Gene
Effect of RNAi-mediated silencing of Livin gene on biological properties of colon cancer cell line LoVo
Authors: A.M. Zou, H.F. Wang, W.F. Zhu, F.X. Wang and J.J. Shen
This study aimed to investigate the effect of RNAi-mediated silencing of the Livin gene on biological properties of the colon cancer cell line LoVo. Interference vectors pSilencer4.1-Ll and pSilencer4.1-L2 targeting the Livin gene were constructed and transfected into LoVo cells. The expression of the Livin gene was determined.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.8
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.8
Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes
Authors: X.J. Rong, Y.J. Xu, Q.Y. Wang, M.J. Liao, X.Z. Liu, C.Y. Pan,Z. Zhang, Y.G. Wang
Coilia ectenes (Jordan and Seale 1905) is an important anadromous species that is an important resource at risk of extinction because of over-fishing, pollution, and coastal construction. To evaluate the genetic diversity of C. ectenes for use in breeding programs, elite microsatellite-enriched libraries were constructed and n.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.11
- DOI:
- 10.4238/2013.November.26.11
Molecular cloning and expression of the porcine S14R gene in Escherichia coli
Authors: Y.J. Guo1*, G.Z. Liu1*, C.M. Wang1, Y.Y. Wang2, H.J. Li2, K. Zhong2, W.F. Lu2, Y.L. Wang2, G.Y. Yang2
We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragm.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.6
- DOI:
- 10.4238/2013.October.10.6
Association of SIRT2 gene polymorphisms with body measurement and growth traits of Qinchuan cattle
Authors: L.S. Gui, W.C. Yang, C.P. Zhao, S.J. Wei, Z.D. Zhao and L.S. Zan
Silent information regulator 2 (SIRT2), a member of the Sirtuin family of class III nicotinamide adenine dinucleotide-dependent protein deacetylases, plays an important role in senescence, metabolism, and apoptosis. This study was conducted to detect potential polymorphisms of the bovine SIRT2 gene and explore their relationships with meat quality and bod.. Read More»
- Genet. Mol. Res. 13(4):
Developmental changes in IGF-I and MyoG gene expression and their association with meat traits in sheep
Authors: W. Sun, R. Su1, D. Li, H.H. Musa, Y. Kong, J.T. Ding, Y.H. Ma, L. Chen, Y.F. Zhang and W.Z. Wu
In the present study, real time-polymerase chain reaction was applied to analyze the expression of IGF-I and MyoG genes in Hu sheep longissimus dorsi at different growth stages and their association with meat traits. Expression of the IGF-I gene in Hu sheep differed significantly between males and females at the two day-old (0.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.6
- DOI:
- 10.4238/2014.April.14.6
Inbreeding effects in Solanum lycocarpum A. St.-Hil populations, an endangered species of the Brazilian Cerrado
Authors: T.M. Moura, M.V.B.M. Siqueira, G.C.X. Oliveira
The inbreeding effective population size is an estimate of inbreeding and genetic drift in populations. It is an important tool for conservation genetics because it represents the number of individuals that are effectively contributing alleles to the subsequent generations. Several studies have been published in the last decad.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.10
- DOI:
- 10.4238/2013.November.26.10
Expression and enzymatic activity of phenylalanine ammonia-lyase and p-coumarate 3-hydroxylase in mango (Mangifera indica �Ataulfo�) during ripening
Authors: H. Palafox-Carlos, C.A. Contreras-Vergara, A. Muhlia-Almaz�¡n, M.A. Islas-Osuna and G.A. Gonz�¡lez-Aguilar
Phenylalanine ammonia lyase (PAL) and p-coumarate 3-hydroxylase (C3H) are key enzymes in the phenylpropanoid pathway. The relative expression of PAL and C3H was evaluated in mango fruit cultivar ‘Ataulfo’ in four ripening stages (RS1, RS2, RS3, and RS4) by quantitative polymerase chain reaction. In addition, enzyme.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.10
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.10
Estimates of genetic parameters for total milk yield over multiple ages in Brazilian Murrah buffaloes using different models
Authors: R.C. Sesana, F. Baldi, R.R.A. Borquis, A.B. Bignardi, N.A. Hurtado-Lugo, L. El Faro, L.G. Albuquerque and H. Tonhati
The objective of this study was to estimate variance components and genetic parameters for accumulated 305-day milk yield (MY305) over multiple ages, from 24 to 120 months of age, applying random regression (RRM), repeatability (REP) and multi-trait (MT) models. A total of 4472 lactation records from 1882 buffaloes of the Murr.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.7
- DOI:
- 10.4238/2014.April.14.7
Critical evaluation of transcription factor Atf2 as a candidate modulator of alcohol preference in mouse and human populations
Authors: L.S. Wang, Y. Jiao, Y. Huang, X.Y. Liu, G. Gibson, B. Bennett,K.M. Hamre, D.W. Li, H.Y. Zhao, J. Gelernter, H.R. Kranzler,L.A. Farrer, L. Lu, Y.J. Wang, W.K. Gu
In prior work, congenic strains carrying the DBA/2Igb (D2) region of chromosome 2 (Chr2) for alcohol preference were bred onto a C57BL/6Ibg (B6) background and as predicted were found to reduce voluntary consumption. Subsequently, interval-specific congenic recombinant strains (ISCRS) were generated and also tested. These ISCR.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.9
- DOI:
- 10.4238/2013.November.26.9
Association of the estrogen receptor-�² gene RsaI and AluI polymorphisms with human idiopathic thin endometrium
Authors: A.W. Le, Z.H. Wang, R. Yuan, L.L. Shan, T.H. Xiao, R. Zhuo, Y. Shen
The aim of this study was to investigate the potential correlation between polymorphisms of the estrogen receptor (ER)-β gene (RsaI and AluI) and ethnic Han Chinese human idiopathic thin endometrium. A total of 120 patients with idiopathic thin endometrium and 120 sterility patients with normal endometrium thickness (cont.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.26.7
- DOI:
- 10.4238/2013.November.26.7