Authors: V.B. Bardella, M.T.V. Azeredo-Oliveira and E. Tartarotti
Triatomines are of great concern in public health because they are vectors of Chagas’ disease. This study presents an analysis of the species Triatoma melanosoma. The cytogenetic characteristics of triatomines include holocentric chromosomes, post-reductional meiosis in the sex chromosomes and nucleolar fragmentation in .. Read More»
Authors: D.C.S. Sena and W.F. Molina
The parrotfishes (family Scaridae) are comprised of the subfamilies Sparisomatinae and Scarinae. They are important agents of marine bioerosion, which rework the substrate with their beaklike jaws. Despite their importance, there are no published cytogenetic data on this group. We made cytogenetic analyses of Sparisoma axillar.. Read More»
Authors: I.G. Hamoy, E.J.M. Santos and S.E.B. Santos
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtain.. Read More»
Authors: H. Wang, X. Luo, W.B. Shi and B.W. Zhang
Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information c.. Read More»
Authors: T.C. Oliveira1,3, A.B.R. Santos2, K.C.N. Rabelo1,3, C.A. Souza3,S.M. Santos3 and S. Crovella1
The use of insects to answer questions in criminal investigations, as well as a combination of forensic genetic techniques to obtain human DNA from the organisms, especially necrophagous dipterians, have gained ground in recent decades among researchers and professionals in this area. The objective of our study was to evaluate.. Read More»
Authors: L. Cifuentes, H. Jorquera, M. Acu�±a, J. Ord�³�±ez and A.L. Sierra
Two hundred and sixty unrelated subjects who asked for paternity testing at two Bolivian Laboratories in La Paz and Santa Cruz were studied. The loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, and CSF1PO were typed from blood samples, amplifying DNA by polymerase chain reactions and electr.. Read More»
Authors: J.R. Aarestrup, D. Karam and G.W. Fernandes
Euphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome number, morphology, and behavior of mitotic chromosomes. Seeds were germinated and subm.. Read More»
Authors: L.A. Peixoto, L.L. Bhering and C.D. Cruz
Genomic selection is a useful technique to assist breeders in selecting the best genotypes accurately. Phenotypic selection in the F2 generation presents with low accuracy as each genotype is represented by one individual; thus, genomic selection can increase selection accuracy at this stage of the breeding program. This study.. Read More»
Authors: R.F. Sun, Y.S. Zhu, J.L. Feng, Z. Tian, W.J. Kuang, Y. Liu, H.B. Zhang and S.B. Li
To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and.. Read More»
Authors: L.O.S. Anjos, R.F. Peixoto Jr, D.M.S. Chanquinie, L.R. Pinto,S.A. Creste, L.L. Dinardo-Miranda, M.G.A. Landell, E.V. Tambarussi and R. Vencovsky
Cotesia flavipes (Cameron) is a parasitoid wasp used in the biological control of the sugarcane borer (Diatraea saccharalis) (Fabr., 1794). Studies on the genetic diversity of C. flavipes are hampered by the lack of highly polymorphic molecular markers. In this report, a set of 11 microsatellite loci were developed from an enr.. Read More»