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Gene

Microbial Genetics   Research Article

Identification of Chromobacterium violaceum genes with potential biotechnological application in environmental detoxification

Authors: Marta S.P. Carepo, Juliana S. Nina de Azevedo, Jorge I.R. Porto, Alexandra R. Bentes-Sousa, Jacqueline da Silva Batista, Artur L.C. da Silva, Maria P.C. Schneider

Chromobacterium violaceum is a Gram-negative bacterium found in a wide variety of tropical and subtropical ecosystems. The complete genome sequence of C. violaceum ATCC 12472 is now available, and it has considerable biotechnological potential for various applications, such as environmental detoxification, as well as medical a.. Read More»

Genet. Mol. Res. 3(1):
Microbial Genetics   Research Article

Transport genes of Chromobacterium violaceum: an overview

Authors: Thalles Barbosa Grangeiro, Daniel Macedo de Melo Jorge, Walderly Melga�§o Bezerra, Ana Tereza Ribeiro Vasconcelos, Andrew John George Simpson

The complete genome sequence of the free-living bacterium Chromobacterium violaceum has been determined by a consortium of laboratories in Brazil. Almost 500 open reading frames (ORFs) coding for transport-related membrane proteins were identified in C. violaceum, which represents 11% of all genes found. The main class of tran.. Read More»

Genet. Mol. Res. 3(1):
Microbial Genetics   Research Article

Chemotaxis and flagellar genes of Chromobacterium violaceum

Authors: Maristela Pereira, Juliana Alves Parente, Luiz Artur Mendes Bataus, Divina das Dores de Paula Cardoso, Renata Bastos Ascen�§o Soares, C�©lia Maria de Almeida Soares

The availability of the complete genome of the Gram-negative β-proteobacterium Chromobacterium violaceum has increasingly impacted our understanding of this microorganism. This review focuses on the genomic organization and structural analysis of the deduced proteins of the chemosensory adaptation system of C. violaceum. .. Read More»

Genet. Mol. Res. 3(1):
Microbial Genetics   Research Article

Gene expression in Chromobacterium violaceum

Authors: Rosane Silva, J�ºlia R. Araripe, Edson Rondinelli, Tur�¡n P. ��rm�©nyi

The repertoire of 4,431 open reading frames (ORFs), eight rRNA operons and 98 tRNA genes of Chromobacterium violaceum must be expressed in a regulated manner for successful adaptation to a wide variety of environmental conditions. To accomplish this feat, the organism relies on protein machineries involved in transcription, RN.. Read More»

Genet. Mol. Res. 3(1):
Animal Genetics   Research Article

Behavioral genetics of Drosophila ananassae

Authors: Shree Ram Singh, Bashisth N. Singh

Drosophila ananassae has a unique status among Drosophila species because of certain peculiarities in its genetic behavior. The most unusual feature of this species is its relatively high frequency of spontaneous male recombination. The results of studies on non-sexual behavior, such as phototactic responses, eclosion rhythm, .. Read More»

Genet. Mol. Res. 2(4):
Plant Genetics   Research Article

Putative pyrophosphate phosphofructose 1-kinase genes identified in sugar cane may be getting energy from pyrophosphate

Authors: S.J. Suzuki, M.A. Mutton, J.M. Pizauro, M. I.T. Ferro, M. V.F. Lemos, M. J.R. Mutton, S. M.Z. Di Mauro

Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have identified both the α and β subunits of this e.. Read More»

Genet. Mol. Res. 2(4):
Microbial Genetics   Research Article

Genetic polymorphism at spinocerebellar ataxia 1 and 2 loci in Brazil

Authors: Stenio F.P. Duarte, Raquel S. Gestinari, M�¡rio Campos-Jr, M�¡rcia M.G. Pimentel, Marcelo A. Costa Lima

Dynamic mutation involves the expansion of a tandem arrayed DNA sequence that is polymorphic in the population. This mechanism is associated with neurological/neuromuscular disorders and the pathology depends on the extension of the repeated tract, with a specific threshold for each disease. We made a PCR-based characterizatio.. Read More»

Genet. Mol. Res. 2(4):
Animal Genetics   Research Article

Gene flow between cassava, Manihot esculenta Crantz, and wild relatives

Authors: N.M.A. Nassar

Controlled and natural hybridization between cassava and wild relatives does occur. Barriers within the genus appear to be weak due to recent evolution of the group. All Manihot species examined cytogenetically have a chromosome number of 2n = 36. However, they behave meiotically as diploids. The weak interspecific barriers ha.. Read More»

Genet. Mol. Res. 2(4):
Microbial Genetics   Research Article

Molecular cytogenetic assignment of genes to bovine chromosome 5

Authors: Marcos De Donato, Dan S. Gallagher Jr., Cathy Lehn, Clare Gill, Jerry F. Taylor

Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignmen.. Read More»

Genet. Mol. Res. 2(3):
Animal Genetics   Research Article

Effects of crude extracts of Agaricus blazei on DNA damage and on rat liver carcinogenesis induced by diethylnitrosamine

Authors: Lu�­s Fernando Barbisan, Clarissa Scolastici, Maristela Miyamoto, Daisy Maria Favero Salvadori, L�ºcia Regina Ribeiro, Augusto Ferreira da Eira, Jo�£o Lauro Viana de Camargo

The effects of crude extracts of the mushroom Agaricus blazei Murrill (Agaricaceae) on both DNA damage and placental form glutathione S-transferase (GST-P)-positive liver foci induced by diethylnitrosamine (DEN) were investigated. Six groups of adult male Wistar rats were used. For two weeks, animals of groups 3 to 6 were trea.. Read More»

Genet. Mol. Res. 2(3):