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Gene

Plant Genetics   Research Article

Genetic diversity analysis in Tunisian perennial ryegrass germplasm as estimated by RAPD, ISSR, and morpho-agronomical markers

Authors: S. Ghariani, H. Elazreg, N. Chtourou-Ghorbel, M. Chakroun and N. Trifi-Farah

Tunisia is rich in diverse forage and pasture species including perennial ryegrass. In order to enhance forage production and improve agronomic performance of this local germplasm, a molecular analysis was undertaken. Random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and morpho-agronomical traits markers were used for genetic d.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.40
DOI:
10.4238/2015.December.23.40
Microbial Genetics   Research Article

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Authors: W.Y. Xuan, Y. Zhang, Z.Q. Liu, D. Feng and M.Y. Luo

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. m.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.27
DOI:
10.4238/2015.August.19.27
Medical Genetics   Research Article

Polymorphic microsatellite markers in the traditional Chinese medicinal plant Paris polyphylla var. yunnanensis

Authors: Y. Song, M.F. Li, J. Xu, Z. Zha and N.Z. Chen

Paris polyphylla var. yunnanensis is a traditional Chinese medicinal plant and is listed as vulnerable by the IUCN. This medicinal herb is well known for its analgesic and anti-inflammatory properties, most notably as an ingredient of the hemostatic compound “Yunnan Baiyao”. However, over-exploitation of the plant for economic purposes is push.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.29
DOI:
10.4238/2015.August.19.29
Medical Genetics   Research Article

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

Authors: M.P. Liu, H.Y. Du, G.P. Zhu, D.L. Fu and W.Y. Tana

Simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) markers were used to evaluate genetic diversity among 22 sweet kernel apricot accessions and 12 cultivars in China to provide information on how to improve the utilization of kernel apricot germplasms. The results showed that 10 pairs of SSR primers screened from 40 primer pairs amplifie.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.4
DOI:
10.4238/2015.August.19.4
Human Genetics   Research Article

Genetic diversity of popcorn genotypes using molecular analysis

Authors: F.S. Resh, C.A. Scapim, C.A. Mangolin, M.F.P.S. Machado, A.T. do Amaral Jr., H.C.C. Ramos and M. Vivas

In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were mon.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.16
DOI:
10.4238/2015.August.19.16
Microbial Genetics   Research Article

Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge)

Authors: C. Cheng, J. Hu, Y. Zhi, J.J. Su, X.K. Zhang and B.Q. Huang

ADP-glucose pyrophosphorylase (ADPGlcPPase) controls the first committed step of starch synthesis by catalyzing the biosynthesis of ADP-glucose from glucose-phosphate and ATP. It is a tetrameric protein consisting of two small and two large subunits. The small subunits have a catalytic function, while the large subunits regulate the enzyme activity. Cyper.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.18
DOI:
10.4238/2015.December.23.18
Human Genetics   Research Article

Topological centrality-based identification of hub genes and pathways associated with acute viral respiratory infection in infants

Authors: X.Y. Liu, G.Q. Li, Y. Ma and L.J. Zhao

The objective of this study was to identify hub genes and pathways associated with acute respiratory infection (ARI) in infants based on gene expression profiles. Differentially expressed genes (DEGs) between ARI and normal (controls) infants were identified based on linear modeling of the microarray data using Limma package. A protein-protein interaction.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.21
DOI:
10.4238/2015.December.23.21
Microbial Genetics   Research Article

DNA barcoding and phylogenetic relationships of genera Picoides and Dendrocopos (Aves: Picidae)

Authors: Z.H. Huang, F.Y. Tu and X.J. Liao

Picoides and Dendrocopos are two closely related genera of woodpeckers (family Picidae), and members of these genera have long been the subjects of phylogenetic debate. Mitochondrial cytochrome c oxidase subunit I (COI) is a powerful marker for the identification and phylogenetic study of animal species. In the present study, we analyzed the COI barcodes .. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.24
DOI:
10.4238/2015.December.23.24
Animal Genetics   Research Article

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

Authors: S.B. Jin, H.T. Fu, S.F. Jiang, Y.W. Xiong, H. Qiao, W.Y. Zhang, Y.S. Gong and Y. Wu

The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. Howeve.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.27
DOI:
10.4238/2015.December.23.27
Microbial Genetics   Research Article

Can Spirulina maxima reduce the mutagenic potential of sibutramine?

Authors: R.P. Araldi, N.P. Santos, T.B. Mendes, L.B. Carvalho, E.T. Ito, P.L. de-S�¡-J�ºnior and EB Souza

The worldwide obesity pandemic requires the use of anti-obesity drugs. Sibutramine is an anti-obesity drug that has been used worldwide but is indiscriminately consumed in Brazil. Several studies have demonstrated that sibutramine promotes weight loss and weight maintenance, but several side effects have been associated with its systematic consumption. Fo.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.33
DOI:
10.4238/2015.December.23.33