Genetics
Evaluation of hot saline solution and restriction endonuclease techniques in cytogenetic studies of Cycloneda sanguinea L. (Coccinellidae)
Authors: Maffei EMD and Pompolo SG
The goal of the present study was to determine if simple methods, especially hot saline solution (HSS) and MspI and HaeIII restriction endonucleases, which do not require special equipments, may be helpful in studies of genetic variability in the lady beetle, Cycloneda sanguinea. The HSS method extracted the heterochromatin re.. Read More»
- Genet. Mol. Res. 6(1):
Accurate monitoring of promoter gene methylation with high-resolution melting polymerase chain reaction using the ABCB1 gene as a model
Authors: A.L. Mencalha, E.F. Rodrigues, E. Abdelhay and T.S. Fernandez
Multidrug resistance is the major cause of cancer chemotherapy failure. This phenotype is mainly due to the overexpression of the human ABCB1 gene. Several studies have shown that the transcriptional regulation of this gene is complex. Yet, the impact of this transcriptional regulation has not been well studied in a clinical setting. The acquired expressi.. Read More»
- Genet. Mol. Res. 12(1):
- 2013
- DOI:
- 10.4238/2013
Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae)
Authors: Y. Wang, H. Lu, J. Zheng, K. Long, Y.H. Shi, Z.F. Gu and A.M. Wang
The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.21.5
- DOI:
- 10.4238/2011.December.21.5
Molecular differentiation in natural populations of Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Brazilian Amazon, using sequences of the COI gene from mitochondrial DNA
Authors: V.M. Scarpassa and J.E. Conn
Anopheles (Nyssorhynchus) oswaldoi (Peryassú, 1922) s. l., which has been incriminated as a potential human malaria vector in Western Brazilian Amazon, may constitute a cryptic species complex. However, the most recent study with isozymes indicated high similarity among samples from the States of Acre, Amazonas and Rond.. Read More»
- Genet. Mol. Res. 5(3):
Manipulation of primer affinity improves high-resolution melting accuracy for imprinted genes
Authors: F.V.M. Rubatino, N.V. Carobin, M.L. Freitas, V.T. de Oliveira, R.X. Pietra, P.P.R. Oliveira, A.A. Bosco and F.S. Jehee
High-resolution melting (HRM) is considered an inexpensive, rapid, and attractive methodology for methylation analysis. In the application of the polymerase chain reaction (PCR) to methylation analysis, amplification efficiencies are biased towards unmethylated, rather than methylated, templates: a phenomenon known as PCR bias. To overcome PCR bias, prime.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.14.12
- DOI:
- 10.4238/2015.July.14.12
Occurrence of natural triploidy in Rhamdia quelen (Siluriformes, Heptapteridae)
Authors: J.R. Tsuda, V.P.O. de Moraes, L. Giuliano-Caetano and A.L. Dias
Five specimens of Rhamdia quelen collected from the Lindóia Stream, PR, Brazil, were cytogenetically analyzed. The diploid chromosome number found was 58, including 30 metacentric, 16 submetacentric, 10 subtelocentric, and 2 acrocentric chromosomes. Supernumerary or B chromosomes, frequently observed in this fish group,.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr949
- DOI:
- 10.4238/vol9-3gmr949
Genetic diversity and relatedness between Canind�© and British Alpine goat breeds in Northeastern Brazil accessed by microsatellite markers
Authors: T.S. C�¢mara, J.F. Nunes, F.M. Diniz, G.R. Silva, A.M. Ara�ºjo
The aim of this study was to access the genetic diversity and relatedness between CanindÃ?© and British Alpine goat breeds in the States of PiauÃ? and CearÃ?¡ using microsatellite markers. Genomic DNA was isolated from hair samples of 99 goats belonging to six different flocks. A panel of polymorphic heterologous microsatellite loci was used to genotype .. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019569
- DOI:
- 10.4238/gmr16019569
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
Authors: C.A.F. Melo, G.S. Silva and M.M. Souza
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prep.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.4
- DOI:
- 10.4238/2015.March.27.4
A novel polymorphic Alu insertion embedded in a LINE 1 retrotransposon in the human X chromosome (DXS225): identification and worldwide population study
Authors: Rinaldo Wellerson Pereira, Simone Silva dos Santos and S�©rgio Danilo Junho Pena
We describe a novel polymorphic Alu insertion (DXS225) on the human X chromosome (Xq21.3) embedded into an L1 retrotransposon. The DXS225 polymorphism was genotyped in 684 males from the CEPH Human Genome Diversity Panel. This insertion was found in all regions of the globe, suggesting that it took place before modern humans s.. Read More»
- Genet. Mol. Res. 5(1):
A comparative analysis of highly conserved sex-determining genes between Apis mellifera and Drosophila melanogaster
Authors: Alexandre S. Cristino, Adriana Mendes do Nascimento, Luciano da Fontoura Costa and Zil�¡ Luz Paulino Sim�µes
A comparison of the most conserved sex-determining genes between the fruit fly, Drosophila melanogaster, and the honey bee, Apis mellifera, was performed with bioinformatics tools developed for computational molecular biology. An initial set of protein sequences already described in the fruit fly as participants of the sex-det.. Read More»
- Genet. Mol. Res. 5(1):