Goat
Analysis of imprinted messenger RNA expression in deceased transgenic cloned goats
Authors: R.X. Jia, Z.R. Zhou, G.M. Zhang, L.Z. Wang, Y.X. Fan, Y.J. Wan, Y.L. Zhang,Z.Y. Wang and F. Wang
Genomic imprinting is an important epigenetic mechanism that has vital effects on fetal growth and development. We observed the differences in four tissues (heart, spleen, liver, and kidney) from dead transgenic cloned goats using hematoxylin and eosin (H&E) staining. Eight imprinted genes in the tissues of dead transgenic cloned and normal goats were.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017455
- DOI:
- 10.4238/gmr.15017455
Analysis of POU1F1 gene DdeI polymorphism in Chinese goats
Authors: M.J. Li1,2, C.M. Zhang2, X.Y. Lan1, X.T. Fang3, C.Z. Lei1 and H. Chen1
As a member of the POU-domain family, the POU1F1 is a positive regulator for growth hormone, prolactin and thyroid-stimulating hormone b, by binding to target DNA promoters as a dimer in mammals. This study described the polymorphisms at the goat POU1F1-DdeI locus and analyzed the distribution of alleles in 15 indigenous Chinese goat breeds. The PCR-RFLP .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017747
- DOI:
- 10.4238/gmr.15017747
AGPAT6 polymorphism and its association with milk traits of dairy goats
Authors: C. He, C. Wang, Z.H. Chang, B.L. Guo, R. Li, X.P. Yue, X.Y. Lan, H. Chen and C.Z. Lei
As one of the eight members in the 1-acylglycerol-3-phosphate-O-acyltransferase (AGPATs) family, AGPAT6 is a crucial enzyme for the biosynthesis of glycerolipids and triacylglycerol in eukaryotes, as well as catalyzing the conversion from lysophosphatidic acid to phosphatidic acid. AGPAT6 can be considered as a candidate gene .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.4.8
- DOI:
- 10.4238/2011.November.4.8
Identification of transgenic cloned dairy goats harboring human lactoferrin and methylation status of the imprinted gene IGF2R in their lungs
Authors: Y.L. Zhang, G.M. Zhang, Y.J. Wan, R.X. Jia, P.Z. Li, L. Han, F. Wang and M.R. Huang
Dairy goat is a good model for production of transgenic proteins in milk using somatic cell nuclear transfer (SCNT). However, animals produced from SCNT are often associated with lung deficiencies. We recently produced six transgenic cloned dairy goats harboring the human lactoferrin gene, including three live transgenic clones and three deceased transgen.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.3
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.3
Growth and viability of Liaoning Cashmere goat hair follicles during the annual hair follicle cycle
Authors: Q.L. Zhang, J.P. Li, Y. Chen, Q. Chang, Y.M. Li, J.Y. Yao, H.Z. Jiang, Z.H. Zhao and D. Guo
Here, we studied hair follicle development of Liaoning Cashmere goats. Every month for 1 year, skin samples were collected from five 1.5-year-old female goats, and made into paraffin sections. A number of parameters were measured of primary and secondary hair follicles via microscopic observation including follicle depth, hair.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.16.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.16.2
Cloning, molecular characterization, and expression pattern of FGF5 in Cashmere goat (Capra hircus)
Authors: W.L. Bao, R.Y. Yao, Q. He, Z.X. Guo, C. Bao, Y.F. Wang and Z.G. Wang
Fibroblast growth factor 5 (FGF5) is a secreted signaling protein that belongs to the FGF family, and was found to be associated with hair growth in humans and other animals. The Inner Mongolia Cashmere goat (Capra hircus) is a goat breed that provides superior cashmere; this breed was formed by spontaneous mutation in China. Here, we report the cloning, .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.9
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.9
Associations between polymorphisms of the GFI1B gene and growth traits of indigenous Chinese goats.
Authors: H.F. Cai, Z. Chen and W.X. Luo
This study aimed to investigate polymorphisms of the eighth exon in the GFI1B gene among three indigenous Chinese goat breeds (QianBei Ma goats, GuiZhou white goats, and GuiZhou black goats). Furthermore, association analysis was conducted between these polymorphisms and growth traits. Polymerase chain reaction-single strand conformation polymorphism (PCR.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.13.5
- DOI:
- 10.4238/2014.February.13.5
A novel genetic variant of the goat Six6 gene and its association with production traits in Chinese goat breeds
Authors: C.Y. Pan, X.Y. Lan, H.Y. Zhao, S.R. Hu, Y.T. Huai, C.Z. Lei and H. Chen
We looked for novel genetic variations within the Six6 gene by PCR-SSCP, DNA sequencing and forced RFLP-PCR and estimated their associations with production traits in 2132 goats of eight indigenous Chinese breeds. A novel single nucleotide polymorphism (NM_001104993.1: g.232T>C) within the goat Six6 gene was identified. The.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.22.9
- DOI:
- 10.4238/2011.November.22.9
A novel polymorphism of the lactoferrin gene and its association with milk composition and body traits in dairy goats
Authors: B.L. Guo, Y. Jiao, C. He, L.X. Wei, Z.H. Chang, X.P. Yue, X.Y. Lan, H. Chen and C.Z. Lei
Milk composition and body measurement traits, influenced by genes and environmental factors, play important roles in value assessments of efficiency and productivity in dairy goats. Lactoferrin (LF), involved in the efficient expression of protein in milk, is also an anabolic factor in skeletal tissue and a potent osteoblast s.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr928
- DOI:
- 10.4238/vol9-4gmr928
Application of the Sleeping Beauty system in Saanen goat fibroblast cells for establishing persistent transgene expression
Authors: B.C. Jiang, H.A. Kaleri, H.X. Zhang, J. Chen and H.L. Liu
The Sleeping Beauty (SB) transposon system is a promising new method for establishing persistent transgene expression in vivo. We applied the SB system for enhancing transgenesis in Saanen dairy goat fibroblast cells. We constructed a pKT2/CMV-EGFP-IRES-PURO vector and investigated the influence of transposon and transposase v.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.25.4
- DOI:
- 10.4238/2011.October.25.4