amplification of cDNA ends, and anal.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017344
DOI:
10.4238/gmr.15017344
Plant Genetics   Research Article

Cloning and characterization of defense-related genes from Populus szechuanica infected with rust fungus Melampsora larici-populina

Authors: Z.J. Chen1,2, Z.M. Cao1, Z.D. Yu1 and D. Yu1

Characterization of defense-related genes is critical for breeding disease-resistant poplar varieties and for better management and control of leaf rust disease. In the present study, full-length cDNAs of five Populus szechuanica defense-related (PsDR) genes, pathogen-related protein 1 (PsPR1), β-1,3-glucanase (PsGns), thaumatin-like protein 1 (PsTLP.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017314
DOI:
10.4238/gmr.15017314
Animal Genetics   Research Article

Differential expression of the lethal gene Luteus-Pa in cacao of the Parinari series

Authors: B.C. Rehem1, A.-A.F. Almeida1, G.S.F. Figueiredo1, A.S. Gesteira2, S.C. Santos1, R.X. Corr?ªa1, M.M. Yamada3 and R.R. Valle3

The recessive lethal character Luteus-Pa is found in cacao (Theobroma cacao) genotypes of the Parinari series (Pa) and is characterized by expression of leaf chlorosis and seedling death. Several genotypes of the Pa series are bearers of the gene responsible for the expression of the Luteus-Pa character, which can be used as a tool for determining relatio.. Read More»

Genet. Mol. Res. 15(1):
gmr.15014925
DOI:
10.4238/gmr.15014925
Bioinformatics   Research Article

Optimal clone identifier for genomic shotgun libraries: â??OC Identifier toolâ?

Authors: Cantao ME, Ferreira JE and Lemos EGM

In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of puttin.. Read More»

Genet. Mol. Res. 6(4):
Microbial Genetics   Research Article

Genome-wide partial correlation analysis of Escherichia coli microarray data

Authors: Veiga DFT, Vicente FFR, Grivet M, de la Fuente A and Vasconcelos ATR

Transcriptional control is an essential regulatory mechanism employed by bacteria. Much about transcriptional regulation remains to be discovered, even for the most widely studied bacterium, Escherichia coli. In the present study, we made a genome-wide loworder partial correlation analysis of E. coli microarray data with the p.. Read More»

Genet. Mol. Res. 6(4):
Animal Genetics   Research Article

mRNA and protein expression levels of four candidate genes for ear size in Erhualian and Large White pigs

Authors: L.C. Zhang, J. Liang, L. Pu, Y.B. Zhang, L.G. Wang, X. Liu, H. Yan, L.X. Wang

Porcine ear size is an important characteristic for distinguishing among pig breeds. In a previous genome-wide association study of porcine ear size, LEM domain-containing 3 (LEMD3), methionine sulfoxide reductase B3 (MSRB3), high mobility group AT-hook 2 (HMGA2), and Wnt inhibitory factor 1 (WIF1) were implicated as important.. Read More»

Genet. Mol. Res. 16(2):
gmr16029252
DOI:
10.4238/gmr16029252
Plant Genetics   Research Article

Identification of warm day and cool night conditions induced flowering-related genes in a Phalaenopsis orchid hybrid by suppression subtractive hybridization

Authors: D.M. Li, F.B. L??, G.F. Zhu, Y.B. Sun, Y.C. Xu, M.D. Jiang, J.W. Liu and Z. Wang

The influence of warm day and cool night conditions on induction of spikes in Phalaenopsis orchids has been studied with respect to photosynthetic efficiency, metabolic cycles and physiology. However, molecular events involved in spike emergence induced by warm day and cool night conditions are not clearly understood... Read More»

Genet. Mol. Res. 13(3):
2014.February.14.7
DOI:
10.4238/2014.February.14.7