Genomics
Pharmacogenomics: accessing important alleles by imputation from commercial genome-wide SNP arrays
Authors: R. Liboredo and S.D.J. Pena
Personalized medicine is becoming a medical reality, as important genotype-phenotype relationships are being unraveled. The availability of pharmacogenomic data is a key element of individualized care. In this study, we explored genotype imputation as a means to infer important pharmacogenomic alleles from a regular commercial.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.27
- DOI:
- 10.4238/2014.July.25.27
Use of PCR-DHPLC with fluorescence detection for the characterization of the bacterial diversity during cassava (Manihot esculenta crantz) fermentation
Authors: C.S. Kodama, S. Cuadros-Orellana, C.H.M.M. Bandeira, D.A. Gra�§as, A.S. Santos and A. Silva
Denaturing high-performance liquid chromatography (DHPLC) has been described as a suitable method to study DNA polymorphisms. Here, cassava (Manihot esculenta Crantz) fermentation liquor was examined using DHPLC analysis to characterize the bacterial diversity during the fermentation process. GC-clamped amplicons corresponding to a variable region of the .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.February.28.2
- DOI:
- http://dx.doi.org/10.4238/2014.February.28.2
Fluorescence Image Analyzer - FLIMA: software for quantitative analysis of fluorescence in situ hybridization
Authors: H.C.M. Silva, M.M.C. Martins-J�ºnior, L.B. Ribeiro, D.A. Matoso
The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the a.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019658
- DOI:
- 10.4238/gmr16019658
Prevalence of CYP2C9 and VKORC1 alleles in the Argentine population and implications for prescribing dosages of anticoagulants
Authors: P. Scibona, M.A. Redal, L.G. Garfi, J. Arbelbide, P.F. Argibay and W.H. Belloso
Dicumarinic oral anticoagulants have a narrow therapeutic range and a great individual variability in response, which makes calculation of the correct dose difficult and critical. Genetic factors involved in this variability include polymorphisms of genes that encode the metabolic enzyme CYP2C9 and the target enzyme vitamin K .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.9.8
- DOI:
- http://dx.doi.org/10.4238/2012.January.9.8
Parameters of a dynamic mechanistic model of cattle growth retain enough biological interpretation for genotype-to-phenotype mapping
Authors: M.C. Freua, M.H.A. Santana R.V. Ventura and J.B.S. Ferraz
This study aimed to investigate the predictability of a phenotype when using a dynamic model of cattle growth. Genotypic and phenotypic information on Nellore (Bos indicus) cattle were used in a genome-wide association analysis designed to contrast the biological interpretation of core parameters [conversion efficiency of meta.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048931
- DOI:
- 10.4238/gmr15048931
In silico prediction of conserved vaccine targets in Streptococcus agalactiae strains isolated from fish, cattle, and human samples
Authors: U.P. Pereira, S.C. Soares, J. Blom, C.A.G. Leal, R.T.J. Ramos, L.C. Guimar�£es, L.C. Oliveira, S.S. Almeida, S.S. Hassan, A.R. Santos, A. Miyoshi, A. Silva, A. Tauch, D. Barh, V. Azevedo and H.C.P. Figueiredo
Streptococcus agalactiae (Lancefield group B; group B streptococci) is a major pathogen that causes meningoencephalitis in fish, mastitis in cows, and neonatal sepsis and meningitis in humans. The available prophylactic measures for conserving human and animal health are not totally effective and have limitations. Effective vaccines against the different .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.6
- DOI:
- 10.4238/2013.August.12.6
Composition and structure of microbial communities associated with different domestic sewage outfalls
Authors: Z.H. Wang, J.Q. Yang, D.J. Zhang, J. Zhou, C.D. Zhang, X.R. Su and T.W. Li
The diversity of microbiota in waste waters has not been thoroughly examined, despite the potential impact of microbes on effluent quality. Wastewater microbial communities harbor pathogenic bacteria, viruses, and parasites. To study microbial communities in domestic sewage outfalls, 454 pyrosequencing technology was used to i.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.21
- DOI:
- 10.4238/2014.September.12.21
Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease
Authors: N.F. Rodrigues, J. K�¤stle, T.J.D. Coutinho, A.T. Amorim, G.B. Campos, V.M. Santos, L.M. Marques, J. Timenetsky and S.T. de Farias1
The microbial community of the reproductive apparatus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic information about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.12.4
- DOI:
- 10.4238/2015.June.12.4
Effect of glycerol on GHR and IGF-1 gene expression in breast muscle and on growth of Japanese meat quails
Authors: S.C.C. Silva, E. Gasparino, D.M. Voltolini, A.V. Pires, F. Tanamati, A.L.S. Garcia and D.P. de Oliveira
We evaluated messenger RNA (mRNA) expression of the growth-hormone (GHR) and insulin-like growth factor (IGF-1) genes in 28-day-old Japanese meat quails fed diets containing 0, 8, or 12% dietary glycerol in substitution of corn. Total RNA was extracted from the breast muscle and the DNA was amplified with specific primers using real-time PCR. Feed convers.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.3
- DOI:
- 10.4238/2013.September.23.3
Multiple-trait genomic evaluation for milk yield and milk quality traits using genomic and phenotypic data in buffalo in Brazil
Authors: R.R. Aspilcueta-Borquis, F.R. Araujo Neto, D.J.A. Santos, N.A. Hurtado-Lugo, J.A.V. Silva and H. Tonhati
The objective of this study was to compare the multi-trait model using pedigree information and a model using genomic information in addition to pedigree information. We used data from 5896 lactations of 2021 buffalo cows, of which 384 were genotyped using the Illumina Infinium® bovine HD BeadChip, considering seven traits related to milk yield (MY305.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.27
- DOI:
- 10.4238/2015.December.22.27