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Gene

Animal Genetics   Research Article

Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense

Authors: W.Y. Zhang, S.F. Jiang, Y.W. Xiong, H.T. Fu1,, H. Qiao, S.M. Sun, Y.S. Gong and S.B. Jin

The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its e.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.30.4
DOI:
http://dx.doi.org/10.4238/2015.April.30.4
Microbial Genetics   Research Article

Analysis of drug-resistant gene detection of blaOXA-like genes from Acinetobacter baumannii

Authors: D.K. Yang, H.J. Liang, H.L. Gao, X.W. Wang and Y. Wang

Our study determines the resistance gene profile of a set of Acinetobacter baumannii hospital isolates. A. baumannii is responsible for nosocomial outbreaks and sporadic infections. We extracted and PCR amplified bacterial DNA isolated from patients with ages below 60 years (23.36%) and above 60 years (76.64%). Most of the patients were admitted in the IC.. Read More»

Genet. Mol. Res. 14(4):
2015.December.29.7
DOI:
10.4238/2015.December.29.7
Plant Genetics   Research Article

Assessment of genetic diversity of cucumber cultivars in China based on simple sequence repeats and fruit traits

Authors: Y.T. Yang, Y. Liu, F. Qi, L.L. Xu, X.Z. Li, L.J. Cong, X. Guo, S.X. Chen and Y.L. Fang

The cucumber (Cucumis sativus L.) is an important crop grown worldwide. In this study, the genetic diversity of 42 cucumber cultivars in China was analyzed using 51 pairs of simple sequence repeat (SSR) primers. These primers identified 129 polymorphic loci, 95.6% of which were polymorphic. The mean effective number of alleles, mean Nei’s gene diver.. Read More»

Genet. Mol. Res. 14(4):
2015.December.29.10
DOI:
10.4238/2015.December.29.10
Human Genetics   Research Article

Association of SNPs in the PPAR�³ gene and hypertension in a Mongolian population

Authors: L. Yang, R.G. Tian, P.Y. Chang, M.R. Yan and X.L. Su

The association of single nucleotide polymorphisms (SNPs) in PPARγ with hypertension is controversial. The aim of the present study was to clarify the contributions of PPARγ genetic variants to hypertension through an association study. A total of 414 unrelated Mongolian herdsmen and 524 Han farmers were included in this study. Fourteen intron.. Read More»

Genet. Mol. Res. 14(4):
2015.December.29.39
DOI:
10.4238/2015.December.29.39
Animal Genetics   Research Article

MicroRNA screening and functional study of obliterative bronchiolitis in a rat model simulating lung transplantation

Authors: J. Wang, H. Cao, X. Hong, G.H. Chen, H.M. Fan, Q.C. Li, Z.M. Liu and K.F. Li

The aim of this study was to provide the experimental basis for effective prevention and treatment of obliterative bronchiolitis (OB) by studying the changes on the microRNA (miRNA) expression profile after an orthotopic tracheal transplantation (OTT) simulating lung transplantation (LT). The OTT was performed on inbred rats to establish an OB animal mode.. Read More»

Genet. Mol. Res. 14(4):
2015.December.29.40
DOI:
10.4238/2015.December.29.40
Microbial Genetics   Research Article

Direct preparation protocol to obtain mitotic chromosomes from canine mammary tumors

Authors: C.S.D. Morais, P.R.A.M. Affonso, J.A. Bitencourt and A.A. Wenceslau

Currently, mammary neoplasms in female canines are a serious problem in veterinary clinics. In addition, the canine species is an excellent disease model for human oncology because of the biological and genetic similarities between the species. Cytogenetics has allowed further study of the characterization of neoplasms in canines. We hypothesized that the.. Read More»

Genet. Mol. Res. 14(4):
2015.December.29.49
DOI:
10.4238/2015.December.29.49
Animal Genetics   Research Article

SNPs in the 5'-regulatory region of the tyrosinase gene do not affect plumage color in ducks (Anas platyrhynchos)

Authors: N.N. Zhang, J.W. Hu, H.H. Liu, H.Y. Xu, H. He and L. Li

Tyrosinase, encoded by the TYR gene, is the rate-limiting enzyme in the production of melanin pigment. In this study, plumage color separation was observed in Cherry Valley duck line D and F1 and F2 hybrid generations of Liancheng white ducks. Gene sequencing and bioinformatic analysis were applied to the 5'-regulatory region of TYR, to explore the connec.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.11
DOI:
10.4238/2015.December.28.11
Animal Genetics   Research Article

Construction and characterization of recombinant adenovirus carrying a mouse TIGIT-GFP gene

Authors: J.M. Zheng, J.L. Cui, W.T. He, D.W. Yu, Y. Gao, L. Wang, Z.K. Chen and H.M. Zhou

Recombinant adenovirus vector systems have been used extensively in protein research and gene therapy. However, the construction and characterization of recombinant adenovirus is a tedious and time-consuming process. TIGIT is a recently discovered immunosuppressive molecule that plays an important role in maintaining immunological balance. The constructio.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.14
DOI:
10.4238/2015.December.28.14
Human Genetics   Research Article

Relationship between rs1047763 polymorphism of the C1GALT1 gene and susceptibility to immunoglobulin A nephropathy in Xinjiang Uyghur people

Authors: J.N. Xue, Y. Guo, X. Song, F. Xue, S.F. Yang, H. Jiang, R.Z.W.A. Bu-La and C. Lu

We explored the relationship between rs1047763, a single-nucleotide polymorphism (SNP) of the C1GALT1 gene, and genetic susceptibility to immunoglobulin A nephropathy (IgAN) in Xinjiang Uyghur people. The study comprised 90 patients with IgAN and 90 normal controls recruited from Uyghur people. The distribution of the rs1047763 polymorphism of C1GALT1 in .. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.17
DOI:
10.4238/2015.December.28.17
Animal Genetics   Research Article

Genetic association of growth traits with carcass and meat traits in Nellore cattle

Authors: R.L. Tonussi, R. Espigolan, D.G.M. Gordo, A.F.B. Magalh�£es, G.C. Venturini, F. Baldi, H.N. de Oliveira, L.A.L. Chardulo, H. Tonhati and L.G. de Albuquerque

The aim of this study was to estimate genetic and phenotypic associations of growth traits with carcass and meat traits in Nellore cattle. Data from male and female animals were used for weaning weight (WW; N = 241,416), yearling weight (YW, N = 126,596), weight gain from weaning to yearling (GWY, N = 78,687), and yearling hip height (YHH, N = 90,720), re.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.20
DOI:
10.4238/2015.December.28.20