Gene
Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)
Authors: Z.Z. Xiao, W.W. Chen, W. Bao, R. Wang and Y.Y. Li
Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populatio.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028322
- DOI:
- 10.4238/gmr.15028322
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
Authors: M.S.P. De Azevedo, C. Santos Rocha, V.B. Pereira, A.F. De Oliveira Junior, C.S. De Sousa, V. Azevedo, J.G. LeBlanc, J.M. Chatel and A. Miyoshi
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with hig.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.36
- DOI:
- 10.4238/2015.December.23.36
HVSI polymorphism indicates multiple origins of mtDNA in the Hazarewal population of Northern Pakistan
Authors: N. Akbar, H. Ahmad, M.S. Nadeem, B.E. Hemphill, K. Muhammad, W. Ahmad and M. Ilyas
Mitochondrial DNA (mtDNA) is an important tool used to explore ethnogenetics and the evolutionary history of human populations. In this study, hypervariable segment I (HVSI) from mtDNA was analyzed to establish the genetic lineage of the Hazarewal populations residing in the Mansehra and Abbottabad districts of Northern Pakist.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027167
- DOI:
- 10.4238/gmr.15027167
Association between ARNTL (BMAL1) rs2278749 polymorphism T >C and susceptibility to Alzheimer disease in a Chinese population
Authors: Q. Chen, X.D. Peng, C.Q. Huang, X.Y. Hu and X.M. Zhang
In the present study, we examined whether the ARNTL (BMAL1) rs2278749 T/C polymorphism was associated with the susceptibility to Alzheimer disease (AD). This case-control study examined the genotypes of apolipoprotein E (APOE e4) and BMAL1 rs2278749 T/C using restriction fragment length polymorphism and the TaqMan assay, respectively. A total of 296 unrel.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.39
- DOI:
- 10.4238/2015.December.23.39
Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids
Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu
Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»
- Genet. Mol. Res. 15(2):
Detection of a mutation at codon 43 of the rpsL gene in Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae by PCR-RFLP
Authors: Y. Zhang, X. Yang, F.Y. Zhou, A.F. Zhang, X.F. Zhu, Y. Chen, M.G. Zhou and T.C. Gao
The aim of this study was to develop a method to detect a point mutation in the ribosomal S12 protein (rpsL) gene in streptomycin-resistant strains of Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was developed to detect a point mutation in codon 43 of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.6
- DOI:
- 10.4238/2015.December.28.6
Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paran�¡ River basin): A case study
Authors: W. Frantine-Silva, D.G. Ferreira, R.H.C. Nascimento, J.F. Fracasso, J.E. Conte, F.P. Ramos, S. Carvalho and B.A. Galindo
Most studies of diversity and genetic structure in neotropical fish have focused on commercial species from large rivers or their reservoirs. However, smaller tributaries have been identified as an important alternative migratory route, with independent pools of genetic diversity. In this context, the present study aimed to evaluate genetic diversity and .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.13
- DOI:
- 10.4238/2015.December.28.13
Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin
Authors: S.O. Bafeel
The genetic diversity of Saudi locally growing sorghum (Sorghum bicolor) cultivars has not been thoroughly characterized. To understand the genomic patterns of diversification in Saudi sorghum cultivars (N = 7), random amplified polymorphic DNA (RAPD) was used as a rapid, inexpensive method for providing information regarding genomic variability below the.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.3
- DOI:
- 10.4238/2015.August.21.3
Association of the PPAR�³2 Pro12Ala polymorphism with increased risk of cardiovascular diseases
Authors: Y. Li, J. Zhu and J.Q. Ding
This meta-analysis investigated the correlation between the PPARγ2 Pro12Ala polymorphism and cardiovascular disease (CVD). Electronic database and manual searches were conducted to retrieve studies published relevant to the PPARγ2 Pro12Ala polymorphism and CVD. Rigorous inclusion and exclusion criteria were employed for selection of high-quali.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.15
- DOI:
- 10.4238/2015.December.28.15
PI3K-AKT pathway polymerase chain reaction (PCR) array analysis of epilepsy induced by type II focal cortical dysplasia
Authors: Y.X. Lin, K. Lin, X.X. Liu, D.Z. Kang, Z.X. Ye, X.F. Wang, S.F. Zheng, L.H. Yu and Z.Y. Lin
The aims of this study were to observe the differential expression of PI3K-AKT pathway-related genes in seizure-inducing brain lesions in type II focal cortical dysplasia, and to explore the relationship between gene expression and histological changes in dysplastic foci and their epileptogenic mechanism. Typical lesions in brain tissue from three patient.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.5
- DOI:
- 10.4238/2015.August.21.5