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Germplasm

Plant Genetics   Research Article

Comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of Vitis vinifera

Authors: G.C. Santâ??Ana, J.L. Ferreira, H.S. Rocha, A. Bor?©m, M. Pasqual and G.M.A. Can?§ado

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiat.. Read More»

Genet. Mol. Res. 11(2):
2012.May.21.8
DOI:
10.4238/2012.May.21.8
Microbial Genetics   Research Article

Analysis of genetic diversity of salt-tolerant alfalfa germplasms

Authors: J. Jiang, B.L. Yang, T. Xia, S.M. Yu, Y.N. Wu, H. Jin and J.R. Li

Random amplified polymorphic DNA technology was used to analyze the genetic diversity of 25 salt-tolerant alfalfa varieties using 30 different primers. Results showed that the percentage of polymorphic loci between single-plant DNA was 81.52%, and that between mixed DNA of various varieties was 61.65%. Compared to the mixed DNA samples, single-plant DNA s.. Read More»

Genet. Mol. Res. 14(2):
2015.May.4.1
DOI:
10.4238/2015.May.4.1
Plant Genetics   Research Article

Development of a novel set of microsatellite markers for Lippia alba (Verbenaceae)

Authors: D.S. Rocha, C.P. Santos, M.M. Bajay, J.B. Campos, A.F. Blank,J.B. Pinheiro and M.I. Zucchi

Microsatellite primers were developed and optimized for Lippia alba to characterize the L. alba germplasm bank of Universidade de S?£o Paulo. A genomic library enabled the design of 9 microsatellite primers. Six of the 9 primers yielded polymorphic products, which defined 2 groups in the bank. The data provide support to characterize germplasm banks, genet.. Read More»

Genet. Mol. Res. 14(1):
2015.February.3.4
DOI:
10.4238/2015.February.3.4
Plant Genetics   Research Article

Variability and inbreeding in semiexotic maize populations

Authors: A.S. Oliveira, J.B. Miranda Filho and E.F. Reis

Three semiexotic populations (CRE-01, CRE-02, CRE-03) obtained by incorporation of exotic germplasm (lines from CIMMYT, Colombia; selected for resistance to corn stunt complex) were evaluated in two cycles of recurrent selection with half-sib families. In cycle-I, samples comprising 50, 70, and 50 half-sib families were evaluated at Jataí (GO) and .. Read More»

Genet. Mol. Res. 14(1):
2015.February.6.21
DOI:
10.4238/2015.February.6.21
Plant Genetics   Research Article

Genetic diversity in tetraploid switchgrass revealed by AFLP marker polymorphisms

Authors: J. Todd, Y.Q. Wu, Z. Wang and T. Samuels

Switchgrass (Panicum virgatum) is a perennial warm-season grass native to North America that has been identified as a dedicated cellulosic biofuel crop. We quantified genetic diversity in tetraploid switchgrass germplasm collected at Oklahoma State University and characterized genetic relatedness among the collections from dis.. Read More»

Genet. Mol. Res. 10(4):
2011.November.29.8
DOI:
10.4238/2011.November.29.8
Plant Genetics   Research Article

Genetic diversity and phylogenetic relationship among Tunisian cactus species (Opuntia) as revealed by random amplified microsatellite polymorphism markers

Authors: M. Bendhifi Zarroug, G. Baraket, L. Zourgui, S. Souid and A. Salhi Hannachi

Opuntia ficus indica is one of the most economically important species in the Cactaceae family. Increased interest in this crop stems from its potential contribution to agricultural diversification, application in the exploitation of marginal lands, and utility as additional income sources for farmers. In Tunisia, O. ficus indica has been affected by dras.. Read More»

Genet. Mol. Res. 14(1):
2015.February.13.21
DOI:
10.4238/2015.February.13.21
Plant Genetics   Research Article

Genetic diversity of worldwide Jerusalem artichoke (Helianthus tuberosus) germplasm as revealed by RAPD markers

Authors: P.P. Wangsomnuk, S. Khampa, P. Wangsomnuk, S. Jogloy, T. Mornkham, B. Ruttawat, A. Patanothai and Y.B. Fu

Jerusalem artichoke (Helianthus tuberosus) is a wild relative of the cultivated sunflower (H. annuus); it is an old tuber crop that has recently received renewed interest. We used RAPD markers to characterize 147 Jerusalem artichoke accessions from nine countries. Thirty RAPD primers were screened; 13 of them detected 357 repr.. Read More»

Genet. Mol. Res. 10(4):
2011.December.12.4
DOI:
10.4238/2011.December.12.4
Microbial Genetics   Research Article

Genetic diversity analysis of tree peony germplasm using iPBS markers

Authors: Y.B. Duan, D.L. Guo, L.L. Guo, D.F. Wei and X.G. Hou

We examined the genetic diversity of 10 wild species (populations) and 55 varieties of tree peony using inter-primer binding site (iPBS) markers. From a total of 36 iPBS primers, 16 were selected based on polymorphic amplification. The number of bands amplified by each primer ranged from 9 to 19, with an average of 12.88 bands per primer. The length of ba.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.31
DOI:
10.4238/2015.July.3.31
Plant Genetics   Research Article

Collinearity analysis of allotetraploid Gossypium tomentosum and Gossypium darwinii

Authors: F. Liu, Z.L. Zhou, C.Y. Wang, Y.H. Wang, X.Y. Cai, X.X. Wang, K.B. Wang and Z.S. Zhang

Gossypium tomentosum and G. darwinii are wild allotetraploid cotton species, characterized by many excellent traits, including fiber fineness, drought tolerance, and Fusarium and Verticillium wilt resistance. Based on the construction of F2 linkage groups of G. hirsutum x G. tomentosum and G. hirsutum x G. darwinii, two genetic linkage maps were compared... Read More»

Genet. Mol. Res. 15(3):
gmr.15038391
DOI:
10.4238/gmr.15038391
Microbial Genetics   Research Article

Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers

Authors: ?.M. Vidal L.J. Vieira, C.F. Ferreira, F.V.D. Souza, A.S. Souza and C.A.S. Ledo

Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits.. Read More»

Genet. Mol. Res. 14(3):
2015.July.14.2
DOI:
10.4238/2015.July.14.2