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Genetics

Animal Genetics   Research Article

Cytogenetic analysis in the spermatogenesis of Triatoma melanosoma (Reduviidae; Heteroptera)

Authors: V.B. Bardella, M.T.V. Azeredo-Oliveira and E. Tartarotti

Triatomines are of great concern in public health because they are vectors of Chagas’ disease. This study presents an analysis of the species Triatoma melanosoma. The cytogenetic characteristics of triatomines include holocentric chromosomes, post-reductional meiosis in the sex chromosomes and nucleolar fragmentation in .. Read More»

Genet. Mol. Res. 7(2):
vol7-2gmr411
DOI:
10.4238/vol7-2gmr411
Animal Genetics   Research Article

Robertsonian rearrangements and pericentric inversions in Scaridae fish (Perciformes)

Authors: D.C.S. Sena and W.F. Molina

The parrotfishes (family Scaridae) are comprised of the subfamilies Sparisomatinae and Scarinae. They are important agents of marine bioerosion, which rework the substrate with their beaklike jaws. Despite their importance, there are no published cytogenetic data on this group. We made cytogenetic analyses of Sparisoma axillar.. Read More»

Genet. Mol. Res. 6(3):
vol7-2erratagmr32
DOI:
10.4238/vol7-2erratagmr32
Cytogenomics   Research Article

Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites

Authors: I.G. Hamoy, E.J.M. Santos and S.E.B. Santos

The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtain.. Read More»

Genet. Mol. Res. 7(1):
vol7-1gmr394
DOI:
10.4238/vol7-1gmr394
Human Genetics   Research Article

Isolation and characterization of polymorphic microsatellite loci of the Chinese muntjac (Muntiacus reevesi)

Authors: H. Wang, X. Luo, W.B. Shi and B.W. Zhang

Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information c.. Read More»

Genet. Mol. Res. 13(1):
http://dx.doi.org/2014.March.17.18
DOI:
http://dx.doi.org/10.4238/2014.March.17.18
Human Genetics   Research Article

Human autosomal DNA and X chromosome STR profiles obtained from Chrysomya albiceps (Diptera: Calliphoridae) larvae used as a biological trace

Authors: T.C. Oliveira1,3, A.B.R. Santos2, K.C.N. Rabelo1,3, C.A. Souza3,S.M. Santos3 and S. Crovella1

The use of insects to answer questions in criminal investigations, as well as a combination of forensic genetic techniques to obtain human DNA from the organisms, especially necrophagous dipterians, have gained ground in recent decades among researchers and professionals in this area. The objective of our study was to evaluate.. Read More»

Genet. Mol. Res. 15(4):
gmr15047622
DOI:
10.4238/gmr15047622
Plant Genetics   Research Article

Allele frequencies for 12 autosomal short tandem repeat loci in two bolivian populations

Authors: L. Cifuentes, H. Jorquera, M. Acu�±a, J. Ord�³�±ez and A.L. Sierra

Two hundred and sixty unrelated subjects who asked for paternity testing at two Bolivian Laboratories in La Paz and Santa Cruz were studied. The loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, and CSF1PO were typed from blood samples, amplifying DNA by polymerase chain reactions and electr.. Read More»

Genet. Mol. Res. 7(1):
vol7-1gmr368
DOI:
10.4238/vol7-1gmr368
Animal Genetics   Research Article

Chromosome number and cytogenetics of Euphorbia heterophylla L.

Authors: J.R. Aarestrup, D. Karam and G.W. Fernandes

Euphorbia heterophylla L. (Euphorbiaceae) is a herbaceous species of great economic importance due to its invasive potential and consequent damage to agriculture and pasture land. For the first time, we provide information on its chromosome number, morphology, and behavior of mitotic chromosomes. Seeds were germinated and subm.. Read More»

Genet. Mol. Res. 7(1):
vol7-1gmr421
DOI:
10.4238/vol7-1gmr421
Human Genetics   Research Article

Determination of the optimal number of markers and individuals in a training population necessary for maximum prediction accuracy in F2 populations by using genomic selection models

Authors: L.A. Peixoto, L.L. Bhering and C.D. Cruz

Genomic selection is a useful technique to assist breeders in selecting the best genotypes accurately. Phenotypic selection in the F2 generation presents with low accuracy as each genotype is represented by one individual; thus, genomic selection can increase selection accuracy at this stage of the breeding program. This study.. Read More»

Genet. Mol. Res. 15(4):
gmr15048874
DOI:
10.4238/gmr15048874
Medical Genetics   Research Article

Polymorphisms of three new microsatellite sites of the dystrophin gene

Authors: R.F. Sun, Y.S. Zhu, J.L. Feng, Z. Tian, W.J. Kuang, Y. Liu, H.B. Zhang and S.B. Li

To look for novel microsatellites in the dystrophin gene for the diagnosis of Duchenne muscular dystrophy, candidate microsatellite sites in the dystrophin gene were analyzed with the SSRHunter software and were also genotyped. Among the 15 candidate microsatellite sites, three novel microsatellite sites in the 60th, 30th, and.. Read More»

Genet. Mol. Res. 10(2):
vol10-2gmr962
DOI:
10.4238/vol10-2gmr962
Plant Genetics   Research Article

Microsatellite loci and genetic structure of artificial populations of Cotesia flavipes (Hymenoptera, Braconidae)

Authors: L.O.S. Anjos, R.F. Peixoto Jr, D.M.S. Chanquinie, L.R. Pinto,S.A. Creste, L.L. Dinardo-Miranda, M.G.A. Landell, E.V. Tambarussi and R. Vencovsky

Cotesia flavipes (Cameron) is a parasitoid wasp used in the biological control of the sugarcane borer (Diatraea saccharalis) (Fabr., 1794). Studies on the genetic diversity of C. flavipes are hampered by the lack of highly polymorphic molecular markers. In this report, a set of 11 microsatellite loci were developed from an enr.. Read More»

Genet. Mol. Res. 15(4):
gmr.15048851
DOI:
10.4238/gmr.15048851