Gene
Inheritance of resistance to Meloidogyne incognita race 2 in the hot pepper cultivar Carolina Cayenne(Capsicum annuum L.)
Authors: Fausto de Souza-Sobrinho, Wilson Roberto Maluf, Luiz A.A. Gomes and Vicente Paulo Campos
Root-knot nematodes of the genus Meloidogyne are important pathogens affecting vegetable crop production in Brazil and worldwide. The pepper species Capsicum annuum includes both hot and sweet peppers; very little emphasis has been placed on breeding sweet peppers for nematode resistance. We report on the inheritance of resist.. Read More»
- Genet. Mol. Res. 1(3):
Greater genetic variability in Argentine Creole than in Thoroughbred horses based on serum protein polymorphisms
Authors: S. D?az, F.N. Dulout and P. Peral-Garc?a
Genetic polymorphism was analyzed for five blood proteins: albumin - Al, esterase - Es, α1B-glycoprotein - Xk, transferrin - Tf and hemoglobin - Hb in 200 Thoroughbred (TB) and 124 Argentine Creole (AC) horses. Of the five systems examined, Tf and Hb were not in Hardy-Weinberg equilibrium in either breed and Es was not i.. Read More»
- Genet. Mol. Res. 1(3):
CENP-B is a conserved gene among vegetal
Authors: Olga Barbosa-Cisneros and Rafael Herrera-Esparza
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a .. Read More»
- Genet. Mol. Res. 1(3):
Genetic transformation of the lettuce cultivar Grand Rapids (Lactuca sativa L.) by Agrobacterium tumefaciens to improve osmotic stress tolerance (Transforma?§?£o gen?©tica de alface (Lactuca sativa L.), cultivar â??Grand Rapidsâ?, via Agrobacterium tumefaciens, para melhoramento de toler?¢ncia ao estresse h?drico)
Authors: Marcos Pileggi
-.. Read More»
- Genet. Mol. Res. 1(2):
A model system for testing gene vectors using murine tumor cells on the chorioallantoic membrane of the chick embryo
Authors: Sergio U. Dani and Rachel Espindola
We developed a model system for testing gene vectors, based on the growth of murine tumors on the chorioallantoic membrane (CAM) of embryonic chickens. The ability of selected murine cells to grow on the CAM was rated according to the following criteria: i) formation of tumor masses; ii) metastasis formation; iii) reproducibil.. Read More»
- Genet. Mol. Res. 1(2):
A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies
Authors: Ricardo Moratelli, Cl?¡udio de Moraes Andrade and Jorge Lu?s Azevedo de Armada
We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary .. Read More»
- Genet. Mol. Res. 1(2):
Molecular cytogenetics in metaphase and interphase cells for cancer and genetic research,diagnosis and prognosis. Application in tissue sections and cell suspensions
Authors: Maria M?¼hlmann
As the pioneer among molecular cytogenetics techniques, fluorescence in situ hybridization (FISH) allows identification of specific sequences in a structurally preserved cell, in metaphase or interphase. This technique, based on the complementary double-stranded nature of DNA, hybridizes labeled specific DNA (probe). The probe.. Read More»
- Genet. Mol. Res. 1(2):
Rapid identification of single nucleotide polymorphisms by fluorescence-based capillary electrophoresis
Authors: Montse Bernat, Esther Titos and Joan Cl? ria
We describe the application of two different fluorescence- based techniques (ddNTP primer extension and single-strand conformation polymorphism (SSCP)) to the detection of single nucleotide polymorphisms (SNPs) by capillary electrophoresis. The ddNTP primer extension technique is based on the extension, in the presence of fluo.. Read More»
- Genet. Mol. Res. 1(1):
Further phenotypic characterization of pso mutants of Saccharomyces cerevisiae with respect to DNA repair and response to oxidative stress
Authors: Cristina Pungartnik, Jaqueline Picada, Martin Brendel and Jo?£o A.P. Henriques
The sensitivity responses of seven pso mutants of Saccharomyces cerevisiae towards the mutagens N-nitrosodiethylamine (NDEA), 1,2:7,8-diepoxyoctane (DEO), and 8-hydroxyquinoline (8HQ) further substantiated their allocation into two distinct groups: genes PSO1 (allelic to REV3), PSO2 (SNM1), PSO4 (PRP19), and PSO5 (RAD16) const.. Read More»
- Genet. Mol. Res. 1(1):
Cystic fibrosis in Uruguay
Authors: Gerardo Luzardo, Isabel Aznarez, Beatriz Crispino, Adriana Mimbacas, Liria Mart?nez, Rossana Poggio, Julian Zielenski, Lap-Chee Tsui and Horacio Cardoso
We conducted clinical and genetic analyses of 52 cystic fibrosis (CF) patients in Uruguay, which is about half of the known affected individuals in the country. A relatively high proportion had a mild presentation, characterized by pancreatic sufficiency (28%), a strong pulmonary component (97%), and borderline sweat electroly.. Read More»
- Genet. Mol. Res. 1(1):