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Gene

Plant Genetics   Research Article

Variability among Capsicum baccatum accessions from Goi�¡s, Brazil, assessed by morphological traits and molecular markers

Authors: A.L.A. Martinez, J.S.P. Ara�ºjo, C.F. Ragassi, G.S.C. Buso and F.J.B. Reifschneider

Capsicum peppers are native to the Americas, with Brazil being a significant diversity center. Capsicum baccatum accessions at Instituto Federal (IF) Goiano represent a portion of the species genetic resources from central Brazil. We aimed to characterize a C. baccatum working collection comprising 27 accessions and 3 commerci.. Read More»

Genet. Mol. Res. 16(3):
gmr16039074
DOI:
10.4238/gmr16039074
Plant Genetics   Research Article

An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae)

Authors: S.R. Arruda, D.G. Pereira, M.M. Silva-Castro, M.G. Brito and A.M. Waldschmidt

Some species are characterized by a high content of tannins, alkaloids, and phenols in their leaves. These secondary metabolites are released during DNA extraction and might hinder molecular studies based on PCR (polymerase chain reaction). To provide an efficient method to extract DNA, Mimosa tenuiflora, an important legumino.. Read More»

Genet. Mol. Res. 16(3):
gmr16039063
DOI:
10.4238/gmr16039063
Plant Genetics   Research Article

Identification of Colletotrichum isolates from Capsicum chinense in Amazon

Authors: C.V.S. de Oliveira, K.S. Matos, D.M.C. de Albuquerque, R.E. Hanada and G.F. da Silva

Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colle.. Read More»

Genet. Mol. Res. 16(2):
gmr16029601
DOI:
10.4238/gmr16029601
Microbial Genetics   Research Article

Generic relationships among Molluginaceae inferred from a molecular phylogenetic analysis of the matK gene

Authors: M.A. Ali, J. Lee and F. Al-Hemaid

The family Molluginaceae (order Caryophyllales) is considered polyphyletic based on the photosynthetic pathway, C4 evolution, and phylogeny of the family. This inference was made based on photosynthetic, anatomical, and molecular datasets. The generic circumscription of this family has greatly been changed owing to the placeme.. Read More»

Genet. Mol. Res. 16(2):
gmr16029295
DOI:
10.4238/gmr16029295
Plant Genetics   Research Article

Genetic variability among elite popcorn lines based on molecular and morphoagronomic characteristics

Authors: J.F. dos Santos, C.A. Mangolin, M.F.P.S. Machado, C.A. Scapim, W. Giordani and L.S.A. Gon�§alves

Knowledge of genetic diversity among genotypes and relationships among elite lines is of great importance for the development of breeding programs. Therefore, the objective of this study was to evaluate genetic variability based on the morphoagronomic and molecular characterization of 18 elite popcorn (Zea mays var. everta) li.. Read More»

Genet. Mol. Res. 16(2):
gmr16029243
DOI:
10.4238/gmr16029243
Animal Genetics   Research Article

Genetic correlation and genome-wide association study (GWAS) of the length of productive life, days open, and 305-days milk yield in crossbred Holstein dairy cattle

Authors: P. Saowaphak, M. Duangjinda, S. Plaengkaeo, R. Suwannasing and W. Boonkum

In this study, we estimated the genetic parameters and identified the putative quantitative trait loci (QTL) associated with the length of productive life (LPL), days open (DO), and 305-day milk yield for the first lactation (FM305) of crossbred Holstein dairy cattle. Data comprising 4,739 records collected between 1986 and 20.. Read More»

Genet. Mol. Res. 16(2):
gmr16029091
DOI:
10.4238/gmr16029091
Microbial Genetics   Research Article

Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis

Authors: A.R.E.O. Xavier, A.C. Almeida, C.N. Souza, L.M.V Silva, A.X.A. Ruas, D.A. Sanglard, A.F.M. J�ºnior , A.M.E. Oliveira and M.A.S. Xavier

The Staphylococcus aureus is the most common isolated microorganism in ruminant animal species diagnostic with clinical or subclinical mastitis. Dairy herds with these diseases can transfer S. aureus into the milk supply, which can lead to food poisoning in humans. The objective of this study was to evaluate the profile of ant.. Read More»

Genet. Mol. Res. 16(2):
gmr16029709
DOI:
10.4238/gmr16029709
Plant Genetics   Research Article

Transferability of microsatellite markers in Syagrus coronata (Mart.) Becc. (Arecaceae), an iconic palm tree from the Brazilian semiarid region

Authors: R.R. Simplicio, D.G. Pereira and A.M. Waldschmidt

The licuri palm Syagrus coronata plays a key role in the ecology and economy of Brazilian semiarid region. Nonetheless, genetic data about populations of this species are absent even though the intensive and uncontrolled exploitation since colonial periods has threatened the sustainability and viability of licuri populations. .. Read More»

Genet. Mol. Res. 16(2):
gmr16029699
DOI:
10.4238/gmr16029699
Plant Genetics   Research Article

Selection of candidate reference genes and validation for real-time PCR studies in rice plants exposed to low temperatures

Authors: P.A. Auler, L.C. Benitez, M.N. do Amaral, I.L. Vighi, G.S. Rodrigues, L.C. da Maia and E.J.B. Braga

Rice is a cereal that presents a great ability to adapt to different soil and climate conditions. However, as it is a tropical crop with C3 metabolism, it performs better in warm temperatures with high solar radiation. Tolerance to stress caused by low temperatures is a highly complex process that involves various metabolic pa.. Read More»

Genet. Mol. Res. 16(2):
gmr16029695
DOI:
10.4238/gmr16029695
Plant Genetics   Research Article

Genome-wide analysis of salinity-stress induced DNA methylation alterations in cotton (Gossypium hirsutum L.) using the Me-DIP sequencing technology

Authors: X.K. Lu, N. Shu, J.J. Wang, X.G. Chen, D.L. Wang, S. Wang, W.L. Fan, X.N. Guo, L.X. Guo and W.W. Ye

Cytosine DNA methylation is a significant form of DNA modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. Although the reference genomes of cotton (Gossypium hirsutum L.) have been publically available, the salinity-stress-induced DNA methylome alterations in cotton are not well unde.. Read More»

Genet. Mol. Res. 16(2):
gmr16029673
DOI:
10.4238/gmr16029673